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Literature summary extracted from

  • Ingram-Smith, C.; Gorrell, A.; Lawrence, S.H.; Iyer, P.; Smith, K.; Ferry, J.G.
    Characterization of the acetate binding pocket in the Methanosarcina thermophila acetate kinase (2005), J. Bacteriol., 187, 2386-2394.
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.7.2.1
-
Methanosarcina thermophila

Protein Variants

EC Number Protein Variants Comment Organism
2.7.2.1 F179A reduced catalytic efficiency with all substrates tested Methanosarcina thermophila
2.7.2.1 L122A reduced catalytic efficiency with all substrates tested Methanosarcina thermophila
2.7.2.1 P232A reduced catalytic efficiency with all substrates tested Methanosarcina thermophila
2.7.2.1 V93A improved catalytic efficiency with propionate and butyrate Methanosarcina thermophila
2.7.2.1 V93G improved catalytic efficiency with propionate and butyrate Methanosarcina thermophila

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.2.1 0.0499
-
ATP mutant V93A, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.0506
-
ATP mutant F179A, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.0542
-
ATP mutant P232A, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.0576
-
ATP mutant L122A, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.0687
-
ATP mutant V93G, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.0713
-
ATP wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.15
-
acetyl phosphate mutant F179A, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.34
-
acetyl phosphate wild-type, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.5
-
acetyl phosphate mutant L122A, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.71
-
acetyl phosphate mutant V93G, pH 7.4 Methanosarcina thermophila
2.7.2.1 0.74
-
acetyl phosphate mutant V93A, pH 7.4 Methanosarcina thermophila
2.7.2.1 1.19
-
acetyl phosphate mutant P232A, pH 7.4 Methanosarcina thermophila
2.7.2.1 1.5
-
acetate wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 6.2
-
propionate mutant V93A, pH 7.0 Methanosarcina thermophila
2.7.2.1 10.4
-
acetate mutant F179A, pH 7.0 Methanosarcina thermophila
2.7.2.1 10.7
-
propionate mutant L122A, pH 7.0 Methanosarcina thermophila
2.7.2.1 11
-
propionate mutant F179A, pH 7.0 Methanosarcina thermophila
2.7.2.1 14.4
-
propionate wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 19.5
-
acetate mutant L122A, pH 7.0 Methanosarcina thermophila
2.7.2.1 21.4
-
acetate mutant V93A, pH 7.0 Methanosarcina thermophila
2.7.2.1 25
-
propionate mutant V93G, pH 7.0 Methanosarcina thermophila
2.7.2.1 32
-
acetate mutant P232A, pH 7.0 Methanosarcina thermophila
2.7.2.1 33.4
-
Butyrate mutant V93A, pH 7.0 Methanosarcina thermophila
2.7.2.1 39
-
Butyrate wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 39.5
-
acetate mutant V93G, pH 7.0 Methanosarcina thermophila
2.7.2.1 46
-
propionate mutant P232A, pH 7.0 Methanosarcina thermophila
2.7.2.1 63
-
Butyrate mutant V93G, pH 7.0 Methanosarcina thermophila

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.1 Methanosarcina thermophila
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
2.7.2.1 ATP + acetate = ADP + acetyl phosphate residue F179 is essential for catalysis Methanosarcina thermophila

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.1 acetyl phosphate + hydroxylamine
-
Methanosarcina thermophila acetyl hydroxamate + phosphate
-
r
2.7.2.1 ATP + acetate
-
Methanosarcina thermophila ADP + acetyl phosphate
-
r
2.7.2.1 ATP + butyrate
-
Methanosarcina thermophila ADP + butyryl phosphate
-
r
2.7.2.1 ATP + propionate
-
Methanosarcina thermophila ADP + propionyl phosphate
-
r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.7.2.1 0.18
-
Butyrate wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 0.37
-
propionate mutant F179A, pH 7.0 Methanosarcina thermophila
2.7.2.1 2.2
-
ATP mutant F179A, pH 7.0 Methanosarcina thermophila
2.7.2.1 6.7
-
propionate mutant L122A, pH 7.0 Methanosarcina thermophila
2.7.2.1 8.5
-
propionate mutant P232A, pH 7.0 Methanosarcina thermophila
2.7.2.1 12
-
acetyl phosphate mutant F179A, pH 7.4 Methanosarcina thermophila
2.7.2.1 42.4
-
Butyrate mutant V93A, pH 7.0 Methanosarcina thermophila
2.7.2.1 132
-
ATP mutant P232A, pH 7.0 Methanosarcina thermophila
2.7.2.1 150
-
ATP mutant L122A, pH 7.0 Methanosarcina thermophila
2.7.2.1 218
-
propionate wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 294
-
Butyrate mutant V93G, pH 7.0 Methanosarcina thermophila
2.7.2.1 347
-
ATP mutant V93G, pH 7.0 Methanosarcina thermophila
2.7.2.1 840
-
propionate mutant V93G, pH 7.0 Methanosarcina thermophila
2.7.2.1 844
-
ATP mutant V93A, pH 7.0 Methanosarcina thermophila
2.7.2.1 1029
-
propionate mutant V93A, pH 7.0 Methanosarcina thermophila
2.7.2.1 1039
-
acetyl phosphate mutant L122A, pH 7.4 Methanosarcina thermophila
2.7.2.1 1055
-
ATP wild-type, pH 7.0 Methanosarcina thermophila
2.7.2.1 2396
-
acetyl phosphate mutant P232A, pH 7.4 Methanosarcina thermophila
2.7.2.1 2572
-
acetyl phosphate mutant V93G, pH 7.4 Methanosarcina thermophila
2.7.2.1 2680
-
acetyl phosphate wild-type, pH 7.4 Methanosarcina thermophila
2.7.2.1 3869
-
acetyl phosphate mutant V93A, pH 7.4 Methanosarcina thermophila