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Literature summary extracted from

  • Wang, H.Y.; Li, Y.F.; Xie, L.X.; Xu, P.
    Expression of a bacterial aroA mutant, aroA-M1, encoding 5-enolpyruvylshikimate-3-phosphate synthase for the production of glyphosate-resistant tobacco plants (2003), J. Plant Res., 116, 455-460.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.19 expression of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium in Escherichia coli strain XL1-Blue Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 expression of the wild-type enzyme and of the chimeric mutant aroA-M1, comprising N-terminal part of the Escherichia coli enzyme and the C-terminal part of the enzyme from Salmonella typhimurium in strain XL1-Blue Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.19 additional information construction of chimeric mutants by fusing the N-terminal part of the Escherichia coli enzyme with the C-terminal part of the enzyme from Salmonella typhimurium, i.e. mutant construct aroA-M1, the chimeric mutant shows a 6fold lower Km for phosphoenolpyruvate, a 5fold higher Ki for glyphosate, and a 9fold higher specific activity than the wild-type enzyme from Escherichia coli, construction of glyphosate-resistant transgenic Nicotiana tabacum plants expressing the chimeric mutant aroA-M1 using the Agrobacterium tumefaciens strain LBA 4404 infection system Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 additional information construction of chimeric mutants by fusing the N-terminal part of the Escherichia coli enzyme with the C-terminal part of the enzyme from Salmonella typhimurium, the chimeric mutant shows a 6fold lower Km for phosphoenolpyruvate, a 5fold higher Ki for glyphosate, and a 9fold higher specific activity than the wild-type enzyme from Escherichia coli, construction of glyphosate-resistant transgenic Nicotiana tabacum plants expressing the chimeric mutant aroA-M1 using the Agrobacterium tumefaciens strain LBA 4404 infection system Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Salmonella enterica subsp. enterica serovar Typhimurium key enzyme in the shikimate pathway phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate Escherichia coli key enzyme in the shikimate pathway phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.19 Escherichia coli
-
 gene aroA
-
2.5.1.19 Salmonella enterica subsp. enterica serovar Typhimurium
-
 gene aroA
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Salmonella enterica subsp. enterica serovar Typhimurium phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate
-
 Escherichia coli phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate key enzyme in the shikimate pathway Salmonella enterica subsp. enterica serovar Typhimurium phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?
2.5.1.19 phosphoenolpyruvate + 3-phosphoshikimate key enzyme in the shikimate pathway Escherichia coli phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.19 5-enolpyruvylshikimate-3-phosphate synthase
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 5-enolpyruvylshikimate-3-phosphate synthase
-
 Escherichia coli
2.5.1.19 EPSPS
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.5.1.19 EPSPS
-
 Escherichia coli