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Literature summary extracted from

  • Hoober, K.L.; Thorpe, C.
    Flavin-dependent sulfhydryl oxidases in protein disulfide bond formation (2002), Methods Enzymol., 348, 30-34.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.3.2 0.014
-
 RNAse A pH 8.1, 25°C Gallus gallus
1.8.3.2 0.2
-
 DTT 7.5, 25°C Gallus gallus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.3.2 extracellular secretion of enzyme Homo sapiens
-
-
1.8.3.2 extracellular secretion of enzyme Aspergillus niger
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.8.3.2 additional information metalloenzyme Gallus gallus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.3.2 R-SH + O2 Gallus gallus
-
 R-S-S-R + H2O2
-
 ir
1.8.3.2 R-SH + O2 Aspergillus niger
-
 R-S-S-R + H2O2
-
 ir
1.8.3.2 R-SH + O2 Homo sapiens enzyme might counterbalance the plasmin reductase in extracellular reductive processes R-S-S-R + H2O2
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
1.8.3.2 Aspergillus niger
-
-
-
1.8.3.2 Gallus gallus
-
-
-
1.8.3.2 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.3.2 from egg white form a riboflavin-binding, protein-deficient strain, several steps Gallus gallus

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.3.2 2 R'C(R)SH + O2 = R'C(R)S-S(R)CR' + H2O2 enzyme contains a functionally involved redox-active motif CXXC Gallus gallus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.3.2 egg white
-
 Gallus gallus
-
1.8.3.2 fibroblast
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.3.2 additional information
-
 several assay methods are used dependent on the substrate, overview Gallus gallus

Storage Stability

EC Number Storage Stability Organism
1.8.3.2 -20°C, purified enzyme from egg white, in 20 mM Tris buffer, stable for months Gallus gallus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.3.2 dithiothreitol + O2
-
 Gallus gallus dithiothreitol disulfide + H2O2
-
 ir
1.8.3.2 additional information preferred substrates are protein or peptide sulfhydryl groups, but not low molecular weight thiols, such as cysteine or glutathione Gallus gallus ?
-
 ?
1.8.3.2 protein disulfide isomerase + O2 i.e. PDI Gallus gallus protein disulfide isomerase disulfide + H2O2
-
 ir
1.8.3.2 R-SH + O2
-
 Gallus gallus R-S-S-R + H2O2
-
 ir
1.8.3.2 R-SH + O2
-
 Aspergillus niger R-S-S-R + H2O2
-
 ir
1.8.3.2 R-SH + O2 enzyme might counterbalance the plasmin reductase in extracellular reductive processes Homo sapiens R-S-S-R + H2O2
-
 ir
1.8.3.2 R-SH + O2 oxidation of thiols to disulfides with a concomitant reduction of molecular oxygen to peroxide Gallus gallus R-S-S-R + H2O2
-
 ir
1.8.3.2 R-SH + O2 oxidation of thiols to disulfides with a concomitant reduction of molecular oxygen to peroxide Homo sapiens R-S-S-R + H2O2
-
 ir
1.8.3.2 R-SH + O2 oxidation of thiols to disulfides with a concomitant reduction of molecular oxygen to peroxide Aspergillus niger R-S-S-R + H2O2
-
 ir
1.8.3.2 RNase A + O2
-
 Gallus gallus RNase A disulfide + H2O2
-
 ir
1.8.3.2 thioredoxin + O2 substrate from Escherichia coli Gallus gallus thioredoxin disulfide + H2O2
-
 ir

Subunits

EC Number Subunits Comment Organism
1.8.3.2 More enzyme contains an N-terminal thioredoxin domain, an intervening domain, and a C-terminal ALR/ERV domain, redox-active motif CXXC Gallus gallus

Synonyms

EC Number Synonyms Comment Organism
1.8.3.2 egg white oxidase
-
 Gallus gallus
1.8.3.2 More enzyme belongs to the sulfhydryl oxidase/Quiescin Q6 family Gallus gallus
1.8.3.2 More enzyme belongs to the sulfhydryl oxidase/Quiescin Q6 family Homo sapiens
1.8.3.2 Quiescin Q6
-
 Homo sapiens
1.8.3.2 sulfhydryl oxidase
-
 Gallus gallus
1.8.3.2 sulfhydryl oxidase
-
 Homo sapiens
1.8.3.2 sulfhydryl oxidase
-
 Aspergillus niger

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.3.2 FAD dependent on Gallus gallus
1.8.3.2 FAD bound to the enzyme Homo sapiens
1.8.3.2 FAD bound to the enzyme Aspergillus niger
1.8.3.2 additional information enzyme contains a redox-active cysteine-bridge Gallus gallus
1.8.3.2 additional information enzyme contains a redox-active cysteine-bridge Homo sapiens
1.8.3.2 additional information enzyme contains a redox-active cysteine-bridge Aspergillus niger