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Literature summary extracted from

  • Brenk, R.; Stubbs, M.T.; Heine, A.; Reuter, K.; Klebe, G.
    Flexible adaptations in the structure of the tRNA-modifying enzyme tRNA-guanine transglycosylase and their implications for substrate selectivity, reaction mechanism and structure-based drug design (2003), ChemBioChem, 4, 1066-1077.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.64 wild-type and mutant enzyme Y106F, hanging drop method, crystals with a size of approximately 0.7 * 0.7 * 0.2 mm grew within five to seven days Zymomonas mobilis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.64 Y106F 2.8fold decrease in turnover-number for guanine and tRNA, 2.8fold decrease in Km-value for tRNA, 3.4fold increase in turnover-number for guanine, in contrast to Phe106 the residue Tyr106 is hydrogen bonded in the ligand-free structure, this is not the reason for the kinetic difference, other structurally less evident factors are responsible for this behaviour Zymomonas mobilis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.64 2,6-diamino-8-[[(1H-imidazol-2-yl)sulfanyl]methyl]quinazolin-4(3H)-one
-
 Zymomonas mobilis
2.4.2.64 2-butyl-5,6-dihydro-1H-imidazo[4,5-d]pyridazine-4,7-dione
-
 Zymomonas mobilis
2.4.2.64 4-aminophthalhydrazide
-
 Zymomonas mobilis
2.4.2.64 5,7-diamino-2,3-dihydro-phthalazine-1,4-dione
-
 Zymomonas mobilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.64 0.00036
-
 guanine34 in tRNA mutant enzyme Y106F Zymomonas mobilis
2.4.2.64 0.00038
-
 guanine wild-type enzyme Zymomonas mobilis
2.4.2.64 0.001
-
 guanine34 in tRNA wild-type enzyme Zymomonas mobilis
2.4.2.64 0.0013
-
 guanine mutant enzyme Y106F Zymomonas mobilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.64 guanine34 in tRNA + queuine Zymomonas mobilis catalyses a base-exchange reaction that leads to anticodon modifications of certain tRNAs queuine34 in tRNA + guanine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.64 Zymomonas mobilis P28720 wild-type and Y106F mutant
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.64 guanine34 in tRNA + queuine
-
 Zymomonas mobilis queuine34 in tRNA + guanine
-
 ?
2.4.2.64 guanine34 in tRNA + queuine catalyses a base-exchange reaction that leads to anticodon modifications of certain tRNAs Zymomonas mobilis queuine34 in tRNA + guanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.2.64 TGT
-
 Zymomonas mobilis
2.4.2.64 tRNA-guanine transglycosylase
-
 Zymomonas mobilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.64 0.004
-
 guanine mutant enzyme Y106F Zymomonas mobilis
2.4.2.64 0.005
-
 guanine34 in tRNA mutant enzyme Y106F Zymomonas mobilis
2.4.2.64 0.011
-
 guanine wild-type enzyme Zymomonas mobilis
2.4.2.64 0.014
-
 guanine34 in tRNA wild-type enzyme Zymomonas mobilis