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Literature summary extracted from

  • Götting, C.; Müller, S.; Schöttler, M.; Schön, S.; Prante, C.; Brinkmann, T.; Kuhn, J.; Kleesiek, K.
    Analysis of the DXD motifs in human xylosyltransferase I required for enzyme activity (2004), J. Biol. Chem., 279, 42566-42573.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.26 D314G 80% of the activity of wild-type enzyme,Km-value for bikunin is 1.2fold higher than wild-type value Homo sapiens
2.4.2.26 D316G as active as wild-type enzyme, Km-value for bikunin is identical to wild-type value Homo sapiens
2.4.2.26 D745E mutant retains full activity, Km-value for bikunin is 1.2fold higher than wild-type value Homo sapiens
2.4.2.26 D745G loss of activity Homo sapiens
2.4.2.26 D747E reduced substrate affinity, about 35% of the wild-type activity, Km-value for bikunin is 4.4fold higher than wild-type value Homo sapiens
2.4.2.26 D747G reduced substrate affinity, about 35% of the wild-type activity, Km-value for bikunin is 7.7fold higher than wild-type value Homo sapiens
2.4.2.26 W746D about 25% of wild-type activity, Km-value for bikunin is 1.3fold higher than wild-type value Homo sapiens
2.4.2.26 W746G about 25% of wild-type activity, Km-value for bikunin is 1.3fold higher than wild-type value Homo sapiens
2.4.2.26 W746N about 25% of wild-type activity, Km-value for bikunin is 1.4fold higher than wild-type value Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.26 heparin
-
 Homo sapiens
2.4.2.26 UDP
-
 Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.26 0.0009
-
 bikunin pH 6.5, 37°C, wild-type enzyme Homo sapiens
2.4.2.26 0.0009
-
 bikunin pH 6.5, 37°C, mutant enzyme D316G Homo sapiens
2.4.2.26 0.0011
-
 bikunin pH 6.5, 37°C, mutant enzyme D314G Homo sapiens
2.4.2.26 0.0011
-
 bikunin pH 6.5, 37°C, mutant enzyme D745E Homo sapiens
2.4.2.26 0.0012
-
 bikunin pH 6.5, 37°C, mutant enzyme W746D Homo sapiens
2.4.2.26 0.0012
-
 bikunin pH 6.5, 37°C, mutant enzyme W746G Homo sapiens
2.4.2.26 0.0013
-
 bikunin pH 6.5, 37°C, mutant enzyme W746N Homo sapiens
2.4.2.26 0.0044
-
 bikunin pH 6.5, 37°C, mutant enzyme D747E Homo sapiens
2.4.2.26 0.0069
-
 bikunin pH 6.5, 37°C, mutant enzyme D747G Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.26 Homo sapiens Q86Y38
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.26 UDP-D-xylose + bikunin
-
 Homo sapiens UDP + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.26 XT-I
-
 Homo sapiens
2.4.2.26 xylosyltransferase I
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.26 0.00007
-
 heparin pH 6.5, 37°C, mutant enzyme D745E Homo sapiens
2.4.2.26 0.00011
-
 heparin pH 6.5, 37°C, mutant enzyme D747E Homo sapiens
2.4.2.26 0.00011
-
 heparin pH 6.5, 37°C, mutant enzyme W746N Homo sapiens
2.4.2.26 0.00012
-
 heparin pH 6.5, 37°C, mutant enzyme W746G Homo sapiens
2.4.2.26 0.00012
-
 heparin pH 6.5, 37°C, wild-type enzyme Homo sapiens
2.4.2.26 0.00013
-
 heparin pH 6.5, 37°C, mutant enzyme W746D Homo sapiens
2.4.2.26 0.00014
-
 heparin pH 6.5, 37°C, mutant enzyme D316G Homo sapiens
2.4.2.26 0.00015
-
 heparin pH 6.5, 37°C, mutant enzyme D314G Homo sapiens
2.4.2.26 0.00015
-
 heparin pH 6.5, 37°C, mutant enzyme D747G Homo sapiens
2.4.2.26 0.0019
-
 UDP pH 6.5, 37°C, mutant enzyme D745E Homo sapiens
2.4.2.26 0.0072
-
 UDP pH 6.5, 37°C, mutant enzyme D314G Homo sapiens
2.4.2.26 0.0108
-
 UDP pH 6.5, 37°C, wild-type enzyme Homo sapiens
2.4.2.26 0.0113
-
 UDP pH 6.5, 37°C, mutant enzyme D316G Homo sapiens
2.4.2.26 0.0147
-
 UDP pH 6.5, 37°C, mutant enzyme D747E Homo sapiens
2.4.2.26 0.0172
-
 UDP pH 6.5, 37°C, mutant enzyme D747G Homo sapiens
2.4.2.26 0.0179
-
 UDP pH 6.5, 37°C, mutant enzyme W746N Homo sapiens
2.4.2.26 0.0196
-
 UDP pH 6.5, 37°C, mutant enzyme W746D Homo sapiens
2.4.2.26 0.0246
-
 UDP pH 6.5, 37°C, mutant enzyme W746G Homo sapiens