EC Number | Cloned (Comment) | Organism |
---|---|---|
2.1.1.130 | gene cbiL, DNA and amino acid sequence determination and analysis, genetic organization, co-overexpression of enzymes and reconstruction of the anaerobic cobalamin biosynthetic pathway in Escherichia coli in a construct comprising several genes, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
2.1.1.130 | gene cbiL, DNA and amino acid sequence determination and analysis, genetic organization, co-overexpression of enzymes and reconstruction of the anaerobic cobalamin biosynthetic pathway in Escherichia coli in a construct comprising several genes, overview | Propionibacterium freudenreichii |
2.1.1.131 | gene cbiEGH, the gene cbiH of Propionibacterium freudenreichii is organized in a single ORF with genes cbiE and cbiG, DNA and amino acid sequence determination and analysis, genetic organization, co-overexpression of enzymes and reconstruction of the anaerobic cobalamin biosynthetic pathway in Escherichia coli in a construct comprising several genes, overview | Propionibacterium freudenreichii |
2.1.1.132 | genes cbiEGH and cbiT, the gene cbiE of Propionibacterium freudenreichii is organized in a single ORF with genes cbiH and cbiG, DNA and amino acid sequence determination and analysis, genetic organization, co-overexpression of enzymes and reconstruction of the anaerobic cobalamin biosynthetic pathway in Escherichia coli in a construct comprising several genes, overview | Propionibacterium freudenreichii |
2.1.1.271 | gene cbiF, DNA and amino acid sequence determination and analysis, genetic organization, co-overexpression of enzymes and reconstruction of the anaerobic cobalamin biosynthetic pathway in Escherichia coli in a construct comprising several genes, overview | Propionibacterium freudenreichii |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.1.1.130 | additional information | complementation with gene cbiL of Propionibacterium freudenreichii of 2 mutants AR3711 and AR3717 of an engineered Escherichia coli strain that synthesizes cobinamide using the Salmonella typhimurium cbi genes | Propionibacterium freudenreichii |
2.1.1.130 | additional information | complementation with gene cbiL of Propionibacterium freudenreichii of 2 mutants AR3711 and AR3717 of an engineered Escherichia coli strain that synthesizes cobinamide using the Salmonella typhimurium cbi genes, overview | Salmonella enterica subsp. enterica serovar Typhimurium |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.1.1.131 | 91400 | - |
x * 91400, about, cbiEGH protein, amino acid sequence calculation and SDS-PAGE | Propionibacterium freudenreichii |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.130 | additional information | Pseudomonas denitrificans (nom. rej.) | the organism performs the aerobic cobalamin biosynthesis variant | ? | - |
? | |
2.1.1.130 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium | the organism performs the anaerobic cobalamin biosynthesis variant | ? | - |
? | |
2.1.1.130 | additional information | Propionibacterium freudenreichii | the organism performs the anaerobic cobalamin biosynthesis variant | ? | - |
? | |
2.1.1.130 | S-adenosyl-L-methionine + cobalt-precorrin-2 | Salmonella enterica subsp. enterica serovar Typhimurium | enzyme is involved in anaerobic cobalamin, i.e. vitamin B12, biosynthesis, overview | S-adenosyl-L-homocysteine + cobalt-precorrin-3A | - |
? | |
2.1.1.130 | S-adenosyl-L-methionine + cobalt-precorrin-2 | Pseudomonas denitrificans (nom. rej.) | enzyme is involved in anaerobic cobalamin, i.e. vitamin B12, biosynthesis, pathway overview | S-adenosyl-L-homocysteine + cobalt-precorrin-3A | - |
? | |
2.1.1.130 | S-adenosyl-L-methionine + cobalt-precorrin-2 | Propionibacterium freudenreichii | enzyme is involved in anaerobic cobalamin, i.e. vitamin B12, biosynthesis, pathway overview | S-adenosyl-L-homocysteine + cobalt-precorrin-3A | - |
? | |
2.1.1.131 | additional information | Pseudomonas denitrificans (nom. rej.) | the organism performs the aerobic cobalamin biosynthesis variant | ? | - |
? | |
2.1.1.131 | additional information | Propionibacterium freudenreichii | the organism performs the anaerobic cobalamin biosynthesis variant, the gene cbiH of Propionibacterium freudenreichii is organized in a single ORF with genes cbiE and cbiG, overview | ? | - |
? | |
2.1.1.131 | S-adenosyl-L-methionine + cobalt-precorrin-3B | Pseudomonas denitrificans (nom. rej.) | enzyme is involved in the anaerobic cobalamin biosynthesis, pathway overview | S-adenosyl-L-homocysteine + cobalt-precorrin-4 | - |
? | |
2.1.1.131 | S-adenosyl-L-methionine + cobalt-precorrin-3B | Propionibacterium freudenreichii | enzyme is involved in the anaerobic cobalamin biosynthesis, pathway overview | S-adenosyl-L-homocysteine + cobalt-precorrin-4 | - |
? | |
2.1.1.132 | additional information | Pseudomonas denitrificans (nom. rej.) | the organism performs the aerobic cobalamin biosynthesis variant | ? | - |
? | |
2.1.1.132 | additional information | Propionibacterium freudenreichii | the organism performs the anaerobic cobalamin biosynthesis variant, the gene cbiE of Propionibacterium freudenreichii is organized in a single ORF with genes cbiH and cbiG, overview | ? | - |
? | |
2.1.1.132 | S-adenosyl-L-methionine + cobalt-precorrin-6Y + 6 H+ | Propionibacterium freudenreichii | methylation and decarboxylation are catalyzed by 2 different enzymes in Propionibacterium freudenreichii, encoded by gene cbiE, organized in a orf/single gene with cbiG and cbiH, which is responsible for the methylations at C5 and C15, and by gene cbiT, which is responsible for the decarboxylation, steps of the anaerobic cobalamin biosynthesis, pathway overview | S-adenosyl-L-homocysteine + cobalt-precorrin-8X + 2 H2O | - |
? | |
2.1.1.132 | S-adenosyl-L-methionine + cobalt-precorrin-6Y + 6 H+ | Pseudomonas denitrificans (nom. rej.) | steps of the aerobic cobalamin biosynthesis, pathway overview | S-adenosyl-L-homocysteine + cobalt-precorrin-8X + 2 H2O | - |
? | |
2.1.1.271 | additional information | Pseudomonas denitrificans (nom. rej.) | the organism performs the aerobic cobalamin biosynthesis variant | ? | - |
? | |
2.1.1.271 | additional information | Propionibacterium freudenreichii | the organism performs the anaerobic cobalamin biosynthesis variant | ? | - |
? | |
2.1.1.271 | S-adenosyl-L-methionine + cobalt-precorrin-4 | Propionibacterium freudenreichii | enzyme is involved in anaerobic cobalamin, i.e. vitamin B12, biosynthesis, pathway overview | S-adenosyl-L-homocysteine + cobalt-precorrin-5 | - |
? | |
2.1.1.271 | S-adenosyl-L-methionine + precorrin-4 | Pseudomonas denitrificans (nom. rej.) | enzyme is involved in aerobic cobalamin, i.e. vitamin B12, biosynthesis, pathway overview | S-adenosyl-L-homocysteine + precorrin-5 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.1.1.130 | Propionibacterium freudenreichii | - |
i.e. Propionibacterium shermanii, gene cbiL | - |
2.1.1.130 | Pseudomonas denitrificans (nom. rej.) | - |
gene cobI | - |
2.1.1.130 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene cbiL | - |
2.1.1.131 | Propionibacterium freudenreichii | - |
i.e. Propionibacterium shermanii, gene cbiH | - |
2.1.1.131 | Pseudomonas denitrificans (nom. rej.) | - |
gene cobJ | - |
2.1.1.132 | Propionibacterium freudenreichii | - |
i.e. Propionibacterium shermanii, gene cbiE | - |
2.1.1.132 | Pseudomonas denitrificans (nom. rej.) | - |
gene cobL | - |
2.1.1.271 | Propionibacterium freudenreichii | - |
i.e. Propionibacterium shermanii, gene cbiF | - |
2.1.1.271 | Pseudomonas denitrificans (nom. rej.) | - |
gene cobM | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.1.1.130 | additional information | the organism performs the aerobic cobalamin biosynthesis variant | Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
2.1.1.130 | additional information | the organism performs the anaerobic cobalamin biosynthesis variant | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
2.1.1.130 | additional information | the organism performs the anaerobic cobalamin biosynthesis variant | Propionibacterium freudenreichii | ? | - |
? | |
2.1.1.130 | S-adenosyl-L-methionine + cobalt-precorrin-2 | enzyme is involved in anaerobic cobalamin, i.e. vitamin B12, biosynthesis, overview | Salmonella enterica subsp. enterica serovar Typhimurium | S-adenosyl-L-homocysteine + cobalt-precorrin-3A | - |
? | |
2.1.1.130 | S-adenosyl-L-methionine + cobalt-precorrin-2 | enzyme is involved in anaerobic cobalamin, i.e. vitamin B12, biosynthesis, pathway overview | Pseudomonas denitrificans (nom. rej.) | S-adenosyl-L-homocysteine + cobalt-precorrin-3A | - |
? | |
2.1.1.130 | S-adenosyl-L-methionine + cobalt-precorrin-2 | enzyme is involved in anaerobic cobalamin, i.e. vitamin B12, biosynthesis, pathway overview | Propionibacterium freudenreichii | S-adenosyl-L-homocysteine + cobalt-precorrin-3A | - |
? | |
2.1.1.130 | S-adenosyl-L-methionine + cobalt-precorrin-2 | methylation at C-20 | Salmonella enterica subsp. enterica serovar Typhimurium | S-adenosyl-L-homocysteine + cobalt-precorrin-3A | - |
? | |
2.1.1.130 | S-adenosyl-L-methionine + cobalt-precorrin-2 | methylation at C-20 | Pseudomonas denitrificans (nom. rej.) | S-adenosyl-L-homocysteine + cobalt-precorrin-3A | - |
? | |
2.1.1.130 | S-adenosyl-L-methionine + cobalt-precorrin-2 | methylation at C-20 | Propionibacterium freudenreichii | S-adenosyl-L-homocysteine + cobalt-precorrin-3A | - |
? | |
2.1.1.131 | additional information | the organism performs the aerobic cobalamin biosynthesis variant | Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
2.1.1.131 | additional information | the organism performs the anaerobic cobalamin biosynthesis variant, the gene cbiH of Propionibacterium freudenreichii is organized in a single ORF with genes cbiE and cbiG, overview | Propionibacterium freudenreichii | ? | - |
? | |
2.1.1.131 | S-adenosyl-L-methionine + cobalt-precorrin-3 | C-17 methylation and ring contraction | Pseudomonas denitrificans (nom. rej.) | S-adenosyl-L-homocysteine + cobalt-precorrin-4 | - |
? | |
2.1.1.131 | S-adenosyl-L-methionine + cobalt-precorrin-3 | C-17 methylation and ring contraction | Propionibacterium freudenreichii | S-adenosyl-L-homocysteine + cobalt-precorrin-4 | - |
? | |
2.1.1.131 | S-adenosyl-L-methionine + cobalt-precorrin-3B | enzyme is involved in the anaerobic cobalamin biosynthesis, pathway overview | Pseudomonas denitrificans (nom. rej.) | S-adenosyl-L-homocysteine + cobalt-precorrin-4 | - |
? | |
2.1.1.131 | S-adenosyl-L-methionine + cobalt-precorrin-3B | enzyme is involved in the anaerobic cobalamin biosynthesis, pathway overview | Propionibacterium freudenreichii | S-adenosyl-L-homocysteine + cobalt-precorrin-4 | - |
? | |
2.1.1.132 | additional information | the organism performs the aerobic cobalamin biosynthesis variant | Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
2.1.1.132 | additional information | the organism performs the anaerobic cobalamin biosynthesis variant, the gene cbiE of Propionibacterium freudenreichii is organized in a single ORF with genes cbiH and cbiG, overview | Propionibacterium freudenreichii | ? | - |
? | |
2.1.1.132 | S-adenosyl-L-methionine + cobalt-precorrin-6Y + 6 H+ | methylation and decarboxylation are catalyzed by 2 different enzymes in Propionibacterium freudenreichii, encoded by gene cbiE, organized in a orf/single gene with cbiG and cbiH, which is responsible for the methylations at C5 and C15, and by gene cbiT, which is responsible for the decarboxylation, steps of the anaerobic cobalamin biosynthesis, pathway overview | Propionibacterium freudenreichii | S-adenosyl-L-homocysteine + cobalt-precorrin-8X + 2 H2O | - |
? | |
2.1.1.132 | S-adenosyl-L-methionine + cobalt-precorrin-6Y + 6 H+ | steps of the aerobic cobalamin biosynthesis, pathway overview | Pseudomonas denitrificans (nom. rej.) | S-adenosyl-L-homocysteine + cobalt-precorrin-8X + 2 H2O | - |
? | |
2.1.1.132 | S-adenosyl-L-methionine + cobalt-precorrin-6Y + 6 H+ | methylation and decarboxylation are catalyzed by 2 different enzymes in Propionibacterium freudenreichii, encoded by gene cbiE, organized in a orf/single gene with cbiG and cbiH, which is responsible for the methylations at C5 and C15, and by gene cbiT, which is responsible for the decarboxylation, overview | Propionibacterium freudenreichii | S-adenosyl-L-homocysteine + cobalt-precorrin-8X + 2 H2O | - |
? | |
2.1.1.132 | S-adenosyl-L-methionine + cobalt-precorrin-6Y + 6 H+ | methylation at C5 and C15, and decarboxylation | Pseudomonas denitrificans (nom. rej.) | S-adenosyl-L-homocysteine + cobalt-precorrin-8X + 2 H2O | - |
? | |
2.1.1.271 | additional information | the organism performs the aerobic cobalamin biosynthesis variant | Pseudomonas denitrificans (nom. rej.) | ? | - |
? | |
2.1.1.271 | additional information | the organism performs the anaerobic cobalamin biosynthesis variant | Propionibacterium freudenreichii | ? | - |
? | |
2.1.1.271 | S-adenosyl-L-methionine + cobalt-precorrin-4 | enzyme is involved in anaerobic cobalamin, i.e. vitamin B12, biosynthesis, pathway overview | Propionibacterium freudenreichii | S-adenosyl-L-homocysteine + cobalt-precorrin-5 | - |
? | |
2.1.1.271 | S-adenosyl-L-methionine + cobalt-precorrin-4 | C-11 methylation | Propionibacterium freudenreichii | S-adenosyl-L-homocysteine + cobalt-precorrin-5 | - |
? | |
2.1.1.271 | S-adenosyl-L-methionine + precorrin-4 | enzyme is involved in aerobic cobalamin, i.e. vitamin B12, biosynthesis, pathway overview | Pseudomonas denitrificans (nom. rej.) | S-adenosyl-L-homocysteine + precorrin-5 | - |
? | |
2.1.1.271 | S-adenosyl-L-methionine + precorrin-4 | C-11 methylation | Pseudomonas denitrificans (nom. rej.) | S-adenosyl-L-homocysteine + precorrin-5 | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.1.1.131 | ? | x * 91400, about, cbiEGH protein, amino acid sequence calculation and SDS-PAGE | Propionibacterium freudenreichii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.1.1.130 | S-adenosyl-L-methionine | - |
Salmonella enterica subsp. enterica serovar Typhimurium | |
2.1.1.130 | S-adenosyl-L-methionine | - |
Pseudomonas denitrificans (nom. rej.) | |
2.1.1.130 | S-adenosyl-L-methionine | - |
Propionibacterium freudenreichii |