EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.14.12.18 | T335A | the ratio of the product formed from 2,2'-dichlorobiphenyl, 2,3-dihydroxy-2'-chlorobiphenyl to 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl is: 90/10 for the wild-type enzyme and 85/15 for the mutant enzymes | Paraburkholderia xenovorans |
1.14.12.18 | T335A/F336I | the ratio of the product formed from 2,2'-dichlorobiphenyl, 2,3-dihydroxy-2'-chlorobiphenyl to 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl is: 90/10 for the wild-type enzyme and 40/60 for the mutant enzymes | Paraburkholderia xenovorans |
1.14.12.18 | T335A/F336L | the ratio of the product formed from 2,2'-dichlorobiphenyl, 2,3-dihydroxy-2'-chlorobiphenyl to 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl is: 90/10 for the wild-type enzyme and 85/15 for the mutant enzymes | Paraburkholderia xenovorans |
1.14.12.18 | T335A/F336L/I341V | the ratio of the product formed from 2,2'-dichlorobiphenyl, 2,3-dihydroxy-2'-chlorobiphenyl to 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl is: 90/10 for the wild-type enzyme and 40/60 for the mutant enzymes | Paraburkholderia xenovorans |
1.14.12.18 | T335A/F336M | the ratio of the product formed from 2,2'-dichlorobiphenyl, 2,3-dihydroxy-2'-chlorobiphenyl to 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl is: 90/10 for the wild-type enzyme and 40/60 for the mutant enzymes | Paraburkholderia xenovorans |
1.14.12.18 | T335G | the ratio of the product formed from 2,2'-dichlorobiphenyl, 2,3-dihydroxy-2'-chlorobiphenyl to 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl is: 90/10 for the wild-type enzyme and 80/20 for the mutant enzymes | Paraburkholderia xenovorans |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.14.12.18 | Paraburkholderia xenovorans | - |
LB400 | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.14.12.18 | 2,2'-dichlorobiphenyl + NADH + H+ + O2 | - |
Paraburkholderia xenovorans | 2,3-dihydroxy-2'-chlorobiphenyl + 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl + NAD+ + HCl | the ratio of 2,3-dihydroxy-2'-chlorobiphenyl to 3,4-dihydro-3,4-dihydroxy-2,2'-dichlorobiphenyl is: 90/10 for the wild-type enzyme, 40/60 for the mutant enzymes T335A/F336M, T335A/F336L/I341V and T335A/F336I,80/20 for mutant enzyme T335G, 85/15 for the mutant enzymes T335A and T335A/F336L | ? | |
1.14.12.18 | 2,3,2',3'-tetrachlorobiphenyl + NADH + H+ + O2 | oxygenated onto meta-para carbons 4 and 5 | Paraburkholderia xenovorans | 4,5-dihydro-4,5-dihydroxy-2,3,2',3'-tetrachlorobiphenyl + NAD+ | - |
? | |
1.14.12.18 | 2,4,2',4'-tetrachlorobiphenyl + NADH + H+ + O2 | oxygenated principally onto vicinal ortho-meta carbons 2 and 3 | Paraburkholderia xenovorans | 2,3-dihydroxy-2',4,4'-trichlorobiphenyl + NAD+ + HCl | - |
? |