EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.2 | additional information | high enzyme yield when the bacterium is grown on peptone as nitrogen source, and on mannitol or glycerol as carbon source, overview | Gluconobacter oxydans |
EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.1.2 | synthesis | enzyme is utilized for bioconversions of glycosides in diverse purposes, overview, and for production of dextran and oligodextran, no product inhibition, enzyme is robust in high level production | Gluconobacter oxydans |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.2 | Cd2+ | partial inhibition at 1 mM | Gluconobacter oxydans | |
2.4.1.2 | Cu2+ | partial inhibition at 1 mM | Gluconobacter oxydans | |
2.4.1.2 | Hg2+ | complete inactivation at 1 mM | Gluconobacter oxydans | |
2.4.1.2 | KMnO4 | complete inactivation at 1 mM | Gluconobacter oxydans | |
2.4.1.2 | Pb2+ | complete inactivation at 1 mM | Gluconobacter oxydans | |
2.4.1.2 | Zn2+ | partial inhibition at 1 mM | Gluconobacter oxydans |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.2 | cell wall | - |
Gluconobacter oxydans | 5618 | - |
2.4.1.2 | cytoplasm | - |
Gluconobacter oxydans | 5737 | - |
2.4.1.2 | extracellular | cell wall bound | Gluconobacter oxydans | - |
- |
2.4.1.2 | intracellular | cytoplasmic | Gluconobacter oxydans | 5622 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.2 | additional information | Gluconobacter oxydans | substrate specificity, no activity with glucose or maltose, conversion of maltodextrins into (oligo)dextran forming an alpha1,6-linkage, consecutive glucosyl transfer results in formation of high molecular weight dextran, dextran synthesis pathway overview | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.2 | Gluconobacter oxydans | - |
strain ATCC 11894, extra- and intracellular enzyme variants | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.2 | extra- and intracellular enzymes | Gluconobacter oxydans |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.4.1.2 | [(1->4)-alpha-D-glucosyl]n + [(1->6)-alpha-D-glucosyl]m = [(1->4)-alpha-D-glucosyl]n-1 + [(1->6)-alpha-D-glucosyl]m+1 | reaction mode and mechanism | Gluconobacter oxydans |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.2 | additional information | high enzyme yield when the bacterium is grown on peptone as nitrogen source, and on mannitol or glycerol as carbon source, overview | Gluconobacter oxydans | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.2 | cellobiose + starch | starch is the donor substrate, cellobiose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | D-glucosyl-alpha-(1,4)-D-xylose + starch | starch is the donor substrate, D-glucosyl-alpha(1,4)-D-xylose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | dextran + salicin | dextran is the donor substrate, salicin the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | gentiobiose + starch | starch is the donor substrate, gentiobiose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | isomaltose + salicin | isomaltose is the donor substrate, salicin the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | isomaltose + starch | starch is the donor substrate, isomaltose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | isoprimeverose + starch | starch is the donor substrate, isoprimeverose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | kojibiose + starch | starch is the donor substrate, kojibiose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | laminaribiose + starch | starch is the donor substrate, laminaribiose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | maltose + salicin | maltose is the donor substrate, salicin the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | maltose + starch | starch is the donor substrate, maltose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | methyl-alpha-D-glucoside + starch | starch is the donor substrate, methyl-alpha-D-glucoside the acceptor substrate | Gluconobacter oxydans | methyl-alpha-isomaltoside + ? | - |
? | |
2.4.1.2 | methyl-beta-D-glucoside + starch | starch is the donor substrate, methyl-beta-D-glucoside the acceptor substrate | Gluconobacter oxydans | methyl-beta-isomaltoside + ? | - |
? | |
2.4.1.2 | additional information | substrate specificity, no activity with glucose or maltose, conversion of maltodextrins into (oligo)dextran forming an alpha1,6-linkage, consecutive glucosyl transfer results in formation of high molecular weight dextran, dextran synthesis pathway overview | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | additional information | donor and acceptor substrate specificity, no activity with trehalose, lactose, or melibiose as acceptor substrates, when starch is the donor substrate, overview, conversion of maltodextrins into (oligo)dextran forming an alpha1,6-linkage or an alpha1,4-linkage | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | nigerose + starch | starch is the donor substrate, nigerose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | salicin + starch | starch is the donor substrate, salicin, i.e. 2-(hydroxymethyl)phenyl-beta-D-glucopyranoside, the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | sophorose + starch | starch is the donor substrate, sophorose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | starch + salicin | starch is the donor substrate, salicin the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | sucrose + starch | starch is the donor substrate, sucrose the acceptor substrate | Gluconobacter oxydans | ? | - |
? | |
2.4.1.2 | xylosucrose + starch | starch is the donor substrate, xylosucrose the acceptor substrate | Gluconobacter oxydans | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.4.1.2 | DDase | - |
Gluconobacter oxydans |
2.4.1.2 | dextran dextrinase | - |
Gluconobacter oxydans |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.2 | 38 | - |
- |
Gluconobacter oxydans |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.2 | 45 | - |
purified extracellular enzyme, stable up to | Gluconobacter oxydans |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.2 | 5.2 | - |
- |
Gluconobacter oxydans |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.2 | 4.1 | 5.4 | purified extracellular enzyme, stable for 24 h at 4°C | Gluconobacter oxydans |