Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Naraoka, T.; Uchisawa, H.; Mori, H.; Matsue, H.; Chiba, S.; Kimura, A.
    Purification, characterization and molecular cloning of tyrosinase from the cephalopod mollusk,Illex argentinus (2003), Eur. J. Biochem., 270, 4026-4038.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.14.18.1 Trypsin the tyrosinase activity of ST94 is enhanced to about 4times by proteolysis with trypsin to form a protein, termed ST94t Illex argentinus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.18.1 two distinct messages for the squid tyrosinase, represented as cDNA-1 and cDNA-2 are expressed in the ink sac. The subunit of ST94 is synthesized as a premature protein with 625 amino acid residues and an 18-residue signal sequence regionis eliminated to form the mature subunit comprised of 607 amino acid residues with a deduced molecular mass of 68993 Da Illex argentinus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.18.1 kojic acid IC50: 0.048 mM for ST94 and ST94t; IC50: 0.048 mM for ST94 and ST94t Illex argentinus
1.14.18.1 Phenylthiourea IC50: 0.00017 mM for ST94, 0.00018 mM for ST94t; IC50: 0.00017 mM for ST94, 0.00018 mM for ST94t Illex argentinus
1.14.18.1 tropolone IC50: 0.0021 mM for ST94, 0.0022 mM for ST94t; IC50: 0.0021 mM for ST94, 0.0022 mM for ST94t Illex argentinus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.18.1 0.12
-
 tyramine pH 6.8, 25°C, ST94t Illex argentinus
1.14.18.1 0.24
-
 D-Tyr pH 6.8, 25°C, ST94t Illex argentinus
1.14.18.1 0.24
-
 3,4-Dihydroxyphenylpropionic acid pH 6.8, 25°C, ST94t Illex argentinus
1.14.18.1 0.32
-
 tyramine pH 6.8, 25°C, ST94 Illex argentinus
1.14.18.1 0.32
-
 D-Tyr pH 6.8, 25°C, ST94 Illex argentinus
1.14.18.1 0.35
-
 L-Tyr pH 6.8, 25°C, ST94t Illex argentinus
1.14.18.1 0.39
-
 dopamine pH 6.8, 25°C, ST94t Illex argentinus
1.14.18.1 0.49
-
 3,4-Dihydroxyphenylpropionic acid pH 6.8, 25°C, ST94 Illex argentinus
1.14.18.1 0.57
-
 L-Tyr pH 6.8, 25°C, ST94 Illex argentinus
1.14.18.1 1.3
-
 dopamine pH 6.8, 25°C, ST94 Illex argentinus
1.14.18.1 2.7
-
 D-Dopa pH 6.8, 25°C, ST94t Illex argentinus
1.14.18.1 3.2
-
 D-Dopa pH 6.8, 25°C, ST94 Illex argentinus
1.14.18.1 6.5
-
 L-Dopa pH 6.8, 25°C, ST94t Illex argentinus
1.14.18.1 6.8
-
 L-Dopa pH 6.8, 25°C, ST94 Illex argentinus
1.14.18.1 7.3
-
 pyrocatechol pH 6.8, 25°C, ST94t Illex argentinus
1.14.18.1 9.3
-
 pyrocatechol pH 6.8, 25°C, ST94 Illex argentinus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.18.1 copper contains 2 copper atoms per subunit Illex argentinus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.18.1 63800
-
 2 * 63800, ST94t Illex argentinus
1.14.18.1 70000
-
 2 * 70000, ST94, SDS-PAGE Illex argentinus
1.14.18.1 127600
-
 St94t Illex argentinus
1.14.18.1 140200
-
 ST94, MALDI-TOF mass spectrometry Illex argentinus

Organism

EC Number Organism UniProt Comment Textmining
1.14.18.1 Illex argentinus Q76DT9 tyrosinase precursor 2
-
1.14.18.1 Illex argentinus Q76DU0 tyrosinase precursor 1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.18.1
-
 Illex argentinus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.14.18.1 ink
-
 Illex argentinus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.18.1 3,4-dihydroxyphenylpropionic acid + O2
-
 Illex argentinus 3-(3,4-dioxocyclohexa-1,5-dien-1-yl)propionic acid
-
 ?
1.14.18.1 D-dopa + 1/2 O2
-
 Illex argentinus D-dopaquinone + H2O
-
 ?
1.14.18.1 D-Tyr + O2
-
 Illex argentinus D-dopa + H2O
-
 ?
1.14.18.1 dopamine + 1/2 O2
-
 Illex argentinus dopamine quinone + H2O
-
 ?
1.14.18.1 L-dopa + 1/2 O2
-
 Illex argentinus L-dopaquinone + H2O
-
 ?
1.14.18.1 L-Tyr + O2 + AH2
-
 Illex argentinus L-dopa + H2O + A
-
 ?
1.14.18.1 pyrocatechol + O2
-
 Illex argentinus ?
-
 ?
1.14.18.1 tyramine + O2
-
 Illex argentinus 4-(2-aminoethyl)cyclohexa-3,5-diene-1,2-dione + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.18.1 dimer 2 * 63800, ST94t Illex argentinus
1.14.18.1 dimer 2 * 70000, ST94, SDS-PAGE Illex argentinus

Synonyms

EC Number Synonyms Comment Organism
1.14.18.1 ST94
-
 Illex argentinus
1.14.18.1 ST94t the tyrosinase activity of ST94 is enhanced by proteolysis with trypsin to form a protein, termed ST94t Illex argentinus
1.14.18.1 tyrosinase
-
 Illex argentinus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.14.18.1 30
-
 pH 7.4, 20 min, stable up to Illex argentinus
1.14.18.1 50
-
 pH 7.4, 20 min, about 20% loss of activity Illex argentinus
1.14.18.1 55
-
 pH 7.4, 20 min, about 40% loss of activity Illex argentinus
1.14.18.1 60
-
 pH 7.4, 20 min, about 80% loss of activity Illex argentinus
1.14.18.1 70
-
 pH 7.4, 20 min, complete inactivation Illex argentinus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.18.1 8
-
-
 Illex argentinus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.18.1 6 9 pH 6.0: about 55% of maximal activity, pH 9.0: about 45% of maximal activity Illex argentinus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.14.18.1 6.5 11 4°C, 24 h, less than 10% loss of activity Illex argentinus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.18.1 Illex argentinus isoelectric focusing
-
 4.1

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.14.18.1 0.00017
-
 IC50: 0.00017 mM for ST94, 0.00018 mM for ST94t Illex argentinus Phenylthiourea
1.14.18.1 0.0021
-
 IC50: 0.0021 mM for ST94, 0.0022 mM for ST94t Illex argentinus tropolone
1.14.18.1 0.048
-
 IC50: 0.048 mM for ST94 and ST94t Illex argentinus kojic acid