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Literature summary extracted from

  • Chang, H.K.; Mohseni, P.; Zylstra, G.J.
    Characterization and regulation of the genes for a novel anthranilate 1,2-dioxygenase from Burkholderia cepacia DBO1 (2003), J. Bacteriol., 185, 5871-5881.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.1 expressed in Escherichia coli TOP10F' Burkholderia cepacia

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.12.1 18900
-
 AndAd, product of ORF4, calculated from deduced amino acid sequence Burkholderia cepacia
1.14.12.1 48000
-
 AndAc, product of ORF3, calculated from deduced amino acid sequence Burkholderia cepacia

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.1 anthranilic acid + alanine + O2 Burkholderia cepacia involved in the tryptophan degradation pathway catechol + CO2 + NH3 + ?
-
 ?
1.14.12.1 anthranilic acid + alanine + O2 Burkholderia cepacia DBO1 / ATCC 29424 involved in the tryptophan degradation pathway catechol + CO2 + NH3 + ?
-
 ?
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O Burkholderia cepacia involved in the tryptophan degradation pathway catechol + CO2 + NAD(P)+ + NH3
-
 ?
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O Burkholderia cepacia DBO1 / ATCC 29424 involved in the tryptophan degradation pathway catechol + CO2 + NAD(P)+ + NH3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.1 Burkholderia cepacia Q84BZ2 small subunit; three component type of enzyme
-
1.14.12.1 Burkholderia cepacia Q84BZ3 large subunit; three component type of enzyme
-
1.14.12.1 Burkholderia cepacia DBO1 / ATCC 29424 Q84BZ2 small subunit; three component type of enzyme
-
1.14.12.1 Burkholderia cepacia DBO1 / ATCC 29424 Q84BZ3 large subunit; three component type of enzyme
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.1 anthranilic acid + alanine + O2 involved in the tryptophan degradation pathway Burkholderia cepacia catechol + CO2 + NH3 + ?
-
 ?
1.14.12.1 anthranilic acid + alanine + O2 involved in the tryptophan degradation pathway Burkholderia cepacia DBO1 / ATCC 29424 catechol + CO2 + NH3 + ?
-
 ?
1.14.12.1 anthranilic acid + alanine + O2
-
 Burkholderia cepacia ?
-
 ?
1.14.12.1 anthranilic acid + alanine + O2
-
 Burkholderia cepacia DBO1 / ATCC 29424 ?
-
 ?
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O
-
 Burkholderia cepacia catechol + CO2 + NAD(P)+ + NH3
-
 ?
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O involved in the tryptophan degradation pathway Burkholderia cepacia catechol + CO2 + NAD(P)+ + NH3
-
 ?
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O
-
 Burkholderia cepacia DBO1 / ATCC 29424 catechol + CO2 + NAD(P)+ + NH3
-
 ?
1.14.12.1 anthranilic acid + NAD(P)H + O2 + H2O involved in the tryptophan degradation pathway Burkholderia cepacia DBO1 / ATCC 29424 catechol + CO2 + NAD(P)+ + NH3
-
 ?
1.14.12.1 benzoate + NAD(P)H + O2 + H2O poor substrate Burkholderia cepacia benzoate dihydrodiol + ?
-
 ?
1.14.12.1 benzoate + NAD(P)H + O2 + H2O poor substrate Burkholderia cepacia DBO1 / ATCC 29424 benzoate dihydrodiol + ?
-
 ?
1.14.12.1 salicylic acid + NAD(P)H + O2 + H2O
-
 Burkholderia cepacia ?
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.1 iron-sulfur centre proximal Rieske [2Fe-2S] center Burkholderia cepacia
1.14.12.1 NADH
-
 Burkholderia cepacia
1.14.12.1 NADPH
-
 Burkholderia cepacia