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Literature summary extracted from

  • Stoveken, T.; Kalscheuer, R.; Malkus, U.; Reichelt, R.; Steinbuchel, A.
    The wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase from Acinetobacter sp. strain ADP1: characterization of a novel type of acyltransferase (2005), J. Bacteriol., 187, 1369-1376.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.20 ammonium sulfate 45 mM, 10% increase in activity Acinetobacter baylyi ADP1
2.3.1.75 ammonium sulfate 45 mM, 10% increase in activtiy Acinetobacter baylyi ADP1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.22 gene atfA, expression of the soluble enzyme in Escherichia coli strain BL21(DE3) Acinetobacter sp.

General Stability

EC Number General Stability Organism
2.3.1.20 bovine serum albumin stabilizes, decrease in activity with buffers such as bis-Tris, HEPES, morpholinoethanesulfonic acid Acinetobacter baylyi ADP1
2.3.1.75 decrease in activity with buffers such as Tris, Bis-Tris, HEPES, morpholinoethanesulfonic acid Acinetobacter baylyi ADP1
2.3.1.75 highly sensitive to proteases, especially to metallorpoteases, proteolysis is inhibited by addition of a cocktail of protease inhibitors including EDTA Acinetobacter baylyi ADP1

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.20 CaCl2 5 mM, 30% inhibition Acinetobacter baylyi ADP1
2.3.1.20 CoA above 50 mM, about 70% inhibition above 300 mM Acinetobacter baylyi ADP1
2.3.1.20 MgCl2 5 mM, 17% inhibition Acinetobacter baylyi ADP1
2.3.1.20 MnCl2 5 mM, 30% inhibition Acinetobacter baylyi ADP1
2.3.1.75 CaCl2 5 mM, 30% inhibition Acinetobacter baylyi ADP1
2.3.1.75 MgCl2 5 mM, 17% inhibition Acinetobacter baylyi ADP1
2.3.1.75 MnCl2 5 mM, 30% inhibition Acinetobacter baylyi ADP1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.75 0.029
-
 palmitoyl-CoA pH 7.4, 35°C Acinetobacter baylyi ADP1

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.20 lipid particle
-
 Acinetobacter baylyi ADP1
-
-
2.3.1.22 lipid particle mainly Acinetobacter sp.
-
-
2.3.1.22 membrane small enzyme amout Acinetobacter sp. 16020
-
2.3.1.22 additional information determination of subcellular distribution, overview Acinetobacter sp.
-
-
2.3.1.75 cytoplasm partially Acinetobacter baylyi ADP1 5737
-
2.3.1.75 lipid droplet
-
 Acinetobacter baylyi ADP1 5811
-
2.3.1.75 membrane cytoplasm membrane Acinetobacter baylyi ADP1 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.1.20 38100
-
 2 * 38100, gel filtration Acinetobacter baylyi ADP1
2.3.1.20 94100
-
 gel filtration Acinetobacter baylyi ADP1
2.3.1.22 38000
-
 recombinant enzyme, gel filtration, monomeric form Acinetobacter sp.
2.3.1.22 53000
-
 1 * 53000, recombinant enzyme, SDS-PAGE Acinetobacter sp.
2.3.1.22 53000
-
 2 * 53000, recombinant enzyme, SDS-PAGE Acinetobacter sp.
2.3.1.22 94100
-
 recombinant enzyme, gel filtration, dimeric form Acinetobacter sp.
2.3.1.75 53000
-
 2 * 53000, SDS-PAGE Acinetobacter baylyi ADP1
2.3.1.75 94100
-
 gel filtration Acinetobacter baylyi ADP1

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.20 Acinetobacter baylyi ADP1 Q8GGG1 expression in Escherichia coli
-
2.3.1.22 Acinetobacter sp.
-
 gene atfA
-
2.3.1.22 Acinetobacter sp. ADP1
-
 gene atfA
-
2.3.1.75 Acinetobacter baylyi ADP1 Q8GGG1 bifunctional wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase, EC 2.3.1.75 and EC 2.3.1.20 resp., enzyme also has acyl-CoA:monoacylglycerol acyltransferase activity, expression in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.20 recombinant enzyme Acinetobacter baylyi ADP1
2.3.1.22 recombinant soluble enzyme from Escherichia coli strain BL21(DE3) by cation exchange, hydrophobic interaction, and anion exchange chromatography, 18.7fold Acinetobacter sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.22 0.118
-
 purified recombinant enzyme, substrates palmitoyl-CoA and 1,3-dipalmitoylglycerol Acinetobacter sp.
2.3.1.22 0.211
-
 purified recombinant enzyme, substrates palmitoyl-CoA and 1,2-dipalmitoylglycerol Acinetobacter sp.
2.3.1.75 2
-
 pH 7.4, 35°C Acinetobacter baylyi ADP1

Storage Stability

EC Number Storage Stability Organism
2.3.1.22 -70°C, purified recombinant enzyme, no significant loss after several months Acinetobacter sp.
2.3.1.75 -70°C, several months without significant loss of activity Acinetobacter baylyi ADP1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.20 acyl-CoA + 1-decanol at 48% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + 2-cyclohexylethanol at 45% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + 2-decanol at 39% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + 4-decanol at 15.6% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + cyclododecanol at 80% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + cyclohexandiol at 4.1% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + cyclohexanol at 32% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + cyclohexanone oxime at 5.2% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + hexadecanol
-
 Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + phenol at 4.1% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 acyl-CoA + phenylethanol at 99% of the activity with hexanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.20 additional information enzyme also has acyl-CoA-monoacylglycerol acyltransferase activity, sn-1 and sn-3 positions are accepted with higher specificity than sn-2 position Acinetobacter baylyi ADP1 ?
-
 ?
2.3.1.22 additional information the bifunctional enzyme catalyzes the reaction of EC 2.3.1.22, acyl coenzyme A:diacylglycerol acyltransferase, and of EC 2.3.1.75, wax ester synthase, overview, linear acyl acceptor specificity, C14-C18 acceptor substrates in the wax ester synthase reaction are preferred, overview Acinetobacter sp. ?
-
 ?
2.3.1.22 additional information the bifunctional enzyme catalyzes the reaction of EC 2.3.1.22, acyl coenzyme A:diacylglycerol acyltransferase, and of EC 2.3.1.75, wax ester synthase, overview, linear acyl acceptor specificity, C14-C18 acceptor substrates in the wax ester synthase reaction are preferred, overview Acinetobacter sp. ADP1 ?
-
 ?
2.3.1.22 palmitoyl-CoA + 1,2-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. CoA + tripalmitoylglycerol
-
 ?
2.3.1.22 palmitoyl-CoA + 1,2-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. ADP1 CoA + tripalmitoylglycerol
-
 ?
2.3.1.22 palmitoyl-CoA + 1,3-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. CoA + tripalmitoylglycerol
-
 ?
2.3.1.22 palmitoyl-CoA + 1,3-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. ADP1 CoA + tripalmitoylglycerol
-
 ?
2.3.1.22 palmitoyl-CoA + 2,3-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. CoA + tripalmitoylglycerol
-
 ?
2.3.1.22 palmitoyl-CoA + 2,3-dipalmitoylglycerol i.e. 1,2-dipalmitin Acinetobacter sp. ADP1 CoA + tripalmitoylglycerol
-
 ?
2.3.1.75 1-decanol + palmitoyl-CoA 48% the rate of hexacedanol Acinetobacter baylyi ADP1 decyl palmitate + CoA
-
 ?
2.3.1.75 2-cyclohexylethanol + palmitoyl-CoA 44.8% the rate of hexacedanol Acinetobacter baylyi ADP1 2-cyclohexylethyl palmitate + CoA
-
 ?
2.3.1.75 2-decanol + palmitoyl-CoA 38.8% the rate of hexacedanol Acinetobacter baylyi ADP1 dec-2-yl palmitate + CoA
-
 ?
2.3.1.75 4-decanol + palmitoyl-CoA 15.6% the rate of hexacedanol Acinetobacter baylyi ADP1 4-decyl palmitate + CoA
-
 ?
2.3.1.75 cyclododecanol + palmitoyl-CoA 79.7% the rate of hexacedanol Acinetobacter baylyi ADP1 cyclododecyl palmitate + CoA
-
 ?
2.3.1.75 cyclohexandiol + palmitoyl-CoA 4.1% the rate of hexacedanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.75 cyclohexanol + palmitoyl-CoA 32% the rate of hexacedanol Acinetobacter baylyi ADP1 cyclohexyl palmitate + CoA
-
 ?
2.3.1.75 cyclohexanone oxime + palmitoyl-CoA 5.2% the rate of hexacedanol Acinetobacter baylyi ADP1 ? + CoA
-
 ?
2.3.1.75 hexadecanol + palmitoyl-CoA
-
 Acinetobacter baylyi ADP1 hexadecyl palmitate + CoA
-
 ?
2.3.1.75 additional information no substrate: resorcinol Acinetobacter baylyi ADP1 ?
-
 ?
2.3.1.75 phenol + palmitoyl-CoA 4.1% the rate of hexacedanol Acinetobacter baylyi ADP1 phenyl palmitate + CoA
-
 ?
2.3.1.75 phenylethanol + palmitoyl-CoA 99% the rate of hexacedanol Acinetobacter baylyi ADP1 phenylethyl palmitate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.1.20 dimer 2 * 38100, gel filtration Acinetobacter baylyi ADP1
2.3.1.22 dimer 2 * 53000, recombinant enzyme, SDS-PAGE Acinetobacter sp.
2.3.1.22 monomer 1 * 53000, recombinant enzyme, SDS-PAGE Acinetobacter sp.
2.3.1.75 dimer 2 * 53000, SDS-PAGE Acinetobacter baylyi ADP1

Synonyms

EC Number Synonyms Comment Organism
2.3.1.22 acyl coenzyme A:diacylglycerol acyltransferase
-
 Acinetobacter sp.
2.3.1.22 additional information cf. EC 2.3.1.75 Acinetobacter sp.
2.3.1.22 wax ester synthase/acyl coenzyme A:diacylglycerol acyltransferase
-
 Acinetobacter sp.
2.3.1.22 WS/DGAT
-
 Acinetobacter sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.22 35
-
 assay at Acinetobacter sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.20 40 45
-
 Acinetobacter baylyi ADP1
2.3.1.75 40 45
-
 Acinetobacter baylyi ADP1
2.3.1.75 65
-
 no activity above Acinetobacter baylyi ADP1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.20 4 40
-
 Acinetobacter baylyi ADP1
2.3.1.75 4 40 stable Acinetobacter baylyi ADP1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.20 8
-
 phosphate buffer Acinetobacter baylyi ADP1
2.3.1.22 8
-
 in phosphate buffer Acinetobacter sp.
2.3.1.75 8
-
 phosphate buffer Acinetobacter baylyi ADP1