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Literature summary extracted from

  • Vandenende, C.S.; Vlasschaert, M.; Seah, S.Y.K.
    Functional characterization of an aminotransferase required for pyoverdine siderophore biosynthesis in Pseudomonas aeruginosa PAO1 (2004), J. Bacteriol., 186, 5596-5602.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.46 gene pvdH, DNA sequence determination and analysis, subcloning and expression in Escherichia coli, overexpression in enzyme-knockout Pseudomonas aeruginosa strain PAO1 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.46 additional information
-
additional information steady-state kinetic analysis, enzyme shows a ping-pong kinetic mechanism Pseudomonas aeruginosa
2.6.1.46 0.18
-
2-oxoglutarate pH 7.5, 25°C, recombinant enzyme Pseudomonas aeruginosa
2.6.1.46 0.26
-
L-2,4-diaminobutanoate pH 7.5, 25°C, with 2-oxoglutarate, recombinant enzyme Pseudomonas aeruginosa
2.6.1.46 0.29
-
L-2,4-diaminobutanoate pH 7.5, 25°C, with pyruvate, recombinant enzyme Pseudomonas aeruginosa
2.6.1.46 6.2
-
pyruvate pH 7.5, 25°C, recombinant enzyme Pseudomonas aeruginosa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.6.1.46 additional information iron-limiting conditions induce pyoverdine siderophore biosynthesis including enzyme activity Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.46 50000
-
4 * 50000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa
2.6.1.46 183000
-
recombinant enzyme, gel filtration Pseudomonas aeruginosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.46 additional information Pseudomonas aeruginosa enzyme is involved in biosynthesis of the pyoverdine siderophore ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.46 Pseudomonas aeruginosa
-
gene pvdH
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.46 221fold, recombinantly overexpressed enzyme from Pseudomonas aeruginosa strain PAO1 by anion exchange chromatography and gel filtration to 90% purity Pseudomonas aeruginosa

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.46 L-2,4-diaminobutanoate + pyruvate = L-aspartate 4-semialdehyde + L-alanine ping-pong kinetic mechanism Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.46 2.21
-
purified recombinant enzyme, coupled assay with homoserine dehydrogenase from Saccharomyces cerevisiae Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.46 L-2,4-diaminobutanoate + 2-oxoglutarate 2-oxoglutarate is the best amino donor substrate Pseudomonas aeruginosa L-aspartate 4-semialdehyde + L-glutamate
-
r
2.6.1.46 L-2,4-diaminobutanoate + pyruvate specificty for pyruvate is 41fold lower compared to 2-oxoglutarate Pseudomonas aeruginosa L-aspartate 4-semialdehyde + L-alanine
-
r
2.6.1.46 additional information enzyme is involved in biosynthesis of the pyoverdine siderophore Pseudomonas aeruginosa ?
-
?
2.6.1.46 additional information no activity with 2-oxobutyrate as amino acceptor Pseudomonas aeruginosa ?
-
?

Subunits

EC Number Subunits Comment Organism
2.6.1.46 tetramer 4 * 50000, recombinant enzyme, SDS-PAGE Pseudomonas aeruginosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.46 25
-
assay at Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.46 1.39
-
pyruvate pH 7.5, 25°C, recombinant enzyme Pseudomonas aeruginosa
2.6.1.46 1.39
-
2-oxoglutarate pH 7.5, 25°C, recombinant enzyme Pseudomonas aeruginosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.46 7.5
-
assay at Pseudomonas aeruginosa

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.46 pyridoxal 5'-phosphate
-
Pseudomonas aeruginosa