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Literature summary extracted from

  • Bonner, C.; Jensen, R.
    Prephenate aminotransferase (1987), Methods Enzymol., 142, 479-487.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.6.1.79 pyridoxal 5'-phosphate, EDTA, and glycerol stabilize, required for activity assay Nicotiana sylvestris

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.79 prephenate substrate inhibition above 1 mM, 80% of maximal activity at 2 mM, 38% of maximal activity at 20 mM Nicotiana sylvestris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.79 88000
-
 gel filtration Nicotiana sylvestris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.79 prephenate + L-glutamate Spinacia oleracea
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate [Brevibacterium] flavum
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Corynebacterium glutamicum
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Nicotiana sylvestris
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Sorghum sp.
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Magnolia sp.
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Lemna sp.
-
 L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate Hemerocallis hybrid cultivar
-
 L-arogenate + 2-oxoglutarate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.79 Corynebacterium glutamicum
-
-
-
2.6.1.79 Hemerocallis hybrid cultivar
-
 day lily
-
2.6.1.79 Lemna sp.
-
 duckweed
-
2.6.1.79 Magnolia sp.
-
-
-
2.6.1.79 Nicotiana sylvestris
-
-
-
2.6.1.79 Sorghum sp.
-
-
-
2.6.1.79 Spinacia oleracea
-
-
-
2.6.1.79 [Brevibacterium] flavum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.79 252fold from cell culture by ammonium sulfate fractionation, heat treatment, affinity chromatography, and gel filtration Nicotiana sylvestris
2.6.1.79 to homogeneity [Brevibacterium] flavum
2.6.1.79 to homogeneity Corynebacterium glutamicum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.6.1.79 cell culture
-
 Spinacia oleracea
-
2.6.1.79 cell culture
-
 [Brevibacterium] flavum
-
2.6.1.79 cell culture
-
 Corynebacterium glutamicum
-
2.6.1.79 cell culture
-
 Nicotiana sylvestris
-
2.6.1.79 cell culture
-
 Sorghum sp.
-
2.6.1.79 cell culture
-
 Lemna sp.
-
2.6.1.79 cell culture
-
 Hemerocallis hybrid cultivar
-
2.6.1.79 leaf
-
 Spinacia oleracea
-
2.6.1.79 leaf
-
 [Brevibacterium] flavum
-
2.6.1.79 leaf
-
 Corynebacterium glutamicum
-
2.6.1.79 leaf
-
 Nicotiana sylvestris
-
2.6.1.79 leaf
-
 Sorghum sp.
-
2.6.1.79 leaf
-
 Magnolia sp.
-
2.6.1.79 leaf
-
 Lemna sp.
-
2.6.1.79 leaf
-
 Hemerocallis hybrid cultivar
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.79 additional information
-
 different assay methods using L-glutamate or L-aspartate, development of coupled assay methods, overview Nicotiana sylvestris
2.6.1.79 8.3
-
 purified enzyme Nicotiana sylvestris

Storage Stability

EC Number Storage Stability Organism
2.6.1.79 -80°C, purified enzyme is stable for at least 1 month, partially purified enzyme is stable for at least several months Nicotiana sylvestris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Spinacia oleracea L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 [Brevibacterium] flavum L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Corynebacterium glutamicum L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Sorghum sp. L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Magnolia sp. L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Lemna sp. L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate
-
 Hemerocallis hybrid cultivar L-tyrosine + 2-oxoglutarate
-
 ?
2.6.1.79 4-hydroxyphenylpyruvate + L-glutamate about 10% of the activity with prephenate Nicotiana sylvestris L-tyrosine + 2-oxoglutarate
-
 r
2.6.1.79 additional information no activity with oxaloacetate, 2-ketoglutarate, or pyruvate with L-glutamate Nicotiana sylvestris ?
-
 ?
2.6.1.79 additional information the substrate specificity of the enzyme in bacteria is less high than in plants [Brevibacterium] flavum ?
-
 ?
2.6.1.79 additional information the substrate specificity of the enzyme in bacteria is less high than in plants Corynebacterium glutamicum ?
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Spinacia oleracea L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 [Brevibacterium] flavum L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Corynebacterium glutamicum L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Sorghum sp. L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Magnolia sp. L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Lemna sp. L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate
-
 Hemerocallis hybrid cultivar L-phenylalanine + 2-oxoglutarate
-
 ?
2.6.1.79 phenylpyruvate + L-glutamate about 5% of the activity with prephenate Nicotiana sylvestris L-phenylalanine + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-aspartate about 50% of the activity with L-glutamate Nicotiana sylvestris L-arogenate + oxaloacetate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Spinacia oleracea L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 [Brevibacterium] flavum L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Corynebacterium glutamicum L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Nicotiana sylvestris L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Sorghum sp. L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Magnolia sp. L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Lemna sp. L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate
-
 Hemerocallis hybrid cultivar L-arogenate + 2-oxoglutarate
-
 r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Spinacia oleracea L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate [Brevibacterium] flavum L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Corynebacterium glutamicum L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Sorghum sp. L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Magnolia sp. L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Lemna sp. L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate Hemerocallis hybrid cultivar L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 prephenate + L-glutamate prephenate is the preferred substrate, optimal at low concentrations of about 1 mM Nicotiana sylvestris L-arogenate + 2-oxoglutarate L-arogenate is cyclohexadienylic acid r
2.6.1.79 pyruvate + L-glutamate about 20% of the activity with prephenate Nicotiana sylvestris L-alanine + 2-oxoglutarate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.79 prephenate aminotransferase
-
 Spinacia oleracea
2.6.1.79 prephenate aminotransferase
-
 [Brevibacterium] flavum
2.6.1.79 prephenate aminotransferase
-
 Corynebacterium glutamicum
2.6.1.79 prephenate aminotransferase
-
 Nicotiana sylvestris
2.6.1.79 prephenate aminotransferase
-
 Sorghum sp.
2.6.1.79 prephenate aminotransferase
-
 Magnolia sp.
2.6.1.79 prephenate aminotransferase
-
 Lemna sp.
2.6.1.79 prephenate aminotransferase
-
 Hemerocallis hybrid cultivar

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.79 37
-
 assay at Nicotiana sylvestris

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.79 25 70
-
 Nicotiana sylvestris

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.6.1.79 70
-
 stable up to Spinacia oleracea
2.6.1.79 70
-
 stable up to [Brevibacterium] flavum
2.6.1.79 70
-
 stable up to Corynebacterium glutamicum
2.6.1.79 70
-
 stable up to Sorghum sp.
2.6.1.79 70
-
 stable up to Magnolia sp.
2.6.1.79 70
-
 stable up to Lemna sp.
2.6.1.79 70
-
 stable up to Hemerocallis hybrid cultivar
2.6.1.79 70
-
 stable up to, 30 min Nicotiana sylvestris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.79 8 8.6
-
 Nicotiana sylvestris

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.79 pyridoxal 5'-phosphate
-
 Spinacia oleracea
2.6.1.79 pyridoxal 5'-phosphate
-
 [Brevibacterium] flavum
2.6.1.79 pyridoxal 5'-phosphate
-
 Corynebacterium glutamicum
2.6.1.79 pyridoxal 5'-phosphate
-
 Sorghum sp.
2.6.1.79 pyridoxal 5'-phosphate
-
 Magnolia sp.
2.6.1.79 pyridoxal 5'-phosphate
-
 Lemna sp.
2.6.1.79 pyridoxal 5'-phosphate
-
 Hemerocallis hybrid cultivar
2.6.1.79 pyridoxal 5'-phosphate tightly bound to the enzyme Nicotiana sylvestris