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Literature summary extracted from

  • Bonner, C.; Jensen, R.
    Prephenate aminotransferase (1987), Methods Enzymol., 142, 479-487.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.79 pyridoxal 5'-phosphate, EDTA, and glycerol stabilize, required for activity assay Nicotiana sylvestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.79 prephenate substrate inhibition above 1 mM, 80% of maximal activity at 2 mM, 38% of maximal activity at 20 mM Nicotiana sylvestris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.79 88000
-
 gel filtration Nicotiana sylvestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.79 prephenate + L-glutamate [Brevibacterium] flavum
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Nicotiana sylvestris
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Magnolia sp.
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Sorghum sp.
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Spinacia oleracea
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Lemna sp.
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Hemerocallis hybrid cultivar
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Corynebacterium glutamicum
-
 L-arogenate + 2-oxoglutarate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.79 Corynebacterium glutamicum
-
-
-
2.6.1.79 Hemerocallis hybrid cultivar
-
 day lily
-
2.6.1.79 Lemna sp.
-
 duckweed
-
2.6.1.79 Magnolia sp.
-
-
-
2.6.1.79 Nicotiana sylvestris
-
-
-
2.6.1.79 Sorghum sp.
-
-
-
2.6.1.79 Spinacia oleracea
-
-
-
2.6.1.79 [Brevibacterium] flavum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.79 252fold from cell culture by ammonium sulfate fractionation, heat treatment, affinity chromatography, and gel filtration Nicotiana sylvestris
2.6.1.79 to homogeneity Corynebacterium glutamicum
2.6.1.79 to homogeneity [Brevibacterium] flavum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.79 cell culture
-
 [Brevibacterium] flavum
-
2.6.1.79 cell culture
-
 Nicotiana sylvestris
-
2.6.1.79 cell culture
-
 Sorghum sp.
-
2.6.1.79 cell culture
-
 Spinacia oleracea
-
2.6.1.79 cell culture
-
 Lemna sp.
-
2.6.1.79 cell culture
-
 Hemerocallis hybrid cultivar
-
2.6.1.79 cell culture
-
 Corynebacterium glutamicum
-
2.6.1.79 leaf
-
 [Brevibacterium] flavum
-
2.6.1.79 leaf
-
 Nicotiana sylvestris
-
2.6.1.79 leaf
-
 Magnolia sp.
-
2.6.1.79 leaf
-
 Sorghum sp.
-
2.6.1.79 leaf
-
 Spinacia oleracea
-
2.6.1.79 leaf
-
 Lemna sp.
-
2.6.1.79 leaf
-
 Hemerocallis hybrid cultivar
-
2.6.1.79 leaf
-
 Corynebacterium glutamicum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.79 additional information
-
 different assay methods using L-glutamate or L-aspartate, development of coupled assay methods, overview Nicotiana sylvestris
2.6.1.79 8.3
-
 purified enzyme Nicotiana sylvestris

Storage Stability

EC Number Storage Stability Organism
2.6.1.79 -80°C, purified enzyme is stable for at least 1 month, partially purified enzyme is stable for at least several months Nicotiana sylvestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 [Brevibacterium] flavum L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate about 10% of the activity with prephenate Nicotiana sylvestris L-tyrosine + 2-oxoglutarate
-
 r
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Magnolia sp. L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Sorghum sp. L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Spinacia oleracea L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Lemna sp. L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Hemerocallis hybrid cultivar L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Corynebacterium glutamicum L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 additional information the substrate specificity of the enzyme in bacteria is less high than in plants [Brevibacterium] flavum ?
-
 ?
2.6.1.79 additional information no activity with oxaloacetate, 2-ketoglutarate, or pyruvate with L-glutamate Nicotiana sylvestris ?
-
 ?
2.6.1.79 additional information the substrate specificity of the enzyme in bacteria is less high than in plants Corynebacterium glutamicum ?
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 [Brevibacterium] flavum L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate about 5% of the activity with prephenate Nicotiana sylvestris L-phenylalanine + 2-oxoglutarate
-
 r
2.6.1.79 phenylpyruvate + L-glutamate
-
 Magnolia sp. L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Sorghum sp. L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Spinacia oleracea L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Lemna sp. L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Hemerocallis hybrid cultivar L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Corynebacterium glutamicum L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 prephenate + L-aspartate about 50% of the activity with L-glutamate Nicotiana sylvestris L-arogenate + oxaloacetate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 [Brevibacterium] flavum L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Nicotiana sylvestris L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Magnolia sp. L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Sorghum sp. L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Spinacia oleracea L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Lemna sp. L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Hemerocallis hybrid cultivar L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Corynebacterium glutamicum L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate [Brevibacterium] flavum L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate, optimal at low concentrations of about 1 mM Nicotiana sylvestris L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Magnolia sp. L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Sorghum sp. L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Spinacia oleracea L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Lemna sp. L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Hemerocallis hybrid cultivar L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Corynebacterium glutamicum L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 pyruvate + L-glutamate about 20% of the activity with prephenate Nicotiana sylvestris L-alanine + 2-oxoglutarate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.79 prephenate aminotransferase
-
 [Brevibacterium] flavum
2.6.1.79 prephenate aminotransferase
-
 Nicotiana sylvestris
2.6.1.79 prephenate aminotransferase
-
 Magnolia sp.
2.6.1.79 prephenate aminotransferase
-
 Sorghum sp.
2.6.1.79 prephenate aminotransferase
-
 Spinacia oleracea
2.6.1.79 prephenate aminotransferase
-
 Lemna sp.
2.6.1.79 prephenate aminotransferase
-
 Hemerocallis hybrid cultivar
2.6.1.79 prephenate aminotransferase
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.79 37
-
 assay at Nicotiana sylvestris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.79 25 70
-
 Nicotiana sylvestris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.79 70
-
 stable up to [Brevibacterium] flavum
2.6.1.79 70
-
 stable up to, 30 min Nicotiana sylvestris
2.6.1.79 70
-
 stable up to Magnolia sp.
2.6.1.79 70
-
 stable up to Sorghum sp.
2.6.1.79 70
-
 stable up to Spinacia oleracea
2.6.1.79 70
-
 stable up to Lemna sp.
2.6.1.79 70
-
 stable up to Hemerocallis hybrid cultivar
2.6.1.79 70
-
 stable up to Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.79 8 8.6
-
 Nicotiana sylvestris

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.79 pyridoxal 5'-phosphate
-
 [Brevibacterium] flavum
2.6.1.79 pyridoxal 5'-phosphate tightly bound to the enzyme Nicotiana sylvestris
2.6.1.79 pyridoxal 5'-phosphate
-
 Magnolia sp.
2.6.1.79 pyridoxal 5'-phosphate
-
 Sorghum sp.
2.6.1.79 pyridoxal 5'-phosphate
-
 Spinacia oleracea
2.6.1.79 pyridoxal 5'-phosphate
-
 Lemna sp.
2.6.1.79 pyridoxal 5'-phosphate
-
 Hemerocallis hybrid cultivar
2.6.1.79 pyridoxal 5'-phosphate
-
 Corynebacterium glutamicum