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Literature summary extracted from

  • Takei, K.; Dekishima, Y.; Eguchi, T.; Yamaya, T.; Sakakibara, H.
    A new method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling (2003), J. Plant Res., 116, 259-263.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.5.1.27 synthesis method for enzymatic preparation of isopentenyladenine-type and trans-zeatin-type cytokinins with radioisotope-labeling. The method is based on EC 2.5.1.27 from Arabidopsis thaliana, EC 3.1.3.1 from calf intestine and EC 2.4.2.1 from Escherichia coli Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.27 expression in Escherichia coli Arabidopsis thaliana
2.5.1.112 expressed in Escherichia coli M15 cells Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.112 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP Arabidopsis thaliana
-
trans-zeatin riboside 5'-diphosphate + diphosphate
-
?
2.5.1.112 dimethylallyl diphosphate + ADP Arabidopsis thaliana
-
diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.27 Arabidopsis thaliana
-
-
-
2.5.1.112 Arabidopsis thaliana
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.27 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + AMP
-
Arabidopsis thaliana diphosphate + N6-(4-hydroxy-3-methyl-2-(E)-butyl)adenosine 5'-phosphate
-
?
2.5.1.27 dimethylallyl diphosphate + AMP
-
Arabidopsis thaliana diphosphate + N6-(dimethylallyl)adenosine 5'-phosphate
-
?
2.5.1.112 4-hydroxy-3-methyl-2-(E)-butenyl diphosphate + ADP
-
Arabidopsis thaliana trans-zeatin riboside 5'-diphosphate + diphosphate
-
?
2.5.1.112 dimethylallyl diphosphate + ADP
-
Arabidopsis thaliana diphosphate + N6-(dimethylallyl)adenosine 5'-diphosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.5.1.27 AtIPT7
-
Arabidopsis thaliana
2.5.1.112 adenylate isopentenyltransferase
-
Arabidopsis thaliana
2.5.1.112 IPT
-
Arabidopsis thaliana
2.5.1.112 IPT7
-
Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.5.1.112 ADP
-
Arabidopsis thaliana