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Literature summary extracted from

  • Bell, S.G.; Chen, X.; Sowden, R.J.; Xu, F.; Williams, J.N.; Wong, L.L.; Rao, Z.
    Molecular recognition in (+)-alpha-pinene oxidation by cytochrome P450cam (2003), J. Am. Chem. Soc., 125, 705-714.
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.15.1 synthesis selective enzymatic oxidation of (+)-alpha-pinene to verbenol, verbenone, or myrtenol by enzyme mutants Pseudomonas putida

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.14.15.1 mutant Y96F/F87W/V247L, binding of substrate (+)-alpha-pinene in two orientations related by rotation of the molecule Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
1.14.15.1 F87A/Y96F enhanced binding and oxidation of (+)-alpha-pinene Pseudomonas putida
1.14.15.1 F87L/Y96F enhanced binding and oxidation of (+)-alpha-pinene Pseudomonas putida
1.14.15.1 F87W/Y96F enhanced binding and oxidation of (+)-alpha-pinene Pseudomonas putida
1.14.15.1 F87W/Y96F/L244A enhanced binding and oxidation of (+)-alpha-pinene, production of 86% (+)-cis-verbenol + 5% (+)-verbenone Pseudomonas putida
1.14.15.1 F87W/Y96F/L244A/V247L enhanced binding and oxidation of (+)-alpha-pinene Pseudomonas putida
1.14.15.1 F87W/Y96F/V247L enhanced binding and oxidation of (+)-alpha-pinene Pseudomonas putida
1.14.15.1 Y96F enhanced binding and oxidation of (+)-alpha-pinene Pseudomonas putida
1.14.15.1 Y96F/L244A/V247L enhanced binding and oxidation of (+)-alpha-pinene, production of 55% (+)-cis-verbenol + 32% (+)-verbenone Pseudomonas putida
1.14.15.1 Y96F/V247L enhanced binding and oxidation of (+)-alpha-pinene Pseudomonas putida

Organism

EC Number Organism UniProt Comment Textmining
1.14.15.1 Pseudomonas putida P00183
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.15.1 (+)-alpha-pinene + putidaredoxin + O2
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 Pseudomonas putida (+)-cis-verbenol + (+)-myrtenol + (+)-verbenone + oxidized putidaredoxin + H2O
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