Literature summary extracted from
Searls, T.; Butler, D.; Chien, W.; Mukherji, M.; Lloyd, M.D.; Schofield, C.J.
Studies on the specificity of unprocessed and mature forms of phytanoyl-CoA 2-hydroxylase and mutation of the iron binding ligands (2005), J. Lipid Res., 46, 1660-1667.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
1.14.11.18 |
ATP |
at 0.1 mM Fe2+ 4 mM ATP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM ATP for 1.0 mM Fe2+ |
Homo sapiens |
|
1.14.11.18 |
GTP |
at 0.1 mM Fe2+ 4 mM GTP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM GTP for 1.0 mM Fe2+ |
Homo sapiens |
|
1.14.11.18 |
imidazole |
at 0.1 mM Fe2+ 4 mM ATP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM ATP for 1.0 mM Fe2+ |
Homo sapiens |
|
1.14.11.18 |
imidazole |
stimulates at 1.0 mM Fe2+ |
Homo sapiens |
|
Protein Variants
EC Number |
Protein Variants |
Comment |
Organism |
---|
1.14.11.18 |
D177A |
no activity with phytanoyl-CoA |
Homo sapiens |
1.14.11.18 |
H175A |
no activity with phytanoyl-CoA |
Homo sapiens |
1.14.11.18 |
H213A |
insoluble mutant enzyme |
Homo sapiens |
1.14.11.18 |
H264A |
activity with phytanoyl-CoA is 7.5 of the wild-type activity |
Homo sapiens |
1.14.11.18 |
Q176A |
activity with phytanoyl-CoA is 16.3% of the wild-type activity |
Homo sapiens |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.14.11.18 |
ATP |
at 0.1 mM Fe2+ 4 mM ATP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM ATP for 1.0 mM Fe2+ |
Homo sapiens |
|
1.14.11.18 |
GTP |
at 0.1 mM Fe2+ 4 mM ATP inhibits enzyme activity. At increased Fe2+ concentrations ATP stimulates with a maximum at 64 mM ATP for 1.0 mM Fe2+ |
Homo sapiens |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.14.11.18 |
peroxisome |
- |
Homo sapiens |
5777 |
- |
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
1.14.11.18 |
Iron |
Fe2+ required. Maximal activity is 0.05-0.1 mM Fe2+ in absence of ATP |
Homo sapiens |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.14.11.18 |
Homo sapiens |
O14832 |
- |
- |
Posttranslational Modification
EC Number |
Posttranslational Modification |
Comment |
Organism |
---|
1.14.11.18 |
proteolytic modification |
unprocessed proenzyme contains a N-terminal peroxisomal targeting sequence that is cleaved to give mature PAHX. Both forms are able to hydroxylate a range of CoA derivatives, but under the same assay conditions, the N-terminal Hexa-His-tagged unprocessed form is less active than the nontagged mature form |
Homo sapiens |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.14.11.18 |
- |
Homo sapiens |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.14.11.18 |
PAHX |
- |
Homo sapiens |