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Literature summary extracted from

  • Bittel, C.; Tabares, L.C.; Armesto, M.; Carrillo, N.; Cortez, N.
    The oxidant-responsive diaphorase of Rhodobacter capsulatus is a ferredoxin (flavodoxin)-NADP(H) reductase (2003), FEBS Lett., 553, 408-412.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.19.1.1 flavodoxin cytochrome c reductase activity of FPR is stimulated three times by the addition of flavodoxin Rhodobacter capsulatus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.18.1.2 gene fpr, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli Rhodobacter capsulatus
1.19.1.1 expression in Escherichia coli Rhodobacter capsulatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.18.1.2 0.8
-
 NADPH diaphorase activity, pH 8.0 Rhodobacter capsulatus
1.19.1.1 0.009
-
 ferricytochrome c presence of flavodoxin, pH 8.0, temperature not specified in the publication Rhodobacter capsulatus
1.19.1.1 0.08
-
 NADPH substrate ferricyanide, pH 8.0, temperature not specified in the publication Rhodobacter capsulatus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.18.1.2 soluble
-
 Rhodobacter capsulatus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.18.1.2 30000
-
 about, gel filtration Rhodobacter capsulatus
1.19.1.1 30000
-
 gel filtration Rhodobacter capsulatus
1.19.1.1 30138
-
-
 Rhodobacter capsulatus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.18.1.2 reduced ferredoxin + NADP+ Rhodobacter capsulatus
-
 oxidized ferredoxin + NADPH
-
 ?
1.18.1.2 reduced ferredoxin + NADP+ Rhodobacter capsulatus 37b4
-
 oxidized ferredoxin + NADPH
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.18.1.2 Rhodobacter capsulatus Q9L6V3
-
-
1.18.1.2 Rhodobacter capsulatus 37b4 Q9L6V3
-
-
1.19.1.1 Rhodobacter capsulatus Q9L6V3 enzyme catalyzes reactions of both EC 1.18.1.2 and EC 1.19.1.1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.18.1.2 recombinant enzyme from Escherichia coli to homogeneity, native enzyme about 400fold to homogeneity Rhodobacter capsulatus
1.19.1.1
-
 Rhodobacter capsulatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.18.1.2 methyl viologen + NADPH diaphorase activity Rhodobacter capsulatus reduced methyl viologen + NADP+
-
 ?
1.18.1.2 methyl viologen + NADPH diaphorase activity Rhodobacter capsulatus 37b4 reduced methyl viologen + NADP+
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Rhodobacter capsulatus oxidized ferredoxin + NADPH
-
 ?
1.18.1.2 reduced ferredoxin + NADP+
-
 Rhodobacter capsulatus 37b4 oxidized ferredoxin + NADPH
-
 ?
1.18.1.2 reduced flavodoxin + NADP+
-
 Rhodobacter capsulatus oxidized flavodoxin + NADPH + H+
-
 r
1.18.1.2 reduced flavodoxin + NADP+
-
 Rhodobacter capsulatus 37b4 oxidized flavodoxin + NADPH + H+
-
 r
1.19.1.1 2 ferricyanide + NADPH + H+
-
 Rhodobacter capsulatus 2 ferrocyanide + NADP+ + H+
-
 ?
1.19.1.1 2 ferricyanide + NADPH + H+
-
 Rhodobacter capsulatus 37b4 2 ferrocyanide + NADP+ + H+
-
 ?
1.19.1.1 2 ferricytochrome c + NADPH + H+
-
 Rhodobacter capsulatus 2 ferrocytochrome c + NADP+ + H+
-
 ?
1.19.1.1 additional information enzyme additionally shows diaphorase activity which is induced by treatment with methyl viologen Rhodobacter capsulatus ?
-
 ?
1.19.1.1 additional information enzyme additionally shows diaphorase activity which is induced by treatment with methyl viologen Rhodobacter capsulatus 37b4 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.18.1.2 monomer 1 * 30000, about, recombinant and native enzyme, SDS-PAGE Rhodobacter capsulatus
1.19.1.1 monomer 1 * 30000, SDS-PAGE, 1 * 30138, calculated Rhodobacter capsulatus

Synonyms

EC Number Synonyms Comment Organism
1.18.1.2 ferredoxin (flavodoxin)-(reduced) nicotinamide adenine dinucleotide phosphate reductase
-
 Rhodobacter capsulatus
1.18.1.2 ferredoxin (flavodoxin)-NADP(H) reductase
-
 Rhodobacter capsulatus
1.18.1.2 FPR
-
 Rhodobacter capsulatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.18.1.2 0.25
-
 reduced flavodoxin pH 8.0 Rhodobacter capsulatus
1.18.1.2 7.2
-
 NADPH diaphorase activity, pH 8.0 Rhodobacter capsulatus
1.19.1.1 0.25
-
 ferricytochrome c presence of flavodoxin, pH 8.0, temperature not specified in the publication Rhodobacter capsulatus
1.19.1.1 7.2
-
 NADPH substrate ferricyanide, pH 8.0, temperature not specified in the publication Rhodobacter capsulatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.18.1.2 8
-
 assay at Rhodobacter capsulatus

Cofactor

EC Number Cofactor Comment Organism Structure
1.18.1.2 FAD prosthetic group, tightly bound, can be released by heat denaturation Rhodobacter capsulatus
1.18.1.2 NADP+
-
 Rhodobacter capsulatus
1.18.1.2 NADPH
-
 Rhodobacter capsulatus
1.19.1.1 FAD
-
 Rhodobacter capsulatus