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Literature summary extracted from

  • Hwang, B.Y.; Lee, H.J.; Yang, Y.H.; Joo, H.S.; Kim, B.G.
    Characterization and investigation of substrate specificity of the sugar aminotransferase WecE from E. coli K12 (2004), Chem. Biol., 11, 915-925.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.33 gene WecC, amino acid sequence determination and analysis, overexpression of C-terminally His6-tagged enzyme in strain BL21(DE3), phylogenetic analysis Escherichia coli
2.6.1.59 expression in Escherichia coli BL21 Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.33 (aminooxy)acetic acid complete inhibition by a mixture of hydroxylamine, (aminooxy)acetic acid, and gabaculine as inhibitors specific for pyridoxal 5'-phosphate-dependent enzymes Escherichia coli
2.6.1.33 gabaculine complete inhibition by a mixture of hydroxylamine, (aminooxy)acetic acid, and gabaculine as inhibitors specific for pyridoxal 5'-phosphate-dependent enzymes Escherichia coli
2.6.1.33 hydroxylamine complete inhibition by a mixture of hydroxylamine, (aminooxy)acetic acid, and gabaculine as inhibitors specific for pyridoxal 5'-phosphate-dependent enzymes Escherichia coli
2.6.1.59 (aminooxy)acetic acid
-
 Escherichia coli
2.6.1.59 gabaculine
-
 Escherichia coli
2.6.1.59 hydroxylamine
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.33 additional information
-
 additional information kinetics in a coupled assay Escherichia coli
2.6.1.59 0.11
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.5, 25°C Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.33 44000
-
 4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry Escherichia coli
2.6.1.33 44452
-
 4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry Escherichia coli
2.6.1.33 44465
-
 4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry Escherichia coli
2.6.1.33 180000
-
 recombinant His6-tagged enzyme, gel filtration Escherichia coli
2.6.1.59 44000
-
 4 * 44000, SDS-PAGE Escherichia coli
2.6.1.59 180000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.33 Escherichia coli
-
 gene WecC
-
2.6.1.59 Escherichia coli P27833
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.33 recombinant C-terminally His6-tagged enzyme from strain BL21(DE3) by nickel chelate and hydrophobic interaction chromatography Escherichia coli
2.6.1.59
-
 Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.33 dTDP-4-amino-4,6-dideoxy-alpha-D-glucose + 2-oxoglutarate = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + L-glutamate substrate specificity might be related to conserved motifs VLalpha, VLbeta, and VLgamma, around the active site Escherichia coli
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.33 additional information
-
 activity measurement in a coupled assay with NADH-dependent L-GDH Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
 Escherichia coli dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
2.6.1.33 dTDP-4-dehydro-6-deoxy-D-mannose + L-glutamate
-
 Escherichia coli dTDP-4-amino-6-deoxy-D-mannose + 2-oxoglutarate
-
 ?
2.6.1.33 additional information no activity with dGDP-4-keto-6-deoxy-D-mannose, the nucleotide moiety is a key determinant for acceptor substrate specificity Escherichia coli ?
-
 ?
2.6.1.59 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamate
-
 Escherichia coli dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutarate
-
 ?
2.6.1.59 dTDP-4-dehydro-6-deoxy-D-glucose + L-glutamine 65% of the activity with L-glutamate Escherichia coli dTDP-4-amino-4,6-dideoxy-D-galactose + 2-oxoglutaramate
-
 ?
2.6.1.59 dTDP-4-dehydro-6-deoxy-D-mannose + L-glutamate
-
 Escherichia coli ? + 2-oxoglutarate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.33 tetramer 4 * 44000, recombinant His6-tagged enzyme, SDS-PAGE, 4 * 44452, recombinant His6-tagged enzyme, nucleotide sequence calculation, 4 * 44465, recombinant His6-tagged enzyme, MALDI-TOF mass spectrometry Escherichia coli
2.6.1.59 tetramer 4 * 44000, SDS-PAGE Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
2.6.1.33 SAT
-
 Escherichia coli
2.6.1.33 sugar aminotransferase
-
 Escherichia coli
2.6.1.33 WecE
-
 Escherichia coli
2.6.1.59 WecE
-
 Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.33 37
-
-
 Escherichia coli
2.6.1.59 37
-
-
 Escherichia coli

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.6.1.33 20 45 25% of maximal activity at 45°C Escherichia coli
2.6.1.59 20 45
-
 Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.59 0.38
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.5, 25°C Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.33 7.5
-
-
 Escherichia coli
2.6.1.59 7.5
-
-
 Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.6.1.33 5 9.5 enzyme shows over 80% of maximal activity at pH 6.0 and pH 8.0, and very low activity below pH 5.0 and above pH 9.5 Escherichia coli
2.6.1.59 6 8
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.33 pyridoxal 5'-phosphate dependent on Escherichia coli
2.6.1.59 pyridoxal 5'-phosphate
-
 Escherichia coli

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
2.6.1.59 3.62
-
 dTDP-4-dehydro-6-deoxy-D-glucose pH 7.5, 25°C Escherichia coli