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Literature summary extracted from

  • Nandi, D.; Lightcap, E.S.; Koo, Y.K.; Lu, X.; Quancard, J.; Silverman, R.B.
    Purification and inactivation of 3-hydroxyanthranilic acid 3,4-dioxygenase from beef liver (2003), Int. J. Biochem. Cell Biol., 35, 1085-1097.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.6 6-chloro-3-hydroxyanthranilic acid 5-20 mM, loss of enzymatic activity as a function of the inhibitor concentration Bos taurus
1.13.11.6 anthranilic acid competitive inhibition Bos taurus
1.13.11.6 quinolinic acid competitive inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.6 16 19 3-Hydroxyanthranilate
-
 Bos taurus
1.13.11.6 36 57 3-hydroxy-4-methylanthranilic acid
-
 Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.6 32520
-
 PAGE in nondenaturing conditions, pI 4.98 enzyme Bos taurus
1.13.11.6 32570
-
 PAGE in nondenaturing conditions, pI 5.6 enzyme Bos taurus
1.13.11.6 33000
-
 SDS-PAGE, for the two active enzyme solutions obtained from hydroxyapatite column Bos taurus
1.13.11.6 33000 34000 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.6 3-hydroxyanthranilate + O2 Bos taurus
-
 2-amino-3-carboxymuconate semialdehyde
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.6 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.6 succesive size exclusion and affinity columns Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.6 liver
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.6 115.7
-
 last purification step Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.6 3-hydroxy-4-methylanthranilic acid + O2
-
 Bos taurus ?
-
 ?
1.13.11.6 3-hydroxyanthranilate + O2
-
 Bos taurus 2-amino-3-carboxymuconate semialdehyde
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.6 monomer 1 * 33000-34000, gel filtration Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.13.11.6
-
 4 stable for a month, in Tris-maleate buffer, pH 6.5 Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.6 6.9 7.4
-
 Bos taurus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.13.11.6 1.8
-
 quinolinic acid for the pI 5.6 enzyme Bos taurus
1.13.11.6 2
-
 anthranilic acid for the pI 4.98 enzyme Bos taurus
1.13.11.6 4.9
-
 anthranilic acid for the pI 4.98 enzyme Bos taurus
1.13.11.6 6.5
-
 anthranilic acid for the pI 5.6 enzyme Bos taurus
1.13.11.6 12
-
 6-chloro-3-hydroxyanthranilic acid
-
 Bos taurus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.6 Bos taurus chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4
-
 4.98
1.13.11.6 Bos taurus chromatofocusing in PBE 94 gel exchanger and polybuffer 74, pH 4-7.4
-
 5.6