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Literature summary extracted from

  • Fernandez-Murga, M.L.; Gil-Ortiz, F.; Llacer, J.L.; Rubio, V.
    Arginine biosynthesis in Thermotoga maritima: characterization of the arginine-sensitive N-acetyl-L-glutamate kinase (2004), J. Bacteriol., 186, 6142-6149.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.2.8 L-arginine pH and temperature dependent, sigmoidal dependence on concentration of arginine, Hill coefficient of 4, 1 mM, 37°C, 95% inhibition Thermotoga maritima
2.7.2.8 L-arginine methyl ester 1 mM, 80% inhibition Thermotoga maritima
2.7.2.8 additional information not inhibitory: D-arginine, agmatine, citrulline, L-canavanine, L-lysine, L-ornithine, guanidinium ions, urea at 1 mM, 37°C Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.2.8 30341
-
 6 * 30341, MALDI-TOF MS, 6 * 30344, calculated Thermotoga maritima
2.7.2.8 30344
-
 6 * 30341, MALDI-TOF MS, 6 * 30344, calculated Thermotoga maritima
2.7.2.8 180000
-
 gel filtration Pseudomonas aeruginosa
2.7.2.8 180000
-
 gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
2.7.2.8 Pseudomonas aeruginosa
-
 recombinant enzyme
-
2.7.2.8 Thermotoga maritima
-
 recombinant enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.2.8 recombinant enzyme Thermotoga maritima

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.2.8 45
-
 37°C, pH 7.5 Thermotoga maritima
2.7.2.8 130
-
 37°C, pH 7.5 Pseudomonas aeruginosa
2.7.2.8 677
-
 80°C, pH 7.5 Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Pseudomonas aeruginosa ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?
2.7.2.8 ATP + N-acetyl-L-glutamate
-
 Thermotoga maritima ADP + N-acetyl-L-glutamate 5-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.2.8 hexamer 6 * 30341, MALDI-TOF MS, 6 * 30344, calculated Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
2.7.2.8 NagK
-
 Pseudomonas aeruginosa
2.7.2.8 NagK
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.2.8 80
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.2.8 70
-
 30 min, complete inactivation Pseudomonas aeruginosa
2.7.2.8 70
-
 1 h, 100% residual activity Thermotoga maritima
2.7.2.8 80
-
 1 h, 80% residual activity Thermotoga maritima
2.7.2.8 85
-
 1 h, 60% residual activity Thermotoga maritima