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Literature summary extracted from

  • Courtois, F.; Guerard, C.; Thomas, X.; Ploux, O.
    Escherichia coli cyclopropane fatty acid synthase (2004), Eur. J. Biochem., 271, 4769-4778.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.79 overproduced as a His6-tagged protein Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.79 C139S 15% of wild-type activity Escherichia coli
2.1.1.79 C176S 150% of wild-type activity Escherichia coli
2.1.1.79 C354S 63% of wild-type activity Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.1.1.79 5,5'-dithiobis-(2-nitrobenzoic acid) substrate does not protect against inactivation Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.79 0.073
-
 S-adenosyl-L-methionine mutant enzyme C176S Escherichia coli
2.1.1.79 0.088
-
 S-adenosyl-L-methionine mutant enzyme C139S Escherichia coli
2.1.1.79 0.09
-
 S-adenosyl-L-methionine wild-type enzyme Escherichia coli
2.1.1.79 0.105
-
 S-adenosyl-L-methionine mutant enzyme C354S Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.79 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.79 recombinant His6-tagged protein Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.79 0.05
-
-
 Escherichia coli

Storage Stability

EC Number Storage Stability Organism
2.1.1.79 -20°C, pH 7.4, 20 mM phosphate buffer, 50% v/v glycerol, best storage conditions Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.1.1.79 CFAS
-
 Escherichia coli
2.1.1.79 cyclopropane fatty acid synthase
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.1.1.79 0.3
-
 S-adenosyl-L-methionine mutant enzyme C139S Escherichia coli
2.1.1.79 1.6
-
 S-adenosyl-L-methionine mutant enzyme C354S Escherichia coli
2.1.1.79 2.2
-
 S-adenosyl-L-methionine wild-type enzyme Escherichia coli
2.1.1.79 2.7
-
 S-adenosyl-L-methionine mutant enzyme C176S Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.79 7.5
-
-
 Escherichia coli