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Literature summary extracted from

  • Arinc, E.; Celik, H.
    Biochemical characteristics of purified beef liver NADPH-cytochrome P450 reductase (2002), J. Biochem. Mol. Toxicol., 16, 286-297.
    View publication on PubMed

General Stability

EC Number General Stability Organism
1.6.2.4 20% glycerol stabilizes enzyme Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.048
-
ferricytochrome c pH 7.7 Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.2.4 membrane
-
Bos taurus 16020
-
1.6.2.4 membrane
-
Ovis aries 16020
-
1.6.2.4 microsome
-
Bos taurus
-
-
1.6.2.4 microsome
-
Ovis aries
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.2.4 76000
-
SDS-PAGE Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.2.4 2 ferricytochrome P450 + NADPH Bos taurus component of the cytochrome P450 dependent monooxygenase system 2 ferrocytochrome P450 + NADP+ + H+
-
?
1.6.2.4 2 ferricytochrome P450 + NADPH Ovis aries component of the cytochrome P450 dependent monooxygenase system 2 ferrocytochrome P450 + NADP+ + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Bos taurus
-
-
-
1.6.2.4 Ovis aries
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4
-
Bos taurus
1.6.2.4
-
Ovis aries

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 liver
-
Bos taurus
-
1.6.2.4 lung
-
Ovis aries
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.6.2.4 14.5
-
purified enzyme with cytochrome c as substrate, pH 7.7 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 2 ferricytochrome c + NADPH
-
Bos taurus 2 ferrocytochrome c + NADP+ + H+
-
?
1.6.2.4 2 ferricytochrome c + NADPH
-
Ovis aries 2 ferrocytochrome c + NADP+ + H+
-
?
1.6.2.4 2 ferricytochrome P450 + NADPH component of the cytochrome P450 dependent monooxygenase system Bos taurus 2 ferrocytochrome P450 + NADP+ + H+
-
?
1.6.2.4 2 ferricytochrome P450 + NADPH component of the cytochrome P450 dependent monooxygenase system Ovis aries 2 ferrocytochrome P450 + NADP+ + H+
-
?
1.6.2.4 benzphetamine demethylation in the presence of added lipid Bos taurus ?
-
?
1.6.2.4 benzphetamine demethylation in the presence of added lipid Ovis aries ?
-
?
1.6.2.4 cocaine demethylation in the presence of added lipid Bos taurus ?
-
?
1.6.2.4 cocaine demethylation in the presence of added lipid Ovis aries ?
-
?

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 NADPH-cytochrome P450 reductase
-
Bos taurus
1.6.2.4 NADPH-cytochrome P450 reductase
-
Ovis aries
1.6.2.4 NADPH-ferrihemoprotein reductase
-
Bos taurus
1.6.2.4 NADPH-ferrihemoprotein reductase
-
Ovis aries
1.6.2.4 NADPH:ferrihemoprotein oxidoreductase
-
Bos taurus
1.6.2.4 NADPH:ferrihemoprotein oxidoreductase
-
Ovis aries

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.4 37
-
85% loss of activity within 1 h without added glycerol, 54% loss of activity within 1 h in the presence of 20% glycerol Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.6.2.4 7.4 7.8
-
Bos taurus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD together with FMN Bos taurus
1.6.2.4 FAD together with FMN Ovis aries
1.6.2.4 FMN together with FAD Bos taurus
1.6.2.4 FMN together with FAD Ovis aries
1.6.2.4 NADPH
-
Bos taurus
1.6.2.4 NADPH
-
Ovis aries