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Literature summary extracted from

  • Sarin, J.; Aggarwal, S.; Chaba, R.; Varshney, G.C.; Chakraborti, P.K.
    B-subunit of phosphate-specific transporter from Mycobacterium tuberculosis is a thermostable ATPase (2001), J. Biol. Chem., 276, 44590-44597.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
7.3.2.1 overexpression in Escherichia coli Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
7.3.2.1 D188K no ATP hydrolyzing ability Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
7.3.2.1 5'-p-fluorosulfonylbenzoyl adenosine pH 7.4, 30 min at 30°C, 1-3 mM Mycobacterium tuberculosis
7.3.2.1 guanidine hydrochloride 5 min at 100°C, 8000 mM, significant reduction in the activity Mycobacterium tuberculosis
7.3.2.1 Mn2+ at room temperature Mycobacterium tuberculosis
7.3.2.1 additional information no inhibition with sodium azide, sodium orthovanadate and N-ethylmaleimide Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
7.3.2.1 0.0239
-
 ATP at 37 °C, pH 7.5 Escherichia coli
7.3.2.1 0.0715
-
 ATP at 37 °C, pH 7.5 Mycobacterium tuberculosis
7.3.2.1 0.13
-
 CTP at 37 °C, pH 7.5 Mycobacterium tuberculosis
7.3.2.1 0.211
-
 GTP at 37 °C, pH 7.5 Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
7.3.2.1 inclusion body
-
 Escherichia coli 16234
-
7.3.2.1 inclusion body
-
 Mycobacterium tuberculosis 16234
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
7.3.2.1 additional information no activation with Ca2+, Mg2+ Mycobacterium tuberculosis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
7.3.2.1 30500
-
 PstB is the ATP-binding component, 2 * 30500, SDS-PAGE Mycobacterium tuberculosis
7.3.2.1 69300
-
 gel filtration chromatography Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
7.3.2.1 ATP + H2O + phosphate/out Escherichia coli ATP-consuming import of phosphate ADP + phosphate + phosphate/in
-
 ?
7.3.2.1 ATP + H2O + phosphate/out Mycobacterium tuberculosis ATP-consuming import of phosphate ADP + phosphate + phosphate/in
-
 ?
7.3.2.1 ATP + H2O + phosphate/out Mycobacterium tuberculosis H37Rv ATP-consuming import of phosphate ADP + phosphate + phosphate/in
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
7.3.2.1 Escherichia coli
-
-
-
7.3.2.1 Mycobacterium tuberculosis
-
-
-
7.3.2.1 Mycobacterium tuberculosis H37Rv
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
7.3.2.1 no modification
-
 Escherichia coli
7.3.2.1 no modification
-
 Mycobacterium tuberculosis

Purification (Commentary)

EC Number Purification (Comment) Organism
7.3.2.1 by gel filtration chromatography using Superdex 200 column Escherichia coli
7.3.2.1 by gel permeation chromatography using Superdex 200 column Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
7.3.2.1 ATP + H2O + phosphate/out
-
 Escherichia coli ADP + phosphate + phosphate/in
-
 ?
7.3.2.1 ATP + H2O + phosphate/out
-
 Mycobacterium tuberculosis ADP + phosphate + phosphate/in
-
 ?
7.3.2.1 ATP + H2O + phosphate/out ATP-consuming import of phosphate Escherichia coli ADP + phosphate + phosphate/in
-
 ?
7.3.2.1 ATP + H2O + phosphate/out ATP-consuming import of phosphate Mycobacterium tuberculosis ADP + phosphate + phosphate/in
-
 ?
7.3.2.1 ATP + H2O + phosphate/out
-
 Mycobacterium tuberculosis H37Rv ADP + phosphate + phosphate/in
-
 ?
7.3.2.1 ATP + H2O + phosphate/out ATP-consuming import of phosphate Mycobacterium tuberculosis H37Rv ADP + phosphate + phosphate/in
-
 ?
7.3.2.1 CTP + H2O + phosphate/out
-
 Mycobacterium tuberculosis CDP + phosphate + phosphate/in
-
 ?
7.3.2.1 CTP + H2O + phosphate/out
-
 Mycobacterium tuberculosis H37Rv CDP + phosphate + phosphate/in
-
 ?
7.3.2.1 GTP + H2O + phosphate/out
-
 Mycobacterium tuberculosis GDP + phosphate + phosphate/in
-
 ?
7.3.2.1 GTP + H2O + phosphate/out
-
 Mycobacterium tuberculosis H37Rv GDP + phosphate + phosphate/in
-
 ?

Subunits

EC Number Subunits Comment Organism
7.3.2.1 dimer PstB is the ATP-binding component, 2 * 30500, SDS-PAGE Mycobacterium tuberculosis
7.3.2.1 More PstB is the ATP binding component Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
7.3.2.1 phosphate-specific transporter
-
 Escherichia coli
7.3.2.1 phosphate-specific transporter
-
 Mycobacterium tuberculosis
7.3.2.1 Pst
-
 Escherichia coli
7.3.2.1 Pst
-
 Mycobacterium tuberculosis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
7.3.2.1 24 80 pH 7.5, for 5 min, enzyme is heat-resistant Mycobacterium tuberculosis
7.3.2.1 37
-
 pH 7.5, for 5 min, substantial loss in the enzyme activity at temperatures above 37°C Escherichia coli
7.3.2.1 80
-
 pH 7.5 Mycobacterium tuberculosis
7.3.2.1 80
-
 pH 7.5, between 30 and 60 min of heat, maximum loss of enzyme activity, 60% of initial value Escherichia coli
7.3.2.1 80
-
 pH 7.5, incubating for 15 min, proteins are precipitated Escherichia coli
7.3.2.1 80
-
 pH 7.5, thermal denaturation is evident within 5 min Escherichia coli
7.3.2.1 80
-
 pH 7.5,incubating for 15 min, hardly shows any aggregation and for 1 h, loss 11% of its initial enzyme activity Mycobacterium tuberculosis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
7.3.2.1 5 10
-
 Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
7.3.2.1 0.4
-
 5'-p-fluorosulfonylbenzoyl adenosine pH 7.4, at 30°C Mycobacterium tuberculosis