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Literature summary extracted from

  • Kordulakova, J.; Gilleron, M.; Mikusova, K.; Puzo, G.; Brennan, P.J.; Gicquel, B.; Jackson, M.
    Definition of the first mannosylation step in phosphatidylinositol: Mannoside Synthesis. PimA IS essential for growth of mycobacteria (2002), J. Biol. Chem., 277, 31335-31344.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.1.345 analysis development of a cell-free assay. Membranes from Mycobacterium smegmatis overexpressing the pimA gene incorporate mannose from GDP-[14C]Man into di- and tri-acylated phosphatidylinositol mono-mannosides Mycolicibacterium smegmatis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.345 expression in Escherichia coli Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.345 membrane sequence contains a putative transmembrane segment, residues 275-298 Mycolicibacterium smegmatis 16020
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Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.345 40000
-
 x * 40000, SDS-PAGE Mycolicibacterium smegmatis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.345 GDP-mannose + phosphatidylinositol Mycolicibacterium smegmatis involved in the early steps of phosphatidylinositol mannoside synthesis, the synthesis of phosphatidylinositol monomannosides and derived higher phosphatidylinositol mannosides in M. smegmatis appears to be dependent on PimA and essential for growth GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.345 Mycolicibacterium smegmatis
-
-
-
2.4.1.345 Mycolicibacterium smegmatis A0QWG6
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-
2.4.1.345 Mycolicibacterium smegmatis ATCC 700084 A0QWG6
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-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.345 GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol
-
 Mycolicibacterium smegmatis GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
 ?
2.4.1.345 GDP-alpha-D-mannose + 1-phosphatidyl-1D-myo-inositol
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 Mycolicibacterium smegmatis ATCC 700084 GDP + 2-O-(alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
 ?
2.4.1.345 GDP-mannose + phosphatidylinositol
-
 Mycolicibacterium smegmatis GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
-
 ?
2.4.1.345 GDP-mannose + phosphatidylinositol involved in the early steps of phosphatidylinositol mannoside synthesis, the synthesis of phosphatidylinositol monomannosides and derived higher phosphatidylinositol mannosides in M. smegmatis appears to be dependent on PimA and essential for growth Mycolicibacterium smegmatis GDP + phosphatidyl-(2-O-alpha-D-manno-pyranosyl)-myo-inositol
-
 ?
2.4.1.345 additional information the PimA enzyme catalyzes the transfer of the first Man residue from GDP-Man to the myo-inositol residue of phosphatidylinositol, yielding diacylated forms of phosphatidylinositol mono-mannoside Mycolicibacterium smegmatis ?
-
 ?
2.4.1.345 additional information the PimA enzyme catalyzes the transfer of the first Man residue from GDP-Man to the myo-inositol residue of phosphatidylinositol, yielding diacylated forms of phosphatidylinositol mono-mannoside Mycolicibacterium smegmatis ATCC 700084 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.345 ? x * 40000, SDS-PAGE Mycolicibacterium smegmatis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.345 alpha-D-mannose-alpha(1,2)-phosphatidyl-myoinositol transferase
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 Mycolicibacterium smegmatis
2.4.1.345 PimA
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 Mycolicibacterium smegmatis
2.4.1.345 Rv2610c enzyme
-
 Mycolicibacterium smegmatis

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.4.1.345 Mycolicibacterium smegmatis calculated, N-terminal half of the protein, residues 1-202
-
 9.5

General Information

EC Number General Information Comment Organism
2.4.1.345 physiological function PimA is involved in the metabolic pathway producing phosphatidylinositol dimannoside PIM2 and phosphatidylinositol hexamannosidePIM6. Crude extracts from Escherichia coli producing recombinant PimA protein synthesize diacylated phosphatidylinositol mono-mannoside from GDP-[14C]Man and bovine phosphatidylinositol. A conditional mutant is unable to grow at the higher temperature at which the rescue plasmid is lost. The synthesis of phosphatidylinositol mono-mannosides and derived higher phosphatidylinositol mannosides appears to be dependent on PimA and essential for growth Mycolicibacterium smegmatis