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Literature summary extracted from

  • Wattebled, F.; Buleon, A.; Bouchet, B.; Ral, J.P.; Lienard, L.; Delvalle, D.; Binderup, K.; Dauvillee, D.; Ball, S.; D'Hulst, C.
    Granule-bound starch synthase I. A major enzyme involved in the biogenesis of B-crystallites in starch granules (2002), Eur. J. Biochem., 269, 3810-3820.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.242 GBSSI, DNA sequence determination and analysis, expression in Escherichia coli, complementation of the sta2-1 mutation, phylogenetic tree Chlamydomonas reinhardtii

Protein Variants

EC Number Protein Variants Comment Organism
2.4.1.242 additional information the sta2-29::ARG7 mutation causes loss of GBSSI activity, the Sta2-1 mutation results in production of a truncated 58 kDa GBSSI isozyme Chlamydomonas reinhardtii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.242 58000
-
 x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE Chlamydomonas reinhardtii
2.4.1.242 69000
-
 x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE Chlamydomonas reinhardtii
2.4.1.242 76000
-
 x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE Chlamydomonas reinhardtii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n Chlamydomonas reinhardtii i.e. starch, composition of starch granule, including amylopectin, amylose, A-type and B-type crystals ADP + (1,4-alpha-glucosyl)n+1
-
 ?
2.4.1.242 additional information Chlamydomonas reinhardtii decreased amylose synthesis activity and altered starch granule morphology during nitrogen starvation, overview ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.242 Chlamydomonas reinhardtii O64925 complete cDNA
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.242 proteolytic modification the transit peptide of the immature isozyme GBSSI is cleaved off resulting in the mature enzyme Chlamydomonas reinhardtii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n i.e. starch, composition of starch granule, including amylopectin, amylose, A-type and B-type crystals Chlamydomonas reinhardtii ADP + (1,4-alpha-glucosyl)n+1
-
 ?
2.4.1.242 ADP-glucose + (1,4-alpha-glucosyl)n i.e. starch Chlamydomonas reinhardtii ADP + (1,4-alpha-glucosyl)n+1
-
 ?
2.4.1.242 additional information decreased amylose synthesis activity and altered starch granule morphology during nitrogen starvation, overview Chlamydomonas reinhardtii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.242 ? x * 76000, immature wild-type GBSSI, SDS-PAGE, x * 69000, mature wild-type GBSSI, SDS-PAGE, x * 58000, truncated mutant sta2-1, SDS-PAGE Chlamydomonas reinhardtii

Synonyms

EC Number Synonyms Comment Organism
2.4.1.242 GBSSI
-
 Chlamydomonas reinhardtii
2.4.1.242 granule-bound starch synthase I
-
 Chlamydomonas reinhardtii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.242 30
-
 assay at Chlamydomonas reinhardtii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.242 9
-
 assay at Chlamydomonas reinhardtii