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Literature summary extracted from

  • Hatta, T.; Mukerjee-Dhar, G.; Damborsky, J.; Kiyohara, H.; Kimbara, K.
    Characterization of a novel thermostable Mn(II)-dependent 2,3-dihydroxybiphenyl 1,2-dioxygenase from a polychlorinated biphenyl- and naphthalene-degrading Bacillus sp. JF8 (2003), J. Biol. Chem., 278, 21483-21492.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.39 expression in Escherichia coli Bacillus sp. (in: Bacteria)

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.39 0.000095
-
 2,3-Dihydroxybiphenyl 60°C, pH 7.5 Bacillus sp. (in: Bacteria)
1.13.11.39 0.001
-
 3-methylcatechol 60°C, pH 7.5 Bacillus sp. (in: Bacteria)
1.13.11.39 0.025
-
 4-Chlorocatechol 60°C, pH 7.5 Bacillus sp. (in: Bacteria)
1.13.11.39 0.1
-
 catechol 60°C, pH 7.5 Bacillus sp. (in: Bacteria)
1.13.11.39 0.11
-
 4-methylcatechol 60°C, pH 7.5 Bacillus sp. (in: Bacteria)

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.13.11.39 Manganese enzyme contains 4.0-4.8 manganese atoms per enzyme molecule, depending on the batch Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.39 35000
-
 4 * 35000, SDS-PAGE Bacillus sp. (in: Bacteria)
1.13.11.39 125000
-
 gel filtration Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.39 Bacillus sp. (in: Bacteria) Q8GR45 JF8
-
1.13.11.39 Bacillus sp. (in: Bacteria) JF8 Q8GR45 JF8
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.39 DEAE-Toyopearl, Phenyl-Sepharose, Mono Q Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.39 5.47
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.39 2,3-dihydroxybiphenyl + O2
-
 Bacillus sp. (in: Bacteria) 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 2,3-dihydroxybiphenyl + O2
-
 Bacillus sp. (in: Bacteria) JF8 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate + H2O
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.13.11.39 3-methylcatechol + O2
-
 Bacillus sp. (in: Bacteria) JF8 ?
-
 ?
1.13.11.39 4-chlorocatechol + O2
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.13.11.39 4-chlorocatechol + O2
-
 Bacillus sp. (in: Bacteria) JF8 ?
-
 ?
1.13.11.39 4-methylcatechol + O2
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.13.11.39 4-methylcatechol + O2
-
 Bacillus sp. (in: Bacteria) JF8 ?
-
 ?
1.13.11.39 catechol + O2
-
 Bacillus sp. (in: Bacteria) ?
-
 ?
1.13.11.39 catechol + O2
-
 Bacillus sp. (in: Bacteria) JF8 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.39 tetramer 4 * 35000, SDS-PAGE Bacillus sp. (in: Bacteria)

Synonyms

EC Number Synonyms Comment Organism
1.13.11.39 BphC_JF8
-
 Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.39 85
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.39 60
-
 no loss of activity after 1 h Bacillus sp. (in: Bacteria)
1.13.11.39 70
-
 25% loss of activity after 1 h Bacillus sp. (in: Bacteria)
1.13.11.39 80
-
 approx. 60% loss of activity after 30 min Bacillus sp. (in: Bacteria)

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.39 7.5
-
-
 Bacillus sp. (in: Bacteria)