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Literature summary extracted from

  • Chaturvedi, S.; Bhakuni, V.
    Unusual structural, functional, and stability properties of serine hydroxymethyltransferase from Mycobacterium tuberculosis (2003), J. Biol. Chem., 278, 40793-40805.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.2.1 SHM1 Mycobacterium tuberculosis
2.1.2.1 SHM2 Mycobacterium tuberculosis

General Stability

EC Number General Stability Organism
2.1.2.1 guanidinium chloride-induced two-step unfolding of SHM1 with the first step being dissociation of dimer into apomonomer at low denaturant concentrations followed by unfolding of the stabilized monomer at higher denaturant concentrations, SHM1 Mycobacterium tuberculosis
2.1.2.1 guanidinium chloride-induced two-step unfolding of SHM1 with the first step being dissociation of dimer into apomonomer at low denaturant concentrations followed by unfolding of the stabilized monomer at higher denaturant concentrations, SHM2 Mycobacterium tuberculosis
2.1.2.1 urea-induced two-step unfolding of SHM1 with the first step being dissociation of dimer into apomonomer at low denaturant concentrations followed by unfolding of the stabilized monomer at higher denaturant concentrations. The enzyme-bound pyridoxal 5'-phosphate gets dissociated from the enzyme on treatment with about 1.25 M urea, SHM1 Mycobacterium tuberculosis
2.1.2.1 urea-induced two-step unfolding of SHM1 with the first step being dissociation of dimer into apomonomer at low denaturant concentrations followed by unfolding of the stabilized monomer at higher denaturant concentrations. The enzyme-bound pyridoxal 5'-phosphate gets dissociated from the enzyme on treatment with about 1.25 M urea, SHM2 Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.2.1 45000
-
 2 * 45000, SHM1, SDS-PAGE Mycobacterium tuberculosis
2.1.2.1 45500
-
 2 * 45500, SHM2, SDS-PAGE Mycobacterium tuberculosis
2.1.2.1 87100
-
 SHM2, gel filtration Mycobacterium tuberculosis
2.1.2.1 91200
-
 SHM1, gel filtration Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.1.2.1 Mycobacterium tuberculosis P9WGI7
-
-
2.1.2.1 Mycobacterium tuberculosis P9WGI9
-
-
2.1.2.1 Mycobacterium tuberculosis H37Rv P9WGI7
-
-
2.1.2.1 Mycobacterium tuberculosis H37Rv P9WGI9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.2.1 SHM1 Mycobacterium tuberculosis
2.1.2.1 SHM2 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.2.1 additional information SHM1 does not undergo half-transamination reaction with D-Ala resulting in the formation of the apoenzyme Mycobacterium tuberculosis ?
-
 ?
2.1.2.1 additional information SHM2 does not undergo half-transamination reaction with D-Ala resulting in the formation of the apoenzyme Mycobacterium tuberculosis ?
-
 ?
2.1.2.1 additional information SHM2 does not undergo half-transamination reaction with D-Ala resulting in the formation of the apoenzyme Mycobacterium tuberculosis H37Rv ?
-
 ?
2.1.2.1 additional information SHM1 does not undergo half-transamination reaction with D-Ala resulting in the formation of the apoenzyme Mycobacterium tuberculosis H37Rv ?
-
 ?
2.1.2.1 tetrahydrofolate + L-Ser
-
 Mycobacterium tuberculosis 5,10-methylenetetrahydrofolate + glycine
-
 r
2.1.2.1 tetrahydrofolate + L-Ser
-
 Mycobacterium tuberculosis H37Rv 5,10-methylenetetrahydrofolate + glycine
-
 r

Subunits

EC Number Subunits Comment Organism
2.1.2.1 dimer 2 * 45000, SHM1, SDS-PAGE Mycobacterium tuberculosis
2.1.2.1 dimer 2 * 45500, SHM2, SDS-PAGE Mycobacterium tuberculosis

Synonyms

EC Number Synonyms Comment Organism
2.1.2.1 glycine hydroxymethyltransferrase
-
 Mycobacterium tuberculosis
2.1.2.1 serine hydroxymethyltransferase
-
 Mycobacterium tuberculosis
2.1.2.1 SHM1
-
 Mycobacterium tuberculosis
2.1.2.1 SHM2
-
 Mycobacterium tuberculosis
2.1.2.1 SHMT1
-
 Mycobacterium tuberculosis
2.1.2.1 SHMT2
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.1.2.1 60
-
 transition temperature of SHM1 Mycobacterium tuberculosis
2.1.2.1 69
-
 transition temperature of SHM1 Mycobacterium tuberculosis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
2.1.2.1 8
-
 transition of SHM2 is between pH 6.5 and 11 and centered at about pH 8.0 Mycobacterium tuberculosis
2.1.2.1 9
-
 native SHM2 dimer dissociates into monomer Mycobacterium tuberculosis
2.1.2.1 9.25
-
 transition of SHM1 is between pH 8 and 11 and centered at about pH 9.25 Mycobacterium tuberculosis
2.1.2.1 10.5
-
 resistant to alkaline denaturation up to, SHM1 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.1.2.1 pyridoxal 5'-phosphate SHM1 contains 1 mol per mol of enzyme dimer Mycobacterium tuberculosis
2.1.2.1 pyridoxal 5'-phosphate SHM2 contains 2 mol per mol of enzyme dimer Mycobacterium tuberculosis