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Literature summary extracted from

  • Timoteo, C.G.; Tavares, P.; Goodhew, C.F.; Duarte, L.C.; Jumel, K.; Girio, F.M.; Harding, S.; Pettigrew, G.W.; Moura, I.
    Ca2+ and the bacterial peroxidases: the cytochrome c peroxidase from Pseudomonas stutzeri (2003), J. Biol. Inorg. Chem., 8, 29-37.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.5 Ca2+ 1 mM of the cation in the assay solution inhibits the oxidation of horse cytochrome c but not Pseudomonas stutzeri cytochrome c551 Pseudomonas stutzeri

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.5 Ca2+ pre-incubation with the cation has no effect on activity Pseudomonas stutzeri
1.11.1.5 additional information catalytic center activity increases with ionic strength in the case of cytochrome c551, with horse heart cytochrome c, the catalytic center activity decreases exponentially Pseudomonas stutzeri

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.5 51000
-
 sedimentation velocity experiments, in the presence of EGTA Pseudomonas stutzeri
1.11.1.5 67000
-
 sedimentation velocity experiments, untreated enzyme Pseudomonas stutzeri
1.11.1.5 80000
-
 sedimentation velocity experiments, in the presence of Ca2+ Pseudomonas stutzeri
1.11.1.5 87000
-
 sedimentation velocity experiments, at higher protein concentrations Pseudomonas stutzeri

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.11.1.5 ferrocytochrome c + H2O2 Pseudomonas stutzeri
-
 ferricytochrome c + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.5 Pseudomonas stutzeri
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.5 gel filtration and ion exchange chromatography Pseudomonas stutzeri

Reaction

EC Number Reaction Comment Organism Reaction ID
1.11.1.5 2 ferrocytochrome c + H2O2 + 2 H+ = 2 ferricytochrome c + 2 H2O pseudo-first-order kinetics Pseudomonas stutzeri
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.5 ferrocytochrome c + H2O2
-
 Pseudomonas stutzeri ferricytochrome c + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.5 dimer ultracentrifugation, untreated enzyme Pseudomonas stutzeri
1.11.1.5 More in the presence of added Ca2+ or higher protein concentrations, the enzyme partially shifts to a higher state of aggregation, presumably tetramer Pseudomonas stutzeri