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Literature summary extracted from

  • Lyon, E.J.; Shima, S.; Buurman, G.; Chowdhuri, S.; Batschauer, A.; Steinbach, K.; Thauer, R.K.
    UV-A/blue-light inactivation of the ‘metal-free’ hydrogenase (Hmd) from methanogenic archaea. The enzyme contains functional iron after all (2004), Eur. J. Biochem., 271, 195-204.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.12.98.2 expressed in Escherichia coli Methanocaldococcus jannaschii

General Stability

EC Number General Stability Organism
1.12.98.2 sensitive to UV-A/blue light, CO protects from inactivation Methanocaldococcus jannaschii
1.12.98.2 sensitive to UV-A/blue light, CO protects from inactivation Methanothermobacter marburgensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.12.98.2 CN-
-
Methanothermobacter marburgensis
1.12.98.2 CO high concentrations Methanocaldococcus jannaschii
1.12.98.2 CO high concentrations, reversible Methanothermobacter marburgensis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.12.98.2 Iron enzyme contains iron bound to a cofactor of unknown structure, one iron per monomer, iron is released from cofactor upon light inactivation Methanocaldococcus jannaschii
1.12.98.2 Iron enzyme contains iron bound to a mercaptoethanol-extractable cofactor of unknown structure, one iron per monomer, iron is released from cofactor upon light inactivation Methanothermobacter marburgensis
1.12.98.2 additional information no iron-sulfur-cluster or nickel Methanocaldococcus jannaschii
1.12.98.2 additional information no iron-sulfur-cluster or nickel Methanothermobacter marburgensis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.12.98.2 40000
-
2 * 40000, SDS-PAGE Methanothermobacter marburgensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.12.98.2 5,10-methenyltetrahydromethanopterin + H2 Methanocaldococcus jannaschii involved in methanogenesis 5,10-methylenetetrahydromethanopterin + H+
-
r
1.12.98.2 5,10-methenyltetrahydromethanopterin + H2 Methanothermobacter marburgensis involved in methanogenesis 5,10-methylenetetrahydromethanopterin + H+
-
r

Organism

EC Number Organism UniProt Comment Textmining
1.12.98.2 Methanocaldococcus jannaschii
-
-
-
1.12.98.2 Methanothermobacter marburgensis
-
DSMZ 2133
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.12.98.2 enzyme is rapidly inactivated upon exposure to oxygen Methanothermobacter marburgensis

Purification (Commentary)

EC Number Purification (Comment) Organism
1.12.98.2 all purification steps are performed in the dark Methanothermobacter marburgensis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.12.98.2 610
-
purified enzyme, pH 6.0, 40°C, under N2 Methanothermobacter marburgensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.12.98.2 5,10-methenyltetrahydromethanopterin + H2
-
Methanocaldococcus jannaschii 5,10-methylenetetrahydromethanopterin + H+
-
r
1.12.98.2 5,10-methenyltetrahydromethanopterin + H2
-
Methanothermobacter marburgensis 5,10-methylenetetrahydromethanopterin + H+
-
r
1.12.98.2 5,10-methenyltetrahydromethanopterin + H2 involved in methanogenesis Methanocaldococcus jannaschii 5,10-methylenetetrahydromethanopterin + H+
-
r
1.12.98.2 5,10-methenyltetrahydromethanopterin + H2 involved in methanogenesis Methanothermobacter marburgensis 5,10-methylenetetrahydromethanopterin + H+
-
r
1.12.98.2 additional information no reduction of methyl viologen or methyl blue Methanocaldococcus jannaschii ?
-
?
1.12.98.2 additional information no reduction of methyl viologen or methyl blue Methanothermobacter marburgensis ?
-
?

Subunits

EC Number Subunits Comment Organism
1.12.98.2 dimer 2 * 40000, SDS-PAGE Methanothermobacter marburgensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.12.98.2 40 65 2.5-fold higher activity at 65°C than at 40°C Methanothermobacter marburgensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.12.98.2 additional information enzyme contains iron bound to a cofactor of unknown structure Methanocaldococcus jannaschii
1.12.98.2 additional information enzyme contains iron bound to a mercaptoethanol-extractable cofactor of unknown structure Methanothermobacter marburgensis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.12.98.2 0.1
-
CN- pH 6.0, 40°C, under N2, substrate concentration of 0.003 mM Methanothermobacter marburgensis
1.12.98.2 0.2
-
CN- pH 6.0, 40°C, under N2, substrate concentration of 0.01 mM Methanothermobacter marburgensis