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Literature summary extracted from

  • O'Byrne, J.; Hunt, M.C.; Rai, D.K.; Saeki, M.; Alexson, S.E.H.
    The Human bile acid-CoA:amino acid N-acyltransferase functions in the conjugation of fatty acids to glycine (2003), J. Biol. Chem., 278, 34237-34244.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.65 expression of wild-type and mutant enzymes Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.65 C235A activity is less than 0.4% of the wild-type activity Homo sapiens
2.3.1.65 C235S mutation converts the enzyme into an efficient bile acid-CoA thioesterase Homo sapiens
2.3.1.65 D326A activity is less than 0.4% of the wild-type activity Homo sapiens
2.3.1.65 H362Q activity is less than 0.4% of the wild-type activity Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.65 cytosol
-
 Mus musculus 5829
-
2.3.1.65 cytosol
-
 Homo sapiens 5829
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.65 additional information Mus musculus may play important roles in protection against toxicity by accumulation of unconjugated bile acids and non-esterified very long-chain fatty acids ?
-
 ?
2.3.1.65 additional information Homo sapiens may play important roles in protection against toxicity by accumulation of unconjugated bile acids and non-esterified very long-chain fatty acids ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.65 Homo sapiens
-
 recombinant enzyme
-
2.3.1.65 Mus musculus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.65
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.65 gall bladder
-
 Mus musculus
-
2.3.1.65 gall bladder epithelium
-
 Homo sapiens
-
2.3.1.65 intestine
-
 Mus musculus
-
2.3.1.65 kidney
-
 Mus musculus
-
2.3.1.65 liver
-
 Mus musculus
-
2.3.1.65 liver
-
 Homo sapiens
-
2.3.1.65 pancreas
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.65 1.041
-
 activity with choloyl-CoA anf glycine as substrates Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.65 chenodeoxycholoyl-CoA + glycine glycine is a slightly better substrate than taurine, thioesterase activity in absence of glycine is about 20% of the conjugation activity Homo sapiens CoA + glycochenodeoxycholate
-
 ?
2.3.1.65 chenodeoxycholoyl-CoA + taurine
-
 Homo sapiens CoA + taurochenodeoxycholate
-
 ?
2.3.1.65 choloyl-CoA + glycine glycine is a slightly better substrate than taurine, thioesterase activity in absence of glycine is about 20% of the conjugation activity Homo sapiens CoA + glycocholate
-
 ?
2.3.1.65 choloyl-CoA + taurine
-
 Homo sapiens CoA + taurocholate
-
 ?
2.3.1.65 additional information may play important roles in protection against toxicity by accumulation of unconjugated bile acids and non-esterified very long-chain fatty acids Mus musculus ?
-
 ?
2.3.1.65 additional information may play important roles in protection against toxicity by accumulation of unconjugated bile acids and non-esterified very long-chain fatty acids Homo sapiens ?
-
 ?
2.3.1.65 additional information in addition to its bile acid conjugating activity, the enzyme also possesses thioesterase and glycine conjugating activities with long- and very long-chain acyl-CoAs, at least in vitro. The enzyme can also hydrolyze long- and very-long-chain saturated acyl-CoAs (mainly C16:0-C26:0), the enzyme also conjugates fatty acids to glycine Homo sapiens ?
-
 ?
2.3.1.65 trihydroxycholestanoyl-CoA + glycine very low activity Homo sapiens CoA + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.65 BACAT
-
 Mus musculus
2.3.1.65 BACAT
-
 Homo sapiens