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Literature summary extracted from

  • Fraaije, M.W.; Kamerbeek, N.M.; Heidekamp, A.J.; Fortin, R.; Janssen, D.B.
    The prodrug activator EtaA from Mycobacterium tuberculosis is a Baeyer-Villiger monooxygenase (2004), J. Biol. Chem., 279, 3354-3360.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.13.92 bovine serum albumin 2 mg7ml, 930% activation Mycobacterium tuberculosis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.92 expression in Escherichia coli Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.92 3-acetyl-NADP+ 0.5 mM, 50% inhibition Mycobacterium tuberculosis
1.14.13.92 3-amino-NADP+ 0.05 mM, 74% inhibition Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.92 0.061
-
 phenylacetone 25°C, pH 8.5 Mycobacterium tuberculosis
1.14.13.92 0.2
-
 2-dodecanone 25°C, pH 8.5 Mycobacterium tuberculosis
1.14.13.92 0.28
-
 phenylacetone 25°C, pH 8.5, in the presence of 0.006 mM bovine serum albumin Mycobacterium tuberculosis
1.14.13.92 0.34
-
 Ethionamide 25°C, pH 8.5 Mycobacterium tuberculosis
1.14.13.92 0.52
-
 benzylacetone 25°C, pH 8.5 Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.92 membrane associated Mycobacterium tuberculosis 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.92 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.92 Q-Sepharose, hydroxyapatite, Superdex 200 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.92 2-decanone + NADPH + O2
-
 Mycobacterium tuberculosis methyl nonanoate + octyl acetate + NADP+ + H2O
-
 ?
1.14.13.92 2-dodecanone + NADPH + O2
-
 Mycobacterium tuberculosis nonyl acetate + methyl decanoate + NADP+ + H2O
-
 ?
1.14.13.92 2-heptanone + NADPH + O2
-
 Mycobacterium tuberculosis pentyl acetate + NADP+ + H2O
-
 ?
1.14.13.92 2-hexanone + NADPH + O2
-
 Mycobacterium tuberculosis butyl acetate + NADP+ + H2O
-
 ?
1.14.13.92 2-octanone + NADPH + O2
-
 Mycobacterium tuberculosis heptyl acetate + NADP+ + H2O
-
 ?
1.14.13.92 3-octanone + NADPH + O2
-
 Mycobacterium tuberculosis ethyl hexanoate + pentyl propanoate + NADP+ + H2O
-
 ?
1.14.13.92 4-heptanone + NADPH + O2
-
 Mycobacterium tuberculosis propanyl butanoate + NADP+ + H2O
-
 ?
1.14.13.92 benzylacetone + NADPH + O2
-
 Mycobacterium tuberculosis ? + NADP+ + H2O
-
 ?
1.14.13.92 bicyclohept-2-en-6-one + NADPH + O2
-
 Mycobacterium tuberculosis 3-oxabicyclo[3.3.0]oct-6-en-2-one + NADP+ + H2O
-
 ?
1.14.13.92 ethionamide + NADPH + O2
-
 Mycobacterium tuberculosis ? + NADP+ + H2O
-
 ?
1.14.13.92 methyl-p-tolylsulfide + NADPH + O2
-
 Mycobacterium tuberculosis ? + NADP+ + H2O
-
 ?
1.14.13.92 phenylacetone + NADPH + H+ + O2
-
 Mycobacterium tuberculosis benzyl acetate + NADP+ + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.13.92 EtaA
-
 Mycobacterium tuberculosis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.14.13.92 0.017
-
 phenylacetone 25°C, pH 8.5 Mycobacterium tuberculosis
1.14.13.92 0.021
-
 benzylacetone 25°C, pH 8.5 Mycobacterium tuberculosis
1.14.13.92 0.023
-
 2-dodecanone 25°C, pH 8.5 Mycobacterium tuberculosis
1.14.13.92 0.027
-
 Ethionamide 25°C, pH 8.5 Mycobacterium tuberculosis
1.14.13.92 0.26
-
 phenylacetone 25°C, pH 8.5, in the presence of 0.006 mM bovine serum albumin Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.92 FAD
-
 Mycobacterium tuberculosis
1.14.13.92 NADPH
-
 Mycobacterium tuberculosis