EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.10 | dithiothreitol | 2.5 mM, increases activity 2fold | Mycobacterium tuberculosis | |
2.5.1.10 | Triton X-100 | 0.2%, 1.5fold increase in activity | Mycobacterium tuberculosis |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.10 | expression in Mycobacterium smegmatis using a new mycobacterial expression vector that encodes an in-frame N-terminal affinity tag fusion with the protein of interest | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.10 | Triton X-100 | above 0.2% | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.10 | 0.0098 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.10 | 0.043 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.5.1.10 | Fe2+ | absolutely dependent on presence of divalent cation. Supports activity to a lower level than Mg2+ | Mycobacterium tuberculosis | |
2.5.1.10 | Mg2+ | absolutely dependent on presence of divalent cation. Optimal activity at 4-10 mM | Mycobacterium tuberculosis | |
2.5.1.10 | Mn2+ | absolutely dependent on presence of divalent cation. Supports activity to a lower level than Mg2+ | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.10 | Mycobacterium tuberculosis | - |
- |
- |
2.5.1.10 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.5.1.10 | fusion protein | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.10 | geranyl diphosphate + isopentenyl diphosphate | no activity with dimethylallyl diphosphate | Mycobacterium tuberculosis | diphosphate + trans,trans-farnesyl diphosphate | - |
? | |
2.5.1.10 | geranyl diphosphate + isopentenyl diphosphate | no activity with dimethylallyl diphosphate | Mycobacterium tuberculosis H37Rv | diphosphate + trans,trans-farnesyl diphosphate | - |
? | |
2.5.1.10 | additional information | no activity with dimethylallyl diphosphate | Mycobacterium tuberculosis | ? | - |
? | |
2.5.1.10 | additional information | no activity with dimethylallyl diphosphate | Mycobacterium tuberculosis H37Rv | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.5.1.10 | omega,E,E-farnesyl diphosphate synthase | - |
Mycobacterium tuberculosis |
2.5.1.10 | omega,E,E-FPP | - |
Mycobacterium tuberculosis |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.5.1.10 | 0.2 | - |
geranyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis | |
2.5.1.10 | 0.49 | - |
isopentenyl diphosphate | pH 7.9, 37°C | Mycobacterium tuberculosis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.10 | 8 | - |
- |
Mycobacterium tuberculosis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
2.5.1.10 | 7 | 10 | active over the range pH 7-10 | Mycobacterium tuberculosis |