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Literature summary extracted from

  • Shen, X.H.; Liu, Z.P.; Liu, S.J.
    Functional identification of the gene locus (ncg1231) and characterization of catechol 1,2-dioxygenase in Corynebacterium glutamicum (2004), Biotechnol. Lett., 26, 575-580.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.1 expression in Escherichia coli JM109 Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.1 0.00424
-
 catechol
-
 Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.13.11.1 39000
-
 2 * 39000, SDS-PAGE Corynebacterium glutamicum
1.13.11.1 63500
-
 gel filtration Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.1 Corynebacterium glutamicum
-
 ATCC 13032
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.13.11.1
-
 Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.13.11.1 0.14
-
 cells cultivated with benzoate Corynebacterium glutamicum
1.13.11.1 0.16
-
 cells cultivated with phenol Corynebacterium glutamicum
1.13.11.1 5.4
-
 purified recombinant enzyme Corynebacterium glutamicum

Storage Stability

EC Number Storage Stability Organism
1.13.11.1 4°C, pH 7.6, 50 mM Tris/HCl, stable for two weeks Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.1 3-methylcatechol + O2
-
 Corynebacterium glutamicum 2-methyl-cis,cis-muconate
-
 ?
1.13.11.1 4-methylcatechol + O2
-
 Corynebacterium glutamicum 3-methyl-cis,cis-muconate
-
 ?
1.13.11.1 catechol + O2
-
 Corynebacterium glutamicum cis,cis-muconate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.13.11.1 dimer 2 * 39000, SDS-PAGE Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.1 30
-
-
 Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.1 9
-
-
 Corynebacterium glutamicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.13.11.1 Corynebacterium glutamicum isoelectric focusing
-
 5.2