EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.1.85 | gene sgbH, expression in strain BL21(DE3) as N-terminally His10-tagged protein | Escherichia coli K-12 |
5.1.3.22 | expression in Escherichia coli | Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
4.1.1.85 | Mg2+ | - |
Escherichia coli K-12 |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.85 | 3-dehydro-L-gulonate 6-phosphate + H+ | Escherichia coli K-12 | SgaH/UlaD and SgbH | L-xylulose 5-phosphate + CO2 | - |
? | |
4.1.1.85 | additional information | Escherichia coli K-12 | enzyme catalyzes one step in the L-ascorbate utilization pathway, L-ascorbate is converted to L-xylulose, overview | ? | - |
? | |
5.1.3.22 | L-ribulose 5-phosphate | Escherichia coli | along with EC 4.1.1.83, 3-dehydro-L-gulonate-6-phosphate decarboxylase, this enzyme is involved in a pathway for the utilization of L-ascorbate by Escherichia coli | L-xylulose 5-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.1.85 | Escherichia coli K-12 | - |
gene ulaD or sgaH encoded in the yia-sga operon, and gene sgbH encoded in the yia-sgb operon | - |
5.1.3.22 | Escherichia coli | - |
K-12 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
4.1.1.85 | recombinant N-terminally His10-tagged SgbH from strain BL21(DE3) to homogeneity, the His-tag is removed by thrombin | Escherichia coli K-12 |
5.1.3.22 | His-tagged protein | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
4.1.1.85 | additional information | - |
- |
Escherichia coli K-12 |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.1.85 | 3-dehydro-L-gulonate 6-phosphate + H+ | SgaH/UlaD and SgbH | Escherichia coli K-12 | L-xylulose 5-phosphate + CO2 | - |
? | |
4.1.1.85 | additional information | enzyme catalyzes one step in the L-ascorbate utilization pathway, L-ascorbate is converted to L-xylulose, overview | Escherichia coli K-12 | ? | - |
? | |
4.1.1.85 | additional information | enzymes SgaH/UlaD and SgbH are not active with 3-dehydro-L-gulonate | Escherichia coli K-12 | ? | - |
? | |
5.1.3.22 | L-ribulose 5-phosphate | - |
Escherichia coli | L-xylulose 5-phosphate | - |
? | |
5.1.3.22 | L-ribulose 5-phosphate | along with EC 4.1.1.83, 3-dehydro-L-gulonate-6-phosphate decarboxylase, this enzyme is involved in a pathway for the utilization of L-ascorbate by Escherichia coli | Escherichia coli | L-xylulose 5-phosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.1.85 | 3-keto-L-gulonate 6-phosphate decarboxylase | - |
Escherichia coli K-12 |
4.1.1.85 | SgaH | - |
Escherichia coli K-12 |
4.1.1.85 | SgbH | - |
Escherichia coli K-12 |
4.1.1.85 | UlaD | - |
Escherichia coli K-12 |
5.1.3.22 | L-xylulose 5-phosphate 3-epimerase | - |
Escherichia coli |
5.1.3.22 | SgaU | - |
Escherichia coli |
5.1.3.22 | UlaE | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
4.1.1.85 | 25 | - |
assay at | Escherichia coli K-12 |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
4.1.1.85 | 51 | - |
3-dehydro-L-gulonate 6-phosphate | SgaH, pH 7.5, 25°C | Escherichia coli K-12 | |
4.1.1.85 | 64 | - |
3-dehydro-L-gulonate 6-phosphate | SgbH, pH 7.5, 25°C | Escherichia coli K-12 |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
4.1.1.85 | 7.5 | - |
assay at | Escherichia coli K-12 |