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Literature summary extracted from

  • Kalscheuer, R.; Steinbuchel, A.
    A novel bifunctional wax ester synthase/acyl-CoA:diacylglycerol acyltransferase mediates wax ester and triacylglycerol biosynthesis in Acinetobacter calcoaceticus ADP1 (2003), J. Biol. Chem., 278, 8075-8082.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.20
-
 Acinetobacter baylyi ADP1
2.3.1.75
-
 Acinetobacter baylyi ADP1
2.3.1.332 expressed in Pseudomonas citronellolis Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.20 0.0211
-
 palmitoyl-CoA pH 7.4, 35°C Acinetobacter baylyi ADP1
2.3.1.75 0.0156
-
 palmitoyl-CoA pH 7.4, 35°C Acinetobacter baylyi ADP1
2.3.1.332 0.0156
-
 palmitoyl-CoA with 1-hexadecanol as cosubstrate, pH and temperature not specified in the publication Acinetobacter calcoaceticus
2.3.1.332 0.0211
-
 palmitoyl-CoA with 1,2-dipalmitoyl-rac-glycerol as cosubstrate, pH and temperature not specified in the publication Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.20 51800
-
 x * 51800, deduced from gene sequence Acinetobacter baylyi ADP1
2.3.1.75 51800
-
 x * 51800, deduced from gene sequence Acinetobacter baylyi ADP1

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Acinetobacter baylyi ADP1 Q8GGG1 bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC2.3.1.20 resp
-
2.3.1.75 Acinetobacter baylyi ADP1 Q8GGG1 bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC 2.3.1.20 resp.
-
2.3.1.332 Acinetobacter calcoaceticus
-
-
-
2.3.1.332 Acinetobacter calcoaceticus ADP1
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.20 0.0543
-
 pH 7.4, 35°C Acinetobacter baylyi ADP1
2.3.1.75 0.2128
-
 pH 7.4, 35°C Acinetobacter baylyi ADP1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 1,2-dipalmitoyl-rac-glycerol + palmitoyl-CoA
-
 Acinetobacter baylyi ADP1 tripalmitoylglycerol + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Acinetobacter baylyi ADP1 hexadecyl palmitate + CoA
-
 ?
2.3.1.332 arachidoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus CoA + hexadecyl arachidoate
-
 ?
2.3.1.332 arachidoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus ADP1 CoA + hexadecyl arachidoate
-
 ?
2.3.1.332 lauroyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus CoA + hexadecyl laurate
-
 ?
2.3.1.332 lauroyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus ADP1 CoA + hexadecyl laurate
-
 ?
2.3.1.332 additional information the wax synthase accepts a wide range of various chain length acyl-CoA molecules (C12-C:20) almost equally Acinetobacter calcoaceticus ?
-
-
2.3.1.332 myristoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus CoA + hexadecyl tetradecanoate
-
 ?
2.3.1.332 myristoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus ADP1 CoA + hexadecyl tetradecanoate
-
 ?
2.3.1.332 oleoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus CoA + hexadecyl oleate
-
 ?
2.3.1.332 palmitoleoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus CoA + hexadecyl palmitoleoate
-
 ?
2.3.1.332 palmitoyl-CoA + 1,2-dipalmitoyl-rac-glycerol
-
 Acinetobacter calcoaceticus CoA + 1,2,3-tripalmitoyl-rac-glycerol
-
 ?
2.3.1.332 palmitoyl-CoA + 1-dodecanol
-
 Acinetobacter calcoaceticus CoA + dodecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus CoA + hexadecyl hexdecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus ADP1 CoA + hexadecyl hexdecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + 1-hexadecenol
-
 Acinetobacter calcoaceticus CoA + hexadecenyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + 1-octadecenol
-
 Acinetobacter calcoaceticus CoA + octadecenyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + 1-octdecanol
-
 Acinetobacter calcoaceticus CoA + octadecyl hexadecanoate
-
 ?
2.3.1.332 palmitoyl-CoA + 1-tetradecanol
-
 Acinetobacter calcoaceticus CoA + tetradecyl hexadecanoate
-
 ?
2.3.1.332 stearoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus CoA + hexadecyl octadecanoate
-
 ?
2.3.1.332 stearoyl-CoA + 1-hexadecanol
-
 Acinetobacter calcoaceticus ADP1 CoA + hexadecyl octadecanoate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 ? x * 51800, deduced from gene sequence Acinetobacter baylyi ADP1
2.3.1.75 ? x * 51800, deduced from gene sequence Acinetobacter baylyi ADP1
2.3.1.332 ? x * 51800, calculated from amino acid sequence Acinetobacter calcoaceticus

Synonyms

EC Number Synonyms Comment Organism
2.3.1.332 wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
 Acinetobacter calcoaceticus
2.3.1.332 wax/dgat
-
 Acinetobacter calcoaceticus
2.3.1.332 WS/DGAT the bifunctional enzyme exhibits acyl-CoA:fatty alcohol acyltransferase (wax ester synthase) as well as acyl-CoA:diacylglycerol acyltransferase activity Acinetobacter calcoaceticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.3.1.20 Acinetobacter baylyi ADP1 calculated
-
 9.05
2.3.1.75 Acinetobacter baylyi ADP1 calculated
-
 9.05
2.3.1.332 Acinetobacter calcoaceticus calculated from amino acid sequence
-
 9.1