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Literature summary extracted from

  • Kaya, M.; Matsumura, K.; Higashida, K.; Hata, Y.; Kawato, A.; Abe, Y.; Akita, O.; Takaya, N.; Shoun, H.
    Cloning and enhanced expression of the cytochrome P450nor gene (nicA; CYP55A5) encoding nitric oxide reductase from Aspergillus oryzae (2004), Biosci. Biotechnol. Biochem., 68, 2040-2049.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.5 overexpression in Aspergillus oryzae Aspergillus oryzae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.1.14 0.801
-
NADH in 50 mM sodium phosphate (pH 7.2), at 37°C Aspergillus oryzae
1.7.1.14 0.934
-
NADPH in 50 mM sodium phosphate (pH 7.2), at 37°C Aspergillus oryzae
1.7.2.5 0.2
-
NO 37°C, pH 7.2 Aspergillus oryzae
1.7.2.5 0.8
-
NADH 37°C, pH 7.2 Aspergillus oryzae
1.7.2.5 0.93
-
NADPH 37°C, pH 7.2 Aspergillus oryzae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.7.1.14 45000
-
SDS-PAGE Aspergillus oryzae
1.7.1.14 45690
-
deduced from amino acid sequence Aspergillus oryzae
1.7.2.5 45000
-
x * 45000, SDS-PAGE Aspergillus oryzae
1.7.2.5 45689
-
x * 45689, deduced from nucleotide sequence Aspergillus oryzae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.7.2.5 NO + NADH Aspergillus oryzae
-
N2O + H2O + NAD+
-
?
1.7.2.5 NO + NADH Aspergillus oryzae OSI 1013
-
N2O + H2O + NAD+
-
?
1.7.2.5 NO + NADPH Aspergillus oryzae
-
N2O + H2O + NADP+
-
?
1.7.2.5 NO + NADPH Aspergillus oryzae OSI 1013
-
N2O + H2O + NADP+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.14 Aspergillus oryzae Q8NKB4
-
-
1.7.1.14 Aspergillus oryzae OSI 1013 Q8NKB4
-
-
1.7.2.5 Aspergillus oryzae Q8NKB4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.1.14 ammonium sulfate precipitation, Resource Q column chromatography, Resource PHE column chromatography, and Superdex 200 gel filtration Aspergillus oryzae
1.7.2.5 ammonium sulfate, Resource Q, Resource PHE, Superdex 200 Aspergillus oryzae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.2.5 75
-
-
Aspergillus oryzae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.14 NO + NADH + H+
-
Aspergillus oryzae N2O + NAD+ + H2O
-
?
1.7.1.14 NO + NADH + H+
-
Aspergillus oryzae OSI 1013 N2O + NAD+ + H2O
-
?
1.7.1.14 NO + NADPH + H+
-
Aspergillus oryzae N2O + NADP+ + H2O
-
?
1.7.1.14 NO + NADPH + H+
-
Aspergillus oryzae OSI 1013 N2O + NADP+ + H2O
-
?
1.7.2.5 NO + NADH
-
Aspergillus oryzae N2O + H2O + NAD+
-
?
1.7.2.5 NO + NADH NADH is preferred over NADPH Aspergillus oryzae N2O + H2O + NAD+
-
?
1.7.2.5 NO + NADH
-
Aspergillus oryzae OSI 1013 N2O + H2O + NAD+
-
?
1.7.2.5 NO + NADH NADH is preferred over NADPH Aspergillus oryzae OSI 1013 N2O + H2O + NAD+
-
?
1.7.2.5 NO + NADPH
-
Aspergillus oryzae N2O + H2O + NADP+
-
?
1.7.2.5 NO + NADPH
-
Aspergillus oryzae OSI 1013 N2O + H2O + NADP+
-
?

Subunits

EC Number Subunits Comment Organism
1.7.2.5 ? x * 45000, SDS-PAGE Aspergillus oryzae
1.7.2.5 ? x * 45689, deduced from nucleotide sequence Aspergillus oryzae

Synonyms

EC Number Synonyms Comment Organism
1.7.1.14 Anor
-
Aspergillus oryzae
1.7.1.14 cytochrome P450nor
-
Aspergillus oryzae
1.7.2.5 Anor
-
Aspergillus oryzae

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.2.5 166.7
-
NO 37°C, PH 7.2 Aspergillus oryzae

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.14 heme
-
Aspergillus oryzae
1.7.1.14 NADH the enzyme employs both NADH and NADPH as electron donors Aspergillus oryzae
1.7.1.14 NADPH the enzyme employs both NADH and NADPH as electron donors Aspergillus oryzae
1.7.2.5 NADH
-
Aspergillus oryzae
1.7.2.5 NADPH
-
Aspergillus oryzae