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Literature summary extracted from

  • Taylor, A.B.; Benglis, D.M., Jr.; Dhandayuthapani, S.; Hart, P.J.
    Structure of Mycobacterium tuberculosis methionine sulfoxide reductase A in complex with protein-bound methionine (2003), J. Bacteriol., 185, 4119-4126.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.4.11 overexpression of N-terminally 10His-tagged enzyme MsrA in Escherichia coli strain Bl21(DE3) Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.8.4.11 single crystals of recombinant N-terminally 10His-tagged enzyme MsrA complexed with protein-bound methionine, hanging drop method, 30 mg/ml protein in 25 mM Tris-HCl, pH 8.0, 1 mM EDTA, 1 mM tris(carboxyethyl)phosphine hydrochloride, precipitant solution contains 2.0 M sodium formate, 0.1 M sodium citrate, pH 6.0, 4°C, 1 week, prior to data collection, crystals are soaked in 6.3 M sodium formate, 0.1 M sodium citrate, pH 6.0, for 2 min, and are flash-cooled, X-ray diffraction structure determination and analysis at 1.5 A resolution, polycrystalline clusters are obtained by sitting drop vapor diffusion method Mycobacterium tuberculosis

Protein Variants

EC Number Protein Variants Comment Organism
1.8.4.11 additional information msrA gene disruption leads to loss of pathogenicity of the bacterium due to loss of ability to colonize host cells in humans Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.4.11 L-methionine (S)-sulfoxide + thioredoxin Mycobacterium tuberculosis
-
 L-methionine + thioredoxin disulfide + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.8.4.11 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.4.11 recombinant N-terminally 10His-tagged enzyme MsrA from Escherichia coli strain Bl21(DE3) by nickel affinity chromatography Mycobacterium tuberculosis

Reaction

EC Number Reaction Comment Organism Reaction ID
1.8.4.11 L-methionine (S)-sulfoxide + thioredoxin = L-methionine + thioredoxin disulfide + H2O catalytic mechanism of MsrA, active site structure, modeling of protein-bound methionine sulfoxide recognition and repair from the crystal structure Mycobacterium tuberculosis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.4.11 L-methionine (S)-sulfoxide + thioredoxin
-
 Mycobacterium tuberculosis L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 L-methionine (S)-sulfoxide + thioredoxin absolute specificity for the S-form Mycobacterium tuberculosis L-methionine + thioredoxin disulfide + H2O
-
 ?
1.8.4.11 L-methionine (S)-sulfoxide + thioredoxin enzyme MsrA, absolute specificity for the S-form Mycobacterium tuberculosis L-methionine + thioredoxin disulfide + H2O
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.8.4.11 methionine sulfoxide reductase A
-
 Mycobacterium tuberculosis
1.8.4.11 MsrA
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.4.11 thioredoxin
-
 Mycobacterium tuberculosis