EC Number | Cloned (Comment) | Organism |
---|---|---|
1.18.6.1 | overexpression of Azotobacter vinelandii NafY protein in Escherichia coli strain BL21(DE3) | Azotobacter vinelandii |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.18.6.1 | Iron | enzyme contains an iron-molybdenum cofactor | Azotobacter vinelandii | |
1.18.6.1 | Molybdenum | enzyme contains an iron-molybdenum cofactor | Azotobacter vinelandii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.18.6.1 | 44000 | - |
FeMo cofactor-NafY protein complex, gel filtration | Azotobacter vinelandii |
1.18.6.1 | 46000 | - |
NafY protein, gel filtration, sedimentation equilibrium centrifugation | Azotobacter vinelandii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.18.6.1 | Azotobacter vinelandii | - |
- |
- |
1.18.6.1 | Azotobacter vinelandii DJ | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.18.6.1 | recombinant Azotobacter vinelandii NafY protein from Escherichia coli strain BL21(DE3) | Azotobacter vinelandii |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.18.6.1 | dimer | 2 * 26141-28000, unassociated NafY protein, sequence calculation and sedimentation equilibrium centrifugation | Azotobacter vinelandii |
1.18.6.1 | More | the NafY protein monomerizes upon binding to the FeMo cofactor | Azotobacter vinelandii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.18.6.1 | More | the nitrogenase complex is composed of 2 oxygen-labile metalloproteins: dinitrogenase and dinitrogenase reductase | Azotobacter vinelandii |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.18.6.1 | iron-molybdenum cofactor | required for activity of the enzyme complex, insertion in the presynthesized apodinitrogenase involving the monomeric 26 kDa NafY protein, which binds the FeMo cofactor with very high affinity via its HIs121, the cofactor-NafY-complex exhibits an EPR signal similar to isolated FeMo cofactor and the M-center of the enzyme, NafY also binds the biosynthetic precursor of the MoFe cofactor, the NifB-cofacor, determination of binding sites | Azotobacter vinelandii |