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Literature summary extracted from

  • Hibino, T.; Waditee, R.; Araki, E.; Ishikawa, H.; Aoki, K.; Tanaka, Y.; Takabe, T.
    Functional characterization of choline monooxygenase, an enzyme for betaine synthesis in plants (2002), J. Biol. Chem., 277, 41352-41360.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.15.7 expression in Escherichia coli, Synechococcus sp. PCC7942 and Arabidopsis thaliana Spinacia oleracea

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.7 C181A second Cys in the putative consensus sequence for coordination of Rieske-type cluster to Ser, Ala and Thr, essential for activity Spinacia oleracea
1.14.15.7 C181S second Cys in the putative consensus sequence for coordination of Rieske-type cluster to Ser, Ala and Thr, essential for activity Spinacia oleracea
1.14.15.7 C181T second Cys in the putative consensus sequence for coordination of Rieske-type cluster to Ser, Ala and Thr, essential for activity Spinacia oleracea
1.14.15.7 H287A first His in the putative consensus sequence for coordination of mononuclear non-heme Fe to Gly, Ala, and Val, His-287 is essential for activity Spinacia oleracea
1.14.15.7 H287G first His in the putative consensus sequence for coordination of mononuclear non-heme Fe to Gly, Ala, and Val, His-287 is essential for activity Spinacia oleracea
1.14.15.7 H287V first His in the putative consensus sequence for coordination of mononuclear non-heme Fe to Gly, Ala, and Val, His-287 is essential for activity Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.15.7 Iron enzyme contains a Rieske-type iron-sulfur center Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.15.7 choline + O2 + reduced ferredoxin Spinacia oleracea
-
betaine aldehyde hydrate + H2O + oxidized ferredoxin
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.7 Spinacia oleracea O04121
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.15.7 leaf
-
Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.7 choline + O2 + reduced ferredoxin
-
Spinacia oleracea betaine aldehyde hydrate + H2O + oxidized ferredoxin
-
?

Synonyms

EC Number Synonyms Comment Organism
1.14.15.7 CMO
-
Spinacia oleracea