Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Fischer, M.; Schott, A.K.; Romisch, W.; Ramsperger, A.; Augustin, M.; Fidler, A.; Bacher, A.; Richter, G.; Huber, R.; Eisenreich, W.
    Evolution of vitamin B2 biosynthesis. A novel class of riboflavin synthase in Archaea (2004), J. Mol. Biol., 343, 267-278.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.9 optimization of expression in Escherichia coli by construction of a synthetic gene Methanocaldococcus jannaschii
2.5.1.9 optimization of expression in Escherichia coli by construction of a synthetic gene, phylogenetic analysis Methanocaldococcus jannaschii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.9 Mg2+ preferred cation Methanocaldococcus jannaschii
2.5.1.9 Mn2+ preferred cation Methanocaldococcus jannaschii
2.5.1.9 additional information divalent cations are required for maximal activity Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.9 17500
-
 5 * 17500, recombinant enzyme Methanocaldococcus jannaschii
2.5.1.9 88300
-
 sedimentation equilibrium centrifugation, recombinant enzyme Methanocaldococcus jannaschii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-(1-D-ribityl)lumazine Methanocaldococcus jannaschii ultimate step in the biosynthesis of riboflavin, pathway overview, evolution of vitamin B2 biosynthesis riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.9 Methanocaldococcus jannaschii
-
 AY442958, GenBank synthetic gene
-
2.5.1.9 Methanocaldococcus jannaschii Q58584
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.9 recombinant enzyme from Escherichia coli to homogeneity Methanocaldococcus jannaschii

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.9 0.002
-
 purified recombinant enzyme, in absence of divalent cations Methanocaldococcus jannaschii
2.5.1.9 0.024
-
 purified recombinant enzyme, in presence of divalent cations Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.9 6,7-dimethyl-8-(1-D-ribityl)lumazine ultimate step in the biosynthesis of riboflavin, pathway overview, evolution of vitamin B2 biosynthesis Methanocaldococcus jannaschii riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
2.5.1.9 6,7-dimethyl-8-(1-D-ribityl)lumazine transfer of a 4-carbon fragment Methanocaldococcus jannaschii riboflavin + 5-amino-6-ribitylamino-2,4-(1H,3H)-pyrimidinedione
-
 ?
2.5.1.9 additional information activity with different isotopomers, stereochemistry, overview Methanocaldococcus jannaschii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.9 pentamer 5 * 17500, recombinant enzyme Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.9 40
-
 assay at Methanocaldococcus jannaschii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.9 7
-
 assay at Methanocaldococcus jannaschii