Literature summary extracted from
Shin, D.H.; Oganesyan, N.; Jancarik, J.; Yokota, H.; Kim, R.; Kim, S.H.
Crystal structure of a nicotinate phosphoribosyltransferase from Thermoplasma acidophilum (2005), J. Biol. Chem., 280, 18326-18335.
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
6.3.4.21 |
expression of the enzyme as His6-tagged-maltose-binding fusion protein containing a tobacco etch virus protease cleavage site in Escherichia coli strain BL21(DE3) for the wild-type, and in strain B834(DE3) for a selenomethionine-labeled variant |
Thermoplasma acidophilum |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
6.3.4.21 |
recombinant detagged wild-type and selenomethionine-labeled enzymes, hanging drop vapour diffusion method, room temperature, 0.001 ml protein solution mixed with equal volume of well solution containing 0.2 M ammonium acetate, 0.1 M Tris-HCl, pH 8.5, 45% 2-methyl-2,4-pentanediol, 1 week, crystallization condition screening using sitting drop vapour diffusion at room temperature, iridium complexed, X-ray diffraction structure determination and analysis at 2.8 A resolution |
Thermoplasma acidophilum |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
6.3.4.21 |
43000 |
- |
6 * 43000, (alphabeta)3, SDS-PAGE and crystal structure analysis |
Thermoplasma acidophilum |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
6.3.4.21 |
Thermoplasma acidophilum |
Q9HJ28 |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
6.3.4.21 |
recombinant tagged wild-type and selenomethionine-labeled fusion enzymes from Escherichia coli to homogeneity by high-trap chelating nickel affinity chromatography |
Thermoplasma acidophilum |
Reaction
EC Number |
Reaction |
Comment |
Organism |
Reaction ID |
---|
6.3.4.21 |
nicotinate + 5-phospho-alpha-D-ribose 1-diphosphate + ATP + H2O = beta-nicotinate D-ribonucleotide + diphosphate + ADP + phosphate |
active site structure |
Thermoplasma acidophilum |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
6.3.4.21 |
hexamer |
6 * 43000, (alphabeta)3, SDS-PAGE and crystal structure analysis |
Thermoplasma acidophilum |
6.3.4.21 |
More |
enzyme monomer consists of 3 dimeric domains: an N-terminal alpha + beta domain, a central alpha/beta domain, and a C-terminal domain containing an unique zinc finger |
Thermoplasma acidophilum |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
6.3.4.21 |
NAPRTase |
- |
Thermoplasma acidophilum |