Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Gorrell, A.; Lawrence, S.H.; Ferry, J.G.
    Structural and kinetic analyses of arginine residues in the active site of the acetate kinase from Methanosarcina thermophila (2005), J. Biol. Chem., 280, 10731-10742.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.1 in presence of substrate and transition states analogues Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.1 R241A severe decrease in kinetic parameters Methanosarcina thermophila
2.7.2.1 R241L severe decrease in kinetic parameters Methanosarcina thermophila
2.7.2.1 R91A severe decrease in kinetic parameters Methanosarcina thermophila
2.7.2.1 R91K severe decrease in kinetic parameters Methanosarcina thermophila
2.7.2.1 R91L severe decrease in kinetic parameters Methanosarcina thermophila

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.1 adenosine 5'-(gamma-thio)triphosphate
-
 Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.016
-
 ATP mutant R91A, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.063
-
 ADP mutant R91A, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.063
-
 ADP mutant R91L, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.068
-
 ATP mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
2.7.2.1 0.068
-
 ATP wild-type, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
2.7.2.1 0.076
-
 ATP mutant R91K, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.08
-
 ATP wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.098
-
 ADP wild-type, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.145
-
 ATP mutant R91L, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.168
-
 ADP mutant R91K, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.21
-
 ADP mutant R241A, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.264
-
 ATP mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
2.7.2.1 0.297
-
 ATP mutant R241A, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.47
-
 acetyl phosphate wild-type, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.578
-
 ADP mutant R241K, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.61
-
 acetyl phosphate mutant R91K, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.84
-
 acetyl phosphate mutant R241A, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.92
-
 acetyl phosphate mutant R241K, pH 7.4 Methanosarcina thermophila
2.7.2.1 1.26
-
 ADP mutant R241L, pH 7.4 Methanosarcina thermophila
2.7.2.1 1.36
-
 acetyl phosphate mutant R91A, pH 7.4 Methanosarcina thermophila
2.7.2.1 1.64
-
 acetyl phosphate mutant R241L, pH 7.4 Methanosarcina thermophila
2.7.2.1 2.3
-
 acetyl phosphate mutant R91L, pH 7.4 Methanosarcina thermophila
2.7.2.1 2.7
-
 acetate wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 11.4
-
 ATP mutant R241K, pH 7.0 Methanosarcina thermophila
2.7.2.1 17
-
 ATP mutant R241L, pH 7.0 Methanosarcina thermophila
2.7.2.1 24
-
 acetate wild-type, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
2.7.2.1 30
-
 acetate mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
2.7.2.1 70
-
 acetate mutant R91K, pH 7.0 Methanosarcina thermophila
2.7.2.1 77
-
 acetate mutant R241K, pH 7.0 Methanosarcina thermophila
2.7.2.1 83
-
 acetate mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl Methanosarcina thermophila
2.7.2.1 250
-
 acetate mutant R91A, pH 7.0 Methanosarcina thermophila
2.7.2.1 270
-
 acetate mutant R241L, pH 7.0 Methanosarcina thermophila
2.7.2.1 420
-
 acetate mutant R91L, pH 7.0 Methanosarcina thermophila
2.7.2.1 710
-
 acetate mutant R241A, pH 7.0 Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Methanosarcina thermophila P38502
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 acetyl phosphate + hydroxylamine
-
 Methanosarcina thermophila acetyl hydroxamate + phosphate
-
 r
2.7.2.1 ATP + acetate
-
 Methanosarcina thermophila ADP + acetyl phosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.1 0.11
-
 ATP mutant R91A, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.22
-
 ATP mutant R91L, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.42
-
 ADP mutant R241L, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.68
-
 ATP mutant R241A, pH 7.0 Methanosarcina thermophila
2.7.2.1 1.3
-
 ATP mutant R241K, pH 7.0 Methanosarcina thermophila
2.7.2.1 1.38
-
 ATP mutant R241L, pH 7.0 Methanosarcina thermophila
2.7.2.1 3.5
-
 ADP mutant R91A, pH 7.4 Methanosarcina thermophila
2.7.2.1 3.7
-
 ATP mutant R91K, pH 7.0 Methanosarcina thermophila
2.7.2.1 4.5
-
 ADP mutant R241A, pH 7.4 Methanosarcina thermophila
2.7.2.1 4.5
-
 ADP mutant R241K, pH 7.4 Methanosarcina thermophila
2.7.2.1 7.7
-
 ADP mutant R91L, pH 7.4 Methanosarcina thermophila
2.7.2.1 23
-
 ADP mutant R91K, pH 7.4 Methanosarcina thermophila
2.7.2.1 913
-
 ATP wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 1260
-
 ADP wild-type, pH 7.4 Methanosarcina thermophila

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.2.1 0.24
-
 adenosine 5'-(gamma-thio)triphosphate pH 7.0 Methanosarcina thermophila