EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | methylene blue | stimulates reduction of cytochrome c and myoglobin | Sus scrofa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | additional information | no inhibition of the nitric oxide reduction by cyanide, antimycin A, or diphenyleneiodonium ions | Sus scrofa | |
1.8.1.4 | N-ethylmaleimide | over 90% inhibition at 1 mM | Sus scrofa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.4 | 0.0005 | - |
NO | nitric oxide reduction, pH 7.5 | Sus scrofa | |
1.8.1.4 | 0.01 | - |
NADH | nitric oxide reduction, pH 7.5 | Sus scrofa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.8.1.4 | 55000 | - |
x * 55000, SDS-PAGE | Sus scrofa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.4 | additional information | Sus scrofa | the enzyme might play a role in modifying NO levels under specific cell conditions | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.4 | Sus scrofa | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.4 | further purification of the commercial preparation | Sus scrofa |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.8.1.4 | commercial preparation | - |
Sus scrofa | - |
1.8.1.4 | heart | - |
Sus scrofa | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.8.1.4 | additional information | - |
- |
Sus scrofa |
1.8.1.4 | 0.5 | - |
metmyoglobin reduction, in absence of methylene blue, pH 7.5 | Sus scrofa |
1.8.1.4 | 0.6 | - |
O2 reduction, pH 6.0 | Sus scrofa |
1.8.1.4 | 0.8 | - |
cytochrome c reduction, in absence of methylene blue, pH 7.5 | Sus scrofa |
1.8.1.4 | 1.7 | - |
lipoic acid reduction, pH 6.0 | Sus scrofa |
1.8.1.4 | 4.5 | - |
metmyoglobin reduction, in presence of methylene blue, pH 7.5 | Sus scrofa |
1.8.1.4 | 6.4 | - |
cytochrome c reduction, in presence of methylene blue, pH 7.5 | Sus scrofa |
1.8.1.4 | 7.2 | - |
2,6-dichlorophenolindophenol reduction, pH 6.0 | Sus scrofa |
1.8.1.4 | 154 | - |
lipoamide reduction, pH 6.0 | Sus scrofa |
1.8.1.4 | 210 | - |
nitric oxide reduction, pH 6.0 | Sus scrofa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.4 | 2 ferricytochrome c + NADH | cytochrome c is a poor electron acceptor, only in presence of methylene blue the enzyme shows some activity | Sus scrofa | 2 ferrocytochrome c + NAD+ + H+ | - |
? | |
1.8.1.4 | metmyoglobin + NADH | myoglobin is a poor electron acceptor, only in presence of methylene blue the enzyme shows some activity | Sus scrofa | reduced myoglobin + NAD+ | - |
? | |
1.8.1.4 | additional information | the enzyme might play a role in modifying NO levels under specific cell conditions | Sus scrofa | ? | - |
? | |
1.8.1.4 | NADH + H+ + oxidized 2,6-dichlorophenolindophenol | - |
Sus scrofa | NAD+ + reduced 2,6-dichlorophenolindophenol | - |
? | |
1.8.1.4 | nitric oxide + NADH | - |
Sus scrofa | nitrate + NAD+ | - |
? | |
1.8.1.4 | O2 + NADH | - |
Sus scrofa | H2O2 + NAD+ | - |
? | |
1.8.1.4 | oxidized lipoamide + NADH | - |
Sus scrofa | reduced lipoamide + NAD+ | - |
? | |
1.8.1.4 | oxidized lipoic acid + NADH | - |
Sus scrofa | reduced lipoic acid + NAD+ | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.4 | ? | x * 55000, SDS-PAGE | Sus scrofa |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.4 | dihydrolipoamide dehydrogenase | - |
Sus scrofa |
1.8.1.4 | DLDH | - |
Sus scrofa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.1.4 | 5 | 6 | reduction of nitric oxide, lioamide, lipoic acid, and 2,6-dichlorophenolindophenol | Sus scrofa |
1.8.1.4 | 7.5 | - |
reduction of cytochrome c and myoglobin, oxidation of NADH | Sus scrofa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
1.8.1.4 | 5 | 8 | nitric oxide reduction | Sus scrofa |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | FAD | - |
Sus scrofa | |
1.8.1.4 | additional information | no activity with NADPH | Sus scrofa | |
1.8.1.4 | NADH | - |
Sus scrofa |