EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.2.1 | - |
Methanosarcina thermophila |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.2.1 | F179A | reduced catalytic efficiency with all substrates tested | Methanosarcina thermophila |
2.7.2.1 | L122A | reduced catalytic efficiency with all substrates tested | Methanosarcina thermophila |
2.7.2.1 | P232A | reduced catalytic efficiency with all substrates tested | Methanosarcina thermophila |
2.7.2.1 | V93A | improved catalytic efficiency with propionate and butyrate | Methanosarcina thermophila |
2.7.2.1 | V93G | improved catalytic efficiency with propionate and butyrate | Methanosarcina thermophila |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 0.0499 | - |
ATP | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 0.0506 | - |
ATP | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 0.0542 | - |
ATP | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 0.0576 | - |
ATP | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 0.0687 | - |
ATP | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 0.0713 | - |
ATP | wild-type, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 0.15 | - |
acetyl phosphate | mutant F179A, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 0.34 | - |
acetyl phosphate | wild-type, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 0.5 | - |
acetyl phosphate | mutant L122A, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 0.71 | - |
acetyl phosphate | mutant V93G, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 0.74 | - |
acetyl phosphate | mutant V93A, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 1.19 | - |
acetyl phosphate | mutant P232A, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 1.5 | - |
acetate | wild-type, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 6.2 | - |
propionate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 10.4 | - |
acetate | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 10.7 | - |
propionate | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 11 | - |
propionate | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 14.4 | - |
propionate | wild-type, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 19.5 | - |
acetate | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 21.4 | - |
acetate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 25 | - |
propionate | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 32 | - |
acetate | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 33.4 | - |
Butyrate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 39 | - |
Butyrate | wild-type, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 39.5 | - |
acetate | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 46 | - |
propionate | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 63 | - |
Butyrate | mutant V93G, pH 7.0 | Methanosarcina thermophila |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.2.1 | Methanosarcina thermophila | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.2.1 | ATP + acetate = ADP + acetyl phosphate | residue F179 is essential for catalysis | Methanosarcina thermophila |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.2.1 | acetyl phosphate + hydroxylamine | - |
Methanosarcina thermophila | acetyl hydroxamate + phosphate | - |
r | |
2.7.2.1 | ATP + acetate | - |
Methanosarcina thermophila | ADP + acetyl phosphate | - |
r | |
2.7.2.1 | ATP + butyrate | - |
Methanosarcina thermophila | ADP + butyryl phosphate | - |
r | |
2.7.2.1 | ATP + propionate | - |
Methanosarcina thermophila | ADP + propionyl phosphate | - |
r |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.2.1 | 0.18 | - |
Butyrate | wild-type, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 0.37 | - |
propionate | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 2.2 | - |
ATP | mutant F179A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 6.7 | - |
propionate | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 8.5 | - |
propionate | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 12 | - |
acetyl phosphate | mutant F179A, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 42.4 | - |
Butyrate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 132 | - |
ATP | mutant P232A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 150 | - |
ATP | mutant L122A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 218 | - |
propionate | wild-type, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 294 | - |
Butyrate | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 347 | - |
ATP | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 840 | - |
propionate | mutant V93G, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 844 | - |
ATP | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 1029 | - |
propionate | mutant V93A, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 1039 | - |
acetyl phosphate | mutant L122A, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 1055 | - |
ATP | wild-type, pH 7.0 | Methanosarcina thermophila | |
2.7.2.1 | 2396 | - |
acetyl phosphate | mutant P232A, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 2572 | - |
acetyl phosphate | mutant V93G, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 2680 | - |
acetyl phosphate | wild-type, pH 7.4 | Methanosarcina thermophila | |
2.7.2.1 | 3869 | - |
acetyl phosphate | mutant V93A, pH 7.4 | Methanosarcina thermophila |