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Literature summary extracted from

  • Zilberman-Peled, B.; Benhar, I.; Coon, S.L.; Ron, B.; Gothilf, Y.
    Duality of serotonin-N-acetyltransferase in the gilthead seabream (Sparus aurata): molecular cloning and characterization of recombinant enzymes (2004), Gen. Comp. Endocrinol., 138, 139-147.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.87 phosphate optimal activity at 0.1 M Sparus aurata
2.3.1.87 phosphate optimal activity at 0.3 M Sparus aurata

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.87
-
 Sparus aurata

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.87 Melatonin IC50: 0.26 mM Sparus aurata
2.3.1.87 additional information no inhibition by serotonin or tryptamin above 1 mM. No inhibition by melatonin Sparus aurata
2.3.1.87 N-acetylserotonin IC50: 0.68 mM; very slightly Sparus aurata
2.3.1.87 serotonin above 1 mM Sparus aurata
2.3.1.87 tryptamine above 1 mM Sparus aurata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.87 0.05
-
 serotonin
-
 Sparus aurata
2.3.1.87 0.1
-
 tryptamine
-
 Sparus aurata
2.3.1.87 0.34
-
 Phenylethylamine
-
 Sparus aurata
2.3.1.87 0.62
-
 acetyl-CoA
-
 Sparus aurata
2.3.1.87 1.11
-
 acetyl-CoA pH 6.8, 27°C Sparus aurata
2.3.1.87 1.25
-
 tyramine
-
 Sparus aurata
2.3.1.87 1.4
-
 tryptamine pH 6.8, 27°C Sparus aurata
2.3.1.87 2.05
-
 serotonin pH 6.8, 27°C Sparus aurata

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.87 NaCl optimal activity at 0.5 M Sparus aurata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.87 additional information Sparus aurata AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin ?
-
 ?
2.3.1.87 additional information Sparus aurata AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.87 Sparus aurata Q68SL6
-
-
2.3.1.87 Sparus aurata Q68SL7
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.87 photoreceptor
-
 Sparus aurata
-
2.3.1.87 pineal gland
-
 Sparus aurata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.87 acetyl-CoA + phenylethylamine
-
 Sparus aurata CoA + N-acetyl-phenylethylamine
-
 ?
2.3.1.87 acetyl-CoA + serotonin
-
 Sparus aurata CoA + N-acetylserotonin
-
 ?
2.3.1.87 acetyl-CoA + serotonin AANAT2 acetylates serotonin about 10times faster than AANAT1 Sparus aurata CoA + N-acetylserotonin
-
 ?
2.3.1.87 acetyl-CoA + tryptamine
-
 Sparus aurata CoA + N-acetyltryptamine
-
 ?
2.3.1.87 acetyl-CoA + tyramine
-
 Sparus aurata CoA + N-acetyltyramine
-
 ?
2.3.1.87 additional information AANAT1 may carry out an as yet unknown function that involves acetylation of arylalkylamines other than serotonin Sparus aurata ?
-
 ?
2.3.1.87 additional information AANAT2 is responsible for the production of large amounts of melatonin that is released into the circulation and exerts an endocrine role Sparus aurata ?
-
 ?
2.3.1.87 additional information insignificant activity in presence of phenylethylamine or tyramine Sparus aurata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.87 AANAT1
-
 Sparus aurata
2.3.1.87 AANAT2
-
 Sparus aurata
2.3.1.87 serotonin-N-acetyltransferase
-
 Sparus aurata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.87 27
-
-
 Sparus aurata
2.3.1.87 36
-
-
 Sparus aurata

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.3.1.87
-
 40 0°C: about 55% of maximal activity, 47°C: about 50% of maximal activity Sparus aurata
2.3.1.87 20 47 20°C: about 40% of maximal activity, 47°C: about 35% of maximal activity Sparus aurata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.87 6.5
-
-
 Sparus aurata

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.3.1.87 0.26
-
 IC50: 0.26 mM Sparus aurata Melatonin
2.3.1.87 0.68
-
 IC50: 0.68 mM Sparus aurata N-acetylserotonin