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Literature summary extracted from

  • Römer, U.; Schrader, H.; Gunther, N.; Nettelstroth, N.; Frommer, W.B.; Elling, L.
    Expression, purification and characterization of recombinant sucrose synthase 1 from Solanum tuberosum L. for carbohydrate engineering (2004), J. Biotechnol., 107, 135-149.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.13 constitutive expression in Saccharomyces cerevisiae strain 22574dsus1 Solanum tuberosum

General Stability

EC Number General Stability Organism
2.4.1.13 no activity is lost during 3 freeze-thaw-cycles Solanum tuberosum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.13 2,5-dihydroxymethyl-(3S,4R)-dihydroxypyrrolidine
-
 Solanum tuberosum
2.4.1.13 5-deoxy-beta-D-fructose competitive to D-fructose Solanum tuberosum
2.4.1.13 Co2+ 0.05 mM, 20% loss of activity Solanum tuberosum
2.4.1.13 Cu2+
-
 Solanum tuberosum
2.4.1.13 D-fructose
-
 Solanum tuberosum
2.4.1.13 Ni2+
-
 Solanum tuberosum
2.4.1.13 sucrose
-
 Solanum tuberosum
2.4.1.13 UDP
-
 Solanum tuberosum
2.4.1.13 UDP-glucose
-
 Solanum tuberosum
2.4.1.13 Zn2+
-
 Solanum tuberosum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.13 0.0041
-
 UDP pH 7.6, 30°C Solanum tuberosum
2.4.1.13 0.012
-
 dTDP pH 7.6, 30°C Solanum tuberosum
2.4.1.13 0.0916
-
 sucrose pH 7.6, 30°C Solanum tuberosum
2.4.1.13 0.5
-
 UDP-glucose pH 8.0, 30°C Solanum tuberosum
2.4.1.13 0.77
-
 UDP-GlcNAc pH 8.0, 30°C Solanum tuberosum
2.4.1.13 2.1
-
 D-fructose pH 8.0, 30°C Solanum tuberosum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.13 Ca2+ 20 mM, 2.5fold stimulation Solanum tuberosum
2.4.1.13 Mg2+ 20 mM, 3fold stimulation Solanum tuberosum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.13 90300
-
 4 * 90300, SDS-PAGE Solanum tuberosum
2.4.1.13 394000
-
 gel filtration Solanum tuberosum

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.13 Solanum tuberosum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.13
-
 Solanum tuberosum

Storage Stability

EC Number Storage Stability Organism
2.4.1.13 -20°C, pH 7.5, stored for at least 6 months with 10% loss of activity Solanum tuberosum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.13 TDP-glucose + D-fructose
-
 Solanum tuberosum TDP + sucrose
-
 ?
2.4.1.13 UDP + sucrose
-
 Solanum tuberosum UDP-glucose + D-fructose
-
 ?
2.4.1.13 UDP-N-acetyl-alpha-D-glucosamine + D-fructose
-
 Solanum tuberosum UDP + 2-acetamido-2-deoxy-D-glucopyranosyl-beta-D-fructofuranoside
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.13 tetramer 4 * 90300, SDS-PAGE Solanum tuberosum

Synonyms

EC Number Synonyms Comment Organism
2.4.1.13 sucrose synthase 1
-
 Solanum tuberosum
2.4.1.13 SuSy1
-
 Solanum tuberosum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.13 56
-
-
 Solanum tuberosum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.13 30
-
 pH 7.6, 200 mM HEPES-NaOH buffer, 48 h, 20% loss of activity Solanum tuberosum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.13 7.6
-
 cleavage of sucrose Solanum tuberosum
2.4.1.13 8
-
 synthesis of sucrose Solanum tuberosum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.4.1.13 7.5
-
 -20°C, 6 months, 10% loss of activity Solanum tuberosum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.13 0.015
-
 Cu2+
-
 Solanum tuberosum
2.4.1.13 0.025
-
 Zn2+
-
 Solanum tuberosum
2.4.1.13 0.035
-
 Ni2+
-
 Solanum tuberosum
2.4.1.13 0.11
-
 UDP
-
 Solanum tuberosum
2.4.1.13 0.3
-
 5-deoxy-beta-D-fructose
-
 Solanum tuberosum
2.4.1.13 2.3
-
 UDP-glucose
-
 Solanum tuberosum
2.4.1.13 35.9
-
 D-fructose
-
 Solanum tuberosum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.13 Solanum tuberosum
-
-
 5.5