EC Number | Cloned (Comment) | Organism |
---|---|---|
2.5.1.22 | DNA sequence determination and analysis of gene SPMS, construction of expression vectors for recombinant expression in transgenic plants/mutants of genes ACAULIS5, i.e. ACL5, and SPMS | Arabidopsis thaliana |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.5.1.22 | additional information | the loss-of-function mutant of gene ACAULIS5 shows a severe defect in stem elongation, isolation of a T-DNA insertion mutant of gene SPMS, i.e. spms-1, showing decreased spermine levels but no obvious phenotypic alterations, an acl5-spms-1 double mutant contains no spermine but is fully viable as the wild-type and shows no phenotypic alterations under normal growth conditions, overview | Arabidopsis thaliana |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.22 | S-adenosylmethioninamine + spermidine | Arabidopsis thaliana | the enzyme, which is involved in the polyamine biosynthetic pathway in producing the final product spermine, is not essential for plant survival under normal growth conditions | 5'-methylthioadenosine + spermine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.5.1.22 | Arabidopsis thaliana | - |
ecotype Columbia Col-0, 2 genes: ACAULIS5, i.e. ACL5, and SPMS | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.5.1.22 | additional information | - |
polyamine content analysis of wild-type and transgenic plants | Arabidopsis thaliana |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.5.1.22 | S-adenosylmethioninamine + spermidine | - |
Arabidopsis thaliana | 5'-methylthioadenosine + spermine | - |
? | |
2.5.1.22 | S-adenosylmethioninamine + spermidine | the enzyme, which is involved in the polyamine biosynthetic pathway in producing the final product spermine, is not essential for plant survival under normal growth conditions | Arabidopsis thaliana | 5'-methylthioadenosine + spermine | - |
? |