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Literature summary extracted from

  • Imai, A.; Akiyama, T.; Kato, T.; Sato, S.; Tabata, S.; Yamamoto, K.T.; Takahashi, T.
    Spermine is not essential for survival of Arabidopsis (2004), FEBS Lett., 556, 148-152.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.22 DNA sequence determination and analysis of gene SPMS, construction of expression vectors for recombinant expression in transgenic plants/mutants of genes ACAULIS5, i.e. ACL5, and SPMS Arabidopsis thaliana

Protein Variants

EC Number Protein Variants Comment Organism
2.5.1.22 additional information the loss-of-function mutant of gene ACAULIS5 shows a severe defect in stem elongation, isolation of a T-DNA insertion mutant of gene SPMS, i.e. spms-1, showing decreased spermine levels but no obvious phenotypic alterations, an acl5-spms-1 double mutant contains no spermine but is fully viable as the wild-type and shows no phenotypic alterations under normal growth conditions, overview Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.22 S-adenosylmethioninamine + spermidine Arabidopsis thaliana the enzyme, which is involved in the polyamine biosynthetic pathway in producing the final product spermine, is not essential for plant survival under normal growth conditions 5'-methylthioadenosine + spermine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.22 Arabidopsis thaliana
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 ecotype Columbia Col-0, 2 genes: ACAULIS5, i.e. ACL5, and SPMS
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Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.22 additional information
-
 polyamine content analysis of wild-type and transgenic plants Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.22 S-adenosylmethioninamine + spermidine
-
 Arabidopsis thaliana 5'-methylthioadenosine + spermine
-
 ?
2.5.1.22 S-adenosylmethioninamine + spermidine the enzyme, which is involved in the polyamine biosynthetic pathway in producing the final product spermine, is not essential for plant survival under normal growth conditions Arabidopsis thaliana 5'-methylthioadenosine + spermine
-
 ?