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Literature summary extracted from

  • Bailey, A.M.; Mahapatra, S.; Brennan, P.J.; Crick, D.C.
    Identification, cloning, purification, and enzymatic characterization of Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate synthase (2002), Glycobiology, 12, 813-820.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.2.1.7 expression in Escherichia coli Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.2.1.7 0.0061
-
 D-glyceraldehyde 3-phosphate pH 7.9, 37°C Mycobacterium tuberculosis
2.2.1.7 0.04
-
 pyruvate pH 7.9, 37°C Mycobacterium tuberculosis
2.2.1.7 0.12
-
 D-erythrose 4-phosphate pH 7.9, 37°C Mycobacterium tuberculosis
2.2.1.7 4.5
-
 L-glyceraldehyde pH 7.9, 37°C Mycobacterium tuberculosis
2.2.1.7 5.6
-
 D-glyceraldehyde pH 7.9, 37°C Mycobacterium tuberculosis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.2.1.7 cytosol
-
 Mycobacterium tuberculosis 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.2.1.7 Mg2+ divalent cation required, optimal concentration: 0.5-1 mM Mycobacterium tuberculosis
2.2.1.7 Mn2+ divalent cation required Mycobacterium tuberculosis
2.2.1.7 Zn2+ divalent cation required, supports activity to a lower level than Mn2+ or Mg2+ Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.2.1.7 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.2.1.7
-
 Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.2.1.7 pyruvate + D-erythrose 4-phosphate
-
 Mycobacterium tuberculosis 1-deoxy-D-fructose 6-phosphate + CO2
-
 ?
2.2.1.7 pyruvate + D-glyceraldehyde
-
 Mycobacterium tuberculosis 1-deoxy-D-xylulose + CO2
-
 ?
2.2.1.7 pyruvate + D-glyceraldehyde 3-phosphate
-
 Mycobacterium tuberculosis 1-deoxy-D-xylulose 5-phosphate + CO2
-
 ?
2.2.1.7 pyruvate + D-ribose 5-phosphate
-
 Mycobacterium tuberculosis 1-deoxy-D-sedoheptulose 7-phosphate + CO2
-
 ?
2.2.1.7 pyruvate + L-glyceraldehyde
-
 Mycobacterium tuberculosis 1-deoxy-D-xylulose + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.2.1.7 DXS
-
 Mycobacterium tuberculosis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.2.1.7 7 8
-
 Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.2.1.7 thiamine diphosphate required, Kd: 0.001 M, optimal concentration: 0.005-0.01 mM Mycobacterium tuberculosis