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Literature summary extracted from

  • Igamberdiev, A.U.; Bykova, N.V.; Ens, W.; Hill, R.D.
    Dihydrolipoamide dehydrogenase from porcine heart catalyzes NADH-dependent scavenging of nitric oxide (2004), FEBS Lett., 568, 146-150.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.8.1.4 methylene blue stimulates reduction of cytochrome c and myoglobin Sus scrofa

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.8.1.4 additional information no inhibition of the nitric oxide reduction by cyanide, antimycin A, or diphenyleneiodonium ions Sus scrofa
1.8.1.4 N-ethylmaleimide over 90% inhibition at 1 mM Sus scrofa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.1.4 0.0005
-
NO nitric oxide reduction, pH 7.5 Sus scrofa
1.8.1.4 0.01
-
NADH nitric oxide reduction, pH 7.5 Sus scrofa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.8.1.4 55000
-
x * 55000, SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.8.1.4 additional information Sus scrofa the enzyme might play a role in modifying NO levels under specific cell conditions ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.8.1.4 Sus scrofa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.1.4 further purification of the commercial preparation Sus scrofa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.8.1.4 commercial preparation
-
Sus scrofa
-
1.8.1.4 heart
-
Sus scrofa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.1.4 additional information
-
-
Sus scrofa
1.8.1.4 0.5
-
metmyoglobin reduction, in absence of methylene blue, pH 7.5 Sus scrofa
1.8.1.4 0.6
-
O2 reduction, pH 6.0 Sus scrofa
1.8.1.4 0.8
-
cytochrome c reduction, in absence of methylene blue, pH 7.5 Sus scrofa
1.8.1.4 1.7
-
lipoic acid reduction, pH 6.0 Sus scrofa
1.8.1.4 4.5
-
metmyoglobin reduction, in presence of methylene blue, pH 7.5 Sus scrofa
1.8.1.4 6.4
-
cytochrome c reduction, in presence of methylene blue, pH 7.5 Sus scrofa
1.8.1.4 7.2
-
2,6-dichlorophenolindophenol reduction, pH 6.0 Sus scrofa
1.8.1.4 154
-
lipoamide reduction, pH 6.0 Sus scrofa
1.8.1.4 210
-
nitric oxide reduction, pH 6.0 Sus scrofa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.1.4 2 ferricytochrome c + NADH cytochrome c is a poor electron acceptor, only in presence of methylene blue the enzyme shows some activity Sus scrofa 2 ferrocytochrome c + NAD+ + H+
-
?
1.8.1.4 metmyoglobin + NADH myoglobin is a poor electron acceptor, only in presence of methylene blue the enzyme shows some activity Sus scrofa reduced myoglobin + NAD+
-
?
1.8.1.4 additional information the enzyme might play a role in modifying NO levels under specific cell conditions Sus scrofa ?
-
?
1.8.1.4 NADH + H+ + oxidized 2,6-dichlorophenolindophenol
-
Sus scrofa NAD+ + reduced 2,6-dichlorophenolindophenol
-
?
1.8.1.4 nitric oxide + NADH
-
Sus scrofa nitrate + NAD+
-
?
1.8.1.4 O2 + NADH
-
Sus scrofa H2O2 + NAD+
-
?
1.8.1.4 oxidized lipoamide + NADH
-
Sus scrofa reduced lipoamide + NAD+
-
?
1.8.1.4 oxidized lipoic acid + NADH
-
Sus scrofa reduced lipoic acid + NAD+
-
?

Subunits

EC Number Subunits Comment Organism
1.8.1.4 ? x * 55000, SDS-PAGE Sus scrofa

Synonyms

EC Number Synonyms Comment Organism
1.8.1.4 dihydrolipoamide dehydrogenase
-
Sus scrofa
1.8.1.4 DLDH
-
Sus scrofa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.1.4 5 6 reduction of nitric oxide, lioamide, lipoic acid, and 2,6-dichlorophenolindophenol Sus scrofa
1.8.1.4 7.5
-
reduction of cytochrome c and myoglobin, oxidation of NADH Sus scrofa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.8.1.4 5 8 nitric oxide reduction Sus scrofa

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.1.4 FAD
-
Sus scrofa
1.8.1.4 additional information no activity with NADPH Sus scrofa
1.8.1.4 NADH
-
Sus scrofa