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Literature summary extracted from

  • Junca, H.; Plumeier, I.; Hecht, H.J.; Pieper, D.H.
    Difference in kinetic behaviour of catechol 2,3-dioxygenase variants from a polluted environment (2004), Microbiology, 150, 4181-4187.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.2 expressed in Escherichia coli JM109 Pseudomonas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.2 0.0009
-
4-Chlorocatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 0.0011
-
catechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 0.0015
-
3-methylcatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 0.0016
-
4-Chlorocatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 0.0019
-
4-methylcatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 0.0025
-
catechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 0.0032
-
3-methylcatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 0.0052
-
4-methylcatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.2 35000
-
SDS-PAGE Pseudomonas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.2 alkylcatechol + O2 Pseudomonas sp. degradation of aromatic compounds ?
-
?
1.13.11.2 catechol + O2 Pseudomonas sp. degradation of aromatic compounds 2-hydroxymuconate semialdehyde
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.2 Pseudomonas sp. Q83U22 strains 1YB2 and 1YdBTEX2
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.2 3-methylcatechol + O2
-
Pseudomonas sp. 2-hydroxy-3-methylmuconate semialdehyde
-
?
1.13.11.2 4-chlorocatechol + O2
-
Pseudomonas sp. 4-chloro-2-hydroxymuconate semialdehyde
-
?
1.13.11.2 4-methylcatechol + O2
-
Pseudomonas sp. 2-hydroxy-4-methylmuconate semialdehyde
-
?
1.13.11.2 alkylcatechol + O2 degradation of aromatic compounds Pseudomonas sp. ?
-
?
1.13.11.2 catechol + O2
-
Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?
1.13.11.2 catechol + O2 degradation of aromatic compounds Pseudomonas sp. 2-hydroxymuconate semialdehyde
-
?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.13.11.2 17
-
3-methylcatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 17
-
4-Chlorocatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 25
-
3-methylcatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 38
-
catechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 45
-
4-Chlorocatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 53
-
4-methylcatechol strain 1YB2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 64
-
4-methylcatechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.
1.13.11.2 128
-
catechol strain 1YdBTEX2, pH 7.5, 25°C Pseudomonas sp.