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Literature summary extracted from

  • Sweet, C.R.; Williams, A.H.; Karbarz, M.J.; Werts, C.; Kalb, S.R.; Cotter, R.J.; Raetz, C.R.
    Enzymatic synthesis of lipid A molecules with four amide-linked acyl chains. LpxA acyltransferases selective for an analog of UDP-N-acetylglucosamine in which an amine replaces the 3"-hydroxyl group (2004), J. Biol. Chem., 279, 25411-25419.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.129 gene lpxA, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) Acidithiobacillus ferrooxidans
2.3.1.129 gene lpxA, expression in Escherichia coli strain BL21(DE3) Leptospira interrogans
2.3.1.129 gene lpxA, expression in strain BL21(DE3) Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.129 additional information heterologous expression of Acidithiobacillus ferrooxidans' genes lpxA, gnnA, and gnnB results in occurence of UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose utilizing activity in Escherichia coli cells with defective lpxA gene in vivo Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.129 (R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Leptospira interrogans first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Mesorhizobium loti first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Acidithiobacillus ferrooxidans first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine Escherichia coli
-
 [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.129 Acidithiobacillus ferrooxidans Q6QHI7
-
-
2.3.1.129 Escherichia coli
-
-
-
2.3.1.129 Leptospira interrogans
-
-
-
2.3.1.129 Mesorhizobium loti
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.129 a (3R)-3-hydroxyacyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier protein] + a UDP-3-O-[(3R)-3-hydroxyacyl]-N-acetyl-alpha-D-glucosamine mechanism involving essential His125 Leptospira interrogans
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.129 additional information
-
 activity of the recombinant enzyme Acidithiobacillus ferrooxidans
2.3.1.129 additional information
-
 substrate specificity with different acyl acceptors Mesorhizobium loti
2.3.1.129 additional information
-
 substrate specificity with different acyl acceptors, activity of the recombinant enzyme Escherichia coli
2.3.1.129 additional information
-
 substrate specificity with different acyl acceptors, activity of the recombinant enzyme Leptospira interrogans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.129 (R)-3-hydroxydecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Mesorhizobium loti [acyl-carrier protein] + UDP-3-N-(3-hydroxydecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Mesorhizobium loti [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units Leptospira interrogans [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose the enzyme is absolute specific for 3-hydroxylauroyl-ACP as donor substrate Leptospira interrogans [acyl-carrier protein] + UDP-3-N-(3-hydroxydodecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxydodecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxydodecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
2.3.1.129 (R)-3-hydroxyhexadecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxyhexadecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 Mesorhizobium loti [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units Mesorhizobium loti [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose first step in lipid A biosynthesis pathway, lipid A is an unusual variant in this species containing 2,3-diamino-2,3-dideoxy-D-glucopyranose units Acidithiobacillus ferrooxidans [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose only in vitro Escherichia coli [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose specific for UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose Acidithiobacillus ferrooxidans [acyl-carrier protein] + UDP-3-N-(3-hydroxytetradecanoyl)-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine
-
 Escherichia coli [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
2.3.1.129 (R)-3-hydroxytetradecanoyl-[acyl-carrier protein] + UDP-N-acetyl-alpha-D-glucosamine low activity Acidithiobacillus ferrooxidans [acyl-carrier protein] + UDP-3-O-(3-hydroxytetradecanoyl)-N-acetyl-alpha-D-glucosamine
-
 ?
2.3.1.129 additional information substrate specificity, lipid A structure overview Acidithiobacillus ferrooxidans ?
-
 ?
2.3.1.129 additional information substrate specificity, no activity with UDP-2-acetamido-3-amino-2,3-dideoxy-alpha-D-glucopyranose due to lack of genes gnnA and gnnB, lipid A structure overview Escherichia coli ?
-
 ?
2.3.1.129 additional information substrate specificity, no activity with UDP-N-acetylglucosamine, equally effective with 3-hydroxyacyl-ACP of different chain lengths, lipid A structure overview Mesorhizobium loti ?
-
 ?
2.3.1.129 additional information substrate specificity, no activity with UDP-N-acetylglucosamine, lipid A structure overview Leptospira interrogans ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.129 LpxA
-
 Escherichia coli
2.3.1.129 LpxA
-
 Mesorhizobium loti
2.3.1.129 LpxA
-
 Leptospira interrogans
2.3.1.129 LpxA
-
 Acidithiobacillus ferrooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.129 30
-
 assay at Escherichia coli
2.3.1.129 30
-
 assay at Mesorhizobium loti
2.3.1.129 30
-
 assay at Leptospira interrogans
2.3.1.129 30
-
 assay at Acidithiobacillus ferrooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.129 8
-
 assay at Escherichia coli
2.3.1.129 8
-
 assay at Mesorhizobium loti
2.3.1.129 8
-
 assay at Leptospira interrogans
2.3.1.129 8
-
 assay at Acidithiobacillus ferrooxidans