EC Number | Cloned (Comment) | Organism |
---|---|---|
1.12.98.4 | the SR gene cluster encompasses five ORFs with a total length of 7389 bp. The sreA gene encodes the 110000 Da subunit of SR. sreB encodes an FeS protein, sreC encodes a hydrophobic protein with 10 predicted transmembrane helices. The sreD gene encodes a putative hydrophilic FeS protein with 26 cysteine residues and of unknown cluster composition. The sreE gene encodes a protein with similarity to reductase assembly proteins | Acidianus ambivalens |
1.12.99.6 | - |
Acidianus ambivalens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.12.98.4 | membrane | bound to | Acidianus ambivalens | 16020 | - |
1.12.99.6 | membrane | membrane bound | Acidianus ambivalens | 16020 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.12.99.6 | Fe | 0.134 mmol Fe per g protein | Acidianus ambivalens | |
1.12.99.6 | Mo | 0.0025 mmol Mo per g protein | Acidianus ambivalens | |
1.12.99.6 | Ni | 0.0025 mmol Ni per g protein | Acidianus ambivalens |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 41000 | - |
x * 41000 + x * 66000, SDS-PAGE | Acidianus ambivalens |
1.12.99.6 | 66000 | - |
x * 41000 + x * 66000, SDS-PAGE | Acidianus ambivalens |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.12.98.4 | Acidianus ambivalens | - |
- |
- |
1.12.99.6 | Acidianus ambivalens | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.12.98.4 | - |
Acidianus ambivalens |
1.12.99.6 | density-gradient centrifugation, column chromatography | Acidianus ambivalens |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.12.99.6 | 114 | - |
acceptor methyl viologen | Acidianus ambivalens |
1.12.99.6 | 624 | - |
acceptor 2,3-dimethylnaphthoquinol | Acidianus ambivalens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.12.98.4 | sulfur + H2 | in solubilized membrane fraction no activity is detected without the addition of electron carriers: 2,3-dimethylnaphthoquinone, plastoquinone or horse heart cytochrome c | Acidianus ambivalens | H2S | - |
? | |
1.12.99.6 | H2 + oxidized 2,3-dimethylnaphthoquinone | - |
Acidianus ambivalens | 2,3-dimethylnaphthoquinol | - |
? | |
1.12.99.6 | H2 + oxidized benzyl viologen | - |
Acidianus ambivalens | reduced benzyl viologen + H+ | - |
? | |
1.12.99.6 | H2 + oxidized methyl viologen | - |
Acidianus ambivalens | reduced methyl viologen + H+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.12.99.6 | ? | x * 41000 + x * 66000, SDS-PAGE | Acidianus ambivalens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.12.98.4 | 8 | - |
no activity is observed below pH 6.0, the activity gradually increases up to pH 8.0, above pH 8.0 the rate of non-enzymic sulfur disproportionation reaction is higher than the enzymic rate | Acidianus ambivalens |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.12.98.4 | 6 | 8 | no activity is observed below pH 6.0, the activity gradually increases up to pH 8.0, above pH 8.0 the rate of non-enzymic sulfur disproportionation reaction is higher than the enzymic rate | Acidianus ambivalens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.12.98.4 | NADPH | addition increases activity | Acidianus ambivalens |